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Characterization of the Lung Seven Transmembrane (LUSTR) Protein Family

Title: Characterization of the Lung Seven Transmembrane (LUSTR) Protein Family.
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Name(s): Middlebrooks, Jennifer Ivey, author
Keller, Laura R., professor directing dissertation
Levenson, Cathy, university representative
Keller, Tom, committee member
McGinnis, Karen, committee member
Deng, Wumin, committee member
Department of Biological Science, degree granting department
Florida State University, degree granting institution
Type of Resource: text
Genre: Text
Issuance: monographic
Date Issued: 2012
Publisher: Florida State University
Florida State University
Place of Publication: Tallahassee, Florida
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Cilia are recognized as an important sensory structure for the cell. However, much about the signaling involved in ciliary outgrowth remains a mystery. Chlamydomonas, a green algae, is a model for ciliary and flagellar studies owing to its pair of anterior flagella and haploid genome. In a previous study of gene expression during flagellar outgrowth in Chlamydomonas after environmental shock, the gene encoding a predicted seven transmembrane protein (Cr7TM) was differentially expressed. Subsequent experiments described here have attempted to elucidate the function of Cr7TM in the cell and specifically in the flagella. Bioinformatic search techniques and predictions of the evolutionary history of Cr7TM and its homologues imply that this LUSTR protein family is unique in both its primary structure and mechanisms for protein interaction. It is also highly conserved and likely involved in a critical cellular function. LUSTR proteins are ubiquitously expressed across metazoan organisms and tissue types. Also, Cr7TM is differentially expressed during outgrowth of cilia/flagella but not during oxidative stress, indicating Cr7TM's involvement in flagellar outgrowth rather than the stress response. Fluorescence microscopy indicates that Cr7TM co-localizes with acetylated á-tubulin in the flagella. Creation of an inducible knockdown mutant line of Cr7TM allowed for comparison of the phenotypes between induced and uninduced RNAi knockdown mutant lines. The Cr7TM knockdown line demonstrates a diminished cell size along with a rapid increase in the density of cell cultures as compared to the control, indicating a possible cellular role for Cr7TM in cell cycling or mitotic regulation. Additionally, imaging of human embryonic kidney cells shows colocalization of LUSTR proteins near the highly conserved centriole and basal body organelles. When taken together the high degree of sequence conservation, knockdown phenotype and cellular localization of LUSTR proteins provide evidence for LUSTR involvement in the regulation of cell cycle.
Identifier: FSU_migr_etd-5800 (IID)
Submitted Note: A Dissertation submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
Degree Awarded: Summer Semester, 2012.
Date of Defense: June 1, 2012.
Keywords: CHLAMYDOMONAS, CILIA, FLAGELLA, GPR107, LUSTR, TRANSMEMBRANE
Bibliography Note: Includes bibliographical references.
Advisory Committee: Laura R. Keller, Professor Directing Dissertation; Cathy Levenson, University Representative; Tom Keller, Committee Member; Karen McGinnis, Committee Member; Wumin Deng, Committee Member.
Subject(s): Biology
Life sciences
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_etd-5800
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Host Institution: FSU

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Middlebrooks, J. I. (2012). Characterization of the Lung Seven Transmembrane (LUSTR) Protein Family. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-5800