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Crosstalk between Chromatin Structure and DNA Methylation and the Regulation of DNA Templated Processes

Title: Crosstalk between Chromatin Structure and DNA Methylation and the Regulation of DNA Templated Processes.
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Name(s): Noori, Tarreq, author
Biological Science
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2011
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Methylation of DNA is one of the earliest described epigenetic modifications. Hypermethylation is associated with gene silencing, while the inhibition of methylation is generally associated with reactivating silenced genes. The packaging of DNA in the nucleus into chromatin also plays a role in regulating gene expression. We sought to understand the crosstalk between changes in methylation status of the genome and changes in chromatin structure. 5-azacytidine (5-azaC), a potent DNA methytransferase inhibitor, has recently generated interest as a potential anti-cancer drug, possibly functioning by reactivating silenced tumor suppressor genes. We treated the hematologic cancer cell lines U-937 and T-HP1 with 5-aza for varying lengths of time. We then harvested DNA for methylation studies, RNA for gene expression studies and chromatin for nuclease accessibility studies. The chromatin accessibility was further measured at two different levels of resolution; the domain level (10s of kb) and nucleosome position (10s of bp). This was achieved using an innovative DNA microarray assay. Results were analyzed to correlate changes in chromatin structure with changes in methylation and gene expression. We have identified that the class II, major histocompatibility complex, transactivator (CIITA) shows chromatin structural changes. The results provide a model for understanding the regulatory chromatin structure involved in the immune response.
Identifier: FSU_migr_uhm-0032 (IID)
Keywords: Nucleosome distribution, DNA methyltransferase inhibitor, Microarray
Submitted Note: A Thesis submitted to the Department of Biological Science in partial fulfillment of the requirements for graduation with Honors in the Major.
Degree Awarded: Spring Semester, 2011.
Date of Defense: April 13, 2011.
Subject(s): Biology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0032
Restrictions on Access: http://creativecommons.org/licenses/by-nc-nd/3.0/
Host Institution: FSU
Is Part of Series: Honors Theses.

Choose the citation style.
Noori, T. (2011). Crosstalk between Chromatin Structure and DNA Methylation and the Regulation of DNA Templated Processes. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0032