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Mutagenesis of the Crystal Contact of Acidic Fibroblast Growth Factor

Title: Mutagenesis of the Crystal Contact of Acidic Fibroblast Growth Factor.
Name(s): Honjo, Eijiro, author
Tamada, Taro, author
Adachi, Motoyasu, author
Kuroki, Ryota, author
Meher, Akshaya, author
Blaber, Michael, author
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2008
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: An attempt has been made to improve a crystal contact of human acidic fibroblast growth factor (haFGF; 140 amino acids) to control the crystal growth, because haFGF crystallizes only as a thin-plate form, yielding crystals suitable for X-ray but not neutron diffraction. X-ray crystal analysis of haFGF showed that the Glu81 side chain, located at a crystal contact between haFGF molecules, is in close proximity with an identical residue related by crystallographic symmetry, suggesting that charge repulsion may disrupt suitable crystal-packing interactions. To investigate whether the Glu residue affects the crystal-packing interactions, haFGF mutants in which Glu81 was replaced by Ala, Val, Leu, Ser and Thr were constructed. Although crystals of the Ala and Leu mutants were grown as a thin-plate form by the same precipitant (formate) as the wild type, crystals of the Ser and Thr mutants were grown with increased thickness, yielding a larger overall crystal volume. X-ray structural analysis of the Ser mutant determined at 1.35 A resolution revealed that the hydroxy groups of Ser are linked by hydrogen bonds mediated by the formate used as a precipitant. This approach to engineering crystal contacts may contribute to the development of large protein crystals for neutron crystallography.
Identifier: FSU_migr_biomed_faculty_publications-0007 (IID)
Keywords: acidic fibroblast growth factor, crystal contacts, mutagenesis
Uncontrolled subjects: Crystallography, X-Ray, Fibroblast Growth Factor 1, Models, Molecular, Mutagenesis, Protein Conformation
Note: Originally published in Journal of Synchrotron Radiation
Citation: Honjo, E., Tamada, T., Adachi, M., Kuroki, R., Meher, A. and Blaber, M., J. (2008). Mutagenesis of the crystal contact of acidic fibroblast growth factor. Synchrotron Radiation 15, 285-287.
Subject(s): Biochemistry
Molecular biology
Developmental biology
Clinical biochemistry
Medical sciences
Persistent Link to This Record:
Owner Institution: FSU
Is Part of Series: Department of Biomedical Sciences Faculty Publications.
Is Part Of: Journal of Synchrotron Radiation.
Issue: Pt 3, 15

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Honjo, E., Tamada, T., Adachi, M., Kuroki, R., Meher, A., & Blaber, M. (2008). Mutagenesis of the Crystal Contact of Acidic Fibroblast Growth Factor. Journal Of Synchrotron Radiation. Retrieved from