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Completed KLK Activome Profile

Title: A Completed KLK Activome Profile: Investigation of Activation Profiles of KLK9, 10, and 15.
Name(s): Yoon, Hyesook, 1975-, author
Blaber, Sachiko, author
Debela, Mekdes, author
Goettig, Peter, author
Scarisbrick, Isobel, author
Blaber, Michael, author
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2009
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: We previously reported the activation profiles of the human kallikrein-related peptidases (KLKs) as determined from a KLK pro-peptide fusion-protein system. That report described the activity profiles of 12 of the 15 mature KLKs versus the 15 different pro-KLK sequences. The missing profiles in the prior report, involving KLK9, 10, and 15, are now described. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, mass spectrometry, and N-terminal sequence analyses show that KLK9 and 10 exhibit low hydrolytic activities towards all of the 15 pro-KLK sequences, while KLK15 exhibits significant activity towards both Arg- and Lys-containing KLK pro-sequences. The ability of KLK15 to activate pro-KLK8, 12, and 14 is confirmed using recombinant pro-KLK proteins, and shown to be significant for activation of pro-KLK8 and 14, but not 12. These additional data for KLK9, 10, and 15 now permit a completed KLK activome profile, using a KLK pro-peptide fusion-protein system, to be described. The results suggest that KLK15, once activated, can potentially feed back into additional pro-KLK activation pathways. Conversely, KLK9 and 10, once activated, are unlikely to participate in further pro-KLK activation pathways, although similar to KLK1 they may activate other bioactive peptides.
Identifier: FSU_migr_biomed_faculty_publications-0012 (IID)
Keywords: activation cascade, activome, kallikrein, kallikrein-related peptidases (KLKs), protease
Uncontrolled subjects: Electrophoresis, Agar Gel, Gene Expression Profiling, Humans, Kallikreins, Recombinant Fusion Proteins
Note: Originally published in Biological Chemistry.
Citation: Yoon, H., Blaber, S.I., Debela, M., Goettig, P., Scarisbrick, I.A. and Blaber, M. (2009). A completed KLK activome profile: Investigation of activation profiles of KLK9, 10 and 15. Biological Chemistry 390, 373-377.
Subject(s): Biochemistry
Molecular biology
Developmental biology
Clinical biochemistry
Medical sciences
Persistent Link to This Record:
Owner Institution: FSU
Is Part of Series: Department of Biomedical Sciences Faculty Publications.
Is Part Of: Biological Chemistry.
Issue: 4, 390

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Yoon, H., Blaber, S., Debela, M., Goettig, P., Scarisbrick, I., & Blaber, M. (2009). A Completed KLK Activome Profile: Investigation of Activation Profiles of KLK9, 10, and 15. Biological Chemistry. Retrieved from