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Collagen XIV Is Important for Growth and Structural Integrity of the Myocardium

Title: Collagen XIV Is Important for Growth and Structural Integrity of the Myocardium.
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Name(s): Tao, Ge, author
Levay, Agata, author
Peacock, Jacqueline, author
Huk, Danielle, author
Both, Sarah, author
Purcell, Nicole, author
Pinto, Jose, author
Galantowicz, Maarten, author
Koch, Manuel, author
Lucchesi, Pamela, author
Birk, David E., author
Lincoln, Joy, author
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2012
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Collagen XIV is a fibril-associated collagen with an interrupted triple helix (FACIT). Previous studies have shown that this collagen type regulates early stages of fibrillogenesis in connective tissues of high mechanical demand. Mice null for Collagen XIV are viable, however formation of the interstitial collagen network is defective in tendons and skin leading to reduced biomechanical function. The assembly of a tightly regulated collagen network is also required in the heart, not only for structural support but also for controlling cellular processes. Collagen XIV is highly expressed in the embryonic heart, notably within the cardiac interstitium of the developing myocardium, however its role has not been elucidated. To test this, we examined cardiac phenotypes in embryonic and adult mice devoid of Collagen XIV. From as early as E11.5, Col14a1(-/-) mice exhibit significant perturbations in mRNA levels of many other collagen types and remodeling enzymes (MMPs, TIMPs) within the ventricular myocardium. By post natal stages, collagen fibril organization is in disarray and the adult heart displays defects in ventricular morphogenesis. In addition to the extracellular matrix, Col14a1(-/-) mice exhibit increased cardiomyocyte proliferation at post natal, but not E11.5 stages, leading to increased cell number, yet cell size is decreased by 3 months of age. In contrast to myocytes, the number of cardiac fibroblasts is reduced after birth associated with increased apoptosis. As a result of these molecular and cellular changes during embryonic development and post natal maturation, cardiac function is diminished in Col14a1(-/-) mice from 3 months of age; associated with dilation in the absence of hypertrophy, and reduced ejection fraction. Further, Col14a1 deficiency leads to a greater increase in left ventricular wall thickening in response to pathological pressure overload compared to wild type animals. Collectively, these studies identify a new role for type XIV collagen in the formation of the cardiac interstitium during embryonic development, and highlight the importance of the collagen network for myocardial cell survival, and function of the working myocardium after birth.
Identifier: FSU_migr_biomed_faculty_publications-0056 (IID), 10.1016/j.yjmcc.2012.08.002 (DOI)
Keywords: Collagen XIV, myocardium, extracellular matrix, cardiomyocytes, cardiac fibroblasts
Uncontrolled subjects: Animals, Cell Proliferation, Collagen, Glycoproteins, Heart, Heart Ventricles, Hypertrophy, Left Ventricular, Male, Mice, Mice, Transgenic, Myocardial Contraction, Myocardium, Myocytes, Cardiac, Stroke Volume, Transcription, Genetic, Ventricular Function, Left, Ventricular Pressure, Ventricular Remodeling
Note: Originally published in Journal'>http://www.sciencedirect.com/science/article/pii/S002228281200291X">Journal of Molecular and Cellular Cardiology.
Citation: Tao G, Levay AK, Peacock JD, Huk DJ, Both SN, Purcell NH, Pinto JR, Galantowicz ML, Koch M, Lucchesi PA, Birk DE, & Lincoln J. (2012). Collagen XIV is important for growth and structural integrity of the myocardium. J Mol Cell Cardiol. 2012 Nov;53(5):626-38. doi: 10.1016/j.yjmcc.2012.08.002.
Subject(s): Amino acids
Peptides
Proteins
Carbohydrates
Cardiovascular system -- Diseases
Cardiovascular system
Cells
Cytology
Molecules
Diagnosis
Macromolecules
Medical sciences
Musculoskeletal system
Pathology
Symptoms
Links: http://dx.doi.org/10.1016/j.yjmcc.2012.08.002
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_biomed_faculty_publications-0056
Owner Institution: FSU
Is Part of Series: Department of Biomedical Sciences Faculty Publications.
Is Part Of: Journal of Molecular and Cellular Cardiology.
Issue: 5, 53

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Tao, G., Levay, A., Peacock, J., Huk, D., Both, S., Purcell, N., … Lincoln, J. (2012). Collagen XIV Is Important for Growth and Structural Integrity of the Myocardium. Journal Of Molecular And Cellular Cardiology. Retrieved from http://dx.doi.org/10.1016/j.yjmcc.2012.08.002