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Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces

Title: Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces.
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Name(s): Newcomer, Robert, author
Moussallem, Maroun, author
Keller, Thomas C. S., author
Schlenoff, Joseph B., author
Sang, Qing-Xiang, author
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2011
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Under normal physiological conditions, mature human coronary artery smooth muscle cells (hCASMCs) exhibit a "contractile" phenotype marked by low rates of proliferation and protein synthesis, but these cells possess the remarkable ability to dedifferentiate into a "synthetic" phenotype when stimulated by conditions of pathologic stress. A variety of polyelectrolyte multilayer (PEMU) films are shown here to exhibit bioactive properties that induce distinct responses from cultured hCASMCs. Surfaces terminated with Nafion or poly(styrenesulfonic acid) (PSS) induce changes in the expression and organization of intracellular proteins, while a hydrophilic, zwitterionic copolymer of acrylic acid and 3-[2-(acrylamido)-ethyl dimethylammonio] propane sulfonate (PAA-co-PAEDAPS) is resistant to cell attachment and suppresses the formation of key cytoskeletal components. Differential expression of heat shock protein 90 and actin is observed, in terms of both their magnitude and cellular localization, and distinct cytoplasmic patterns of vimentin are seen. The ionophore A23187 induces contraction in confluent hCASMC cultures on Nafion-terminated surfaces. These results demonstrate that PEMU coatings exert direct effects on the cytoskeletal organization of attaching hCASMCs, impeding growth in some cases, inducing changes consistent with phenotypic modulation in others, and suggesting potential utility for PEMU surfaces as a coating for coronary artery stents and other implantable medical devices.
Identifier: FSU_migr_chm_faculty_publications-0011 (IID), 10.4061/2011/854068 (DOI)
Citation: R.G. Newcomer, M.D. Moussallem, T.C.S. Keller, J.B. Schlenoff, Q-X A. Sang, Biotechnology Research International Volume 2011 (2011), Article ID 854068, 15 pages "Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces."
Subject(s): Biochemistry
Biotechnology
Chemistry
Links: http://dx.doi.org/10.4061/2011/854068
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_chm_faculty_publications-0011
Owner Institution: FSU
Is Part of Series: Department of Chemistry and Biochemistry Faculty Publications.
Is Part Of: Biotechnology Research International.
Issue: 2011

Choose the citation style.
Newcomer, R., Moussallem, M., Keller, T. C. S., Schlenoff, J. B., & Sang, Q. -X. (2011). Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces. Biotechnology Research International. Retrieved from http://dx.doi.org/10.4061/2011/854068