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Zymosan Fungal Infection Induces Necleosome Distributions During the Innate Immune Response on a Time Dependent Manner

Title: Zymosan Fungal Infection Induces Necleosome Distributions During the Innate Immune Response on a Time Dependent Manner.
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Name(s): Gruder, Olivia, author
Department of Biological Sciences
Type of Resource: text
Genre: Text
Issuance: serial
Date Issued: 2013
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Chromatin structure plays a critical role in the regulation of the human genome. An understanding of the role of chromatin structure and its relationship to gene regulation is critical to developing new strategies to prevent and treat diseases. We chose to investigate the anti-inflammatory response of human macrophage-like cell line (THP1) to Zymosan, in order to elucidate the regulation of chromatin. Zymosan is a component of the fungal cell wall that induces an innate immune response. After THP1 were treated with Zymosan, we hypothesized that the fungal infection would initiate an inflammatory response by altering nucleosome redistribution and/or altering chromatin structure in a time dependent manner. Based on previous results that showed rapid, widespread, transient changes in nucleosome distribution in the innate immune response, we chose to look at multiple time points at high temporal resolution: 0 (control), 20', 40', 60', 80', 100', 2h, 3h, 4h and 12h. We measured nucleosome distribution at each of these time points at hundreds of genes transcription start sites involved in the immune response. We saw the greatest changes in nucleosome positioning from 20 to 60 minutes, and it appeared that these changes were transient since they reverted back to their original after the 60-minute time point. These results support our prediction that all cells have the same nucleosome distributions during their resting states, but can be altered with the addition of an insult. In response to a stimulus, a biochemical "yawn" occurs to provide accessibility to genes needed to provide a response. The data indicates that widespread but transient changes occur to the entire genome upon response to an environmental stimulus.
Identifier: FSU_migr_undergradresearch-0006 (IID)
Subject(s): Biology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_undergradresearch-0006
Host Institution: FSU
Is Part of Series: Showcase of Undergraduate Research Excellence.

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Gruder, O. (2013). Zymosan Fungal Infection Induces Necleosome Distributions During the Innate Immune Response on a Time Dependent Manner. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_undergradresearch-0006