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Activity-Dependent Regulation of Calcium and Ribosomes in the Chick Cochlear Nucleus
| Title: | Activity-Dependent Regulation of Calcium and Ribosomes in the Chick Cochlear Nucleus. |
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| Name(s): |
Call, Cody, author Department of Psychology |
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| Type of Resource: | text | |
| Genre: | Text | |
| Issuance: | monographic | |
| Date Issued: | 2015 | |
| Physical Form: |
computer online resource |
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| Extent: | 1 online resource | |
| Language(s): | English | |
| Abstract/Description: | Cochlea removal results in the death of 20-30% of neurons in nucleus magnocellularis (NM), a cochlear nucleus of the chick auditory system involved in the precise time-coding of acoustic signals. Within 1 hr of deafferentation, intracellular calcium concentration ([Ca2+]i) rises by up to 400% while the integrity of ribosomes begins to decline—two potentially cytotoxic events. Glutamatergic axons of the auditory nerve have been shown to maintain NM neuron health by activating group I and II metabotropic glutamate receptors (mGluRs), maintaining normal [Ca2+]i and ribosomal integrity. This study aimed to determine how [Ca2+]i and ribosomal integrity are maintained by auditory nerve stimulation by selectively blocking group I mGluRs with AIDA and group II mGluRs with LY 341495 during unilateral auditory nerve stimulation. The abundance of Ca2+ in NM neurons was quantified using in vitro fura-2 ratiometric calcium imaging, while ribosomal integrity was assayed in a subset of the same tissue slices using Y10B immunolabeling (Y10B-ir). It was expected that AIDA and LY 341495 would increase [Ca2+]i and these increases would occur in parallel with an elimination in stimulation-induced differences in Y10B-ir between stimulated and unstimulated neurons of a slice. AIDA caused large increases in [Ca2+]i and eliminated differences in Y10B-ir between sides. Surprisingly, LY 341495 failed to cause reliable increases in [Ca2+]i compared to stimulated controls, but still eliminated differences in Y10B-ir between sides. These results suggest dissociation in how calcium and ribosomes are regulated in NM neurons. | |
| Identifier: | FSU_migr_uhm-0509 (IID) | |
| Keywords: | activity-dependent, group I mGluR, group II mGluR, ribosome, calcium | |
| Submitted Note: | A Thesis submitted to the Department of Psychology in partial fulfillment of the requirements for graduation with Honors in the Major. | |
| Degree Awarded: | Spring Semester, 2015. | |
| Date of Defense: | April 9, 2015. | |
| Subject(s): |
Brain chemistry Neurochemistry Neurosciences |
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| Persistent Link to This Record: | http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0509 | |
| Owner Institution: | FSU | |
| Is Part of Series: | Honors Theses. |
Call, C. (2015). Activity-Dependent Regulation of Calcium and Ribosomes in the Chick Cochlear Nucleus. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_uhm-0509
