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Comparison of whole-genome DNA methylation patterns in whole blood, saliva, and lymphoblastoid cell lines.

Title: Comparison of whole-genome DNA methylation patterns in whole blood, saliva, and lymphoblastoid cell lines.
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Name(s): Thompson, Tara M, author
Sharfi, Duaa, author
Lee, Maria, author
Yrigollen, Carolyn M, author
Naumova, Oksana Yu, author
Grigorenko, Elena L, author
Type of Resource: text
Genre: Text
Date Issued: 2013-03-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Epigenetic mechanisms, including DNA methylation, that underlie neuropsychiatric conditions have become a promising area of research. Most commonly used DNA sources in such studies are peripheral (whole) blood (WB), saliva (SL), and lymphoblastoid cell lines (LCLs); thus, the question of the consistency of DNA methylation patterns in those cells is of particular interest. To investigate this question we performed comparative analyses of methylation patterns in WB, SL, and LCLs derived from the same individuals, using Illumina HumanMethylation27 BeadChip arrays. Our results showed that DNA methylation patterns in SL are relatively consistent with those in WB, whereas the patterns in LCLs are similarly distinct from both WB and SL. The results indicated that due to multiple random and directed changes in DNA methylation throughout cell culturing, LCLs are not a reliable source of DNA for epigenetic studies and should be used with caution when investigating epigenetic mechanisms underlying biological processes.
Identifier: FSU_pmch_23269419 (IID), 10.1007/s10519-012-9579-1 (DOI), PMC3577999 (PMCID), 23269419 (RID), 23269419 (EID)
Grant Number: DC007665, P50 HD052120, P50 HD052120, R01 DC007665, R25 HL088730, R25HL088730
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577999.
Subject(s): Adolescent
Adult
Blood Chemical Analysis/methods
Cell Line
Child
DNA Methylation
Epigenesis, Genetic
Female
Gene Expression Profiling/methods
Humans
Lymphocytes
Male
Middle Aged
Saliva/chemistry
Young Adult
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_23269419
Owner Institution: FSU
Is Part Of: Behavior genetics.
1573-3297
Issue: iss. 2, vol. 43

Choose the citation style.
Thompson, T. M., Sharfi, D., Lee, M., Yrigollen, C. M., Naumova, O. Y., & Grigorenko, E. L. (2013). Comparison of whole-genome DNA methylation patterns in whole blood, saliva, and lymphoblastoid cell lines. Behavior Genetics. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_23269419