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Rapid detection of bovine adipose tissue using lateral flow strip assay

Title: Rapid Detection Of Bovine Adipose Tissue Using Lateral Flow Strip Assay.
Name(s): Hsieh, Yun-Hwa P., author
Gajewski, Kamil, author
Type of Resource: text
Genre: Text
Date Issued: 2016-07
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Currently no rapid immunoassays are developed to identify the species content of fat tissue in mixtures. We report a simple protocol enabling the effective detection of bovine fat in highly processed materials using a lateral flow (LF) immunoassay which targets a ruminant-specific muscle protein. A portion (50 gm) of muscle-free fat samples was rendered to separate the molten fat from the proteinaceous residue, then soluble proteins were extracted from the solid residue with 0.5 mol/L NaCl for the LF analysis. The assay could detect 2% bovine fat-in-pork fat, 1% bovine fat-in-porcine meat-and-bone meal, and 0.5% bovine fat-in-soy meal mixtures. Rendered bovine fat could be detected up to 213 degrees C. These results demonstrate that low levels of bovine fat tissue can be detected in processed materials using an immunoassay based on the presence of the muscle protein which serves as a species marker in the fat tissue.
Identifier: FSU_libsubv1_wos_000382728500009 (IID), 10.1002/fsn3.322 (DOI)
Keywords: Adulteration, animal fats, authentication, differentiation, identification, immunoassay, lard, lipids, oils, origins, pcr, products, proteins, raman-spectroscopy
Publication Note: The publisher’s version of record is available at
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Host Institution: FSU
Is Part Of: Food Science & Nutrition.
Issue: iss. 4, vol. 4

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Hsieh, Y. -H. P., & Gajewski, K. (2016). Rapid Detection Of Bovine Adipose Tissue Using Lateral Flow Strip Assay. Food Science & Nutrition. Retrieved from