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Notch Overexpression During Epithelial Tumorigenesis: The Role Of NICD As An Oncogene In An Lgl Knockdown System

Title: Notch Overexpression During Epithelial Tumorigenesis: The Role Of NICD As An Oncogene In An Lgl Knockdown System.
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Name(s): Tremblay, Nicholas, author
Type of Resource: text
Genre: text
bachelor thesis
Date Issued: 2017-07-27
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The Notch signaling pathway is an evolutionarily conserved method of cellular communication which coordinates cell differentiation and cell fate determination events throughout many different tissues in all metazoans. While Notch is classically referred to as a tumor suppressor gene, cases of oncogenic behavior have also been documented; this creates uncertainty regarding the pathway’s role in a tumor under tissue specific conditions. My project utilized follicle cells within the ovaries of Drosophila melanogaster as a model system to examine upregulation of Notch signaling in an epithelial cell tumor environment (generated by knockdown of lgl: a well-established tumor suppressor). While overexpression of Notch is not sufficient for tumor formation, I found that there is a significant increase in tumor area when overexpressed in Lgl knockdown multilayered follicle cells. These tumor tissues were then allografted into adult wildtype females and found to survive and grow over the course of 9 days. These results suggest a novel tumorigenic role of overactive Notch signaling when the tumor suppressive function of lgl is lost, and raise questions regarding which specific interactions were responsible for the observed incidence of follicle cell proliferation in this system.
Identifier: FSU_libsubv1_scholarship_submission_1501199321_cdb04b9d (IID)
Keywords: Drosophila, cancer, tumor, signaling, cell, polarity, expression, gene, epithelial,Oncogene
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1501199321_cdb04b9d
Owner Institution: FSU

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Tremblay, N. (2017). Notch Overexpression During Epithelial Tumorigenesis: The Role Of NICD As An Oncogene In An Lgl Knockdown System. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1501199321_cdb04b9d