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Effects of genes involving cell shape change on tumorigenesis in the salivary gland imaginal ring of Drosophila melanogaster

Title: Effects of genes involving cell shape change on tumorigenesis in the salivary gland imaginal ring of Drosophila melanogaster.
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Inaccessible until Dec 15, 2019 due to copyright restrictions.

Name(s): Mihailovic, Sonja, author
Type of Resource: text
Genre: Text
Bachelor Thesis
Date Issued: 2017-12-06
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Drosophila melanogaster have been used as a model organism for cancer because it contains genes homologous to those of humans that are involved in various cancer pathways. It has been observed that an overexpression of the Notch intracellular domain (NICD) gene of the Notch pathway, which is involved in cellular differentiation, proliferation, and development, in the salivary gland imaginal ring in 3rd instar larvae causes tumor formation in the apical area of the posterior cells. Since changes in cell-to-cell adhesion and cytoskeletal structure have been known to alter cell shape and lead to tumor formation, genes regulating these factors are possible candidates for causing tumorigenesis in the salivary gland imaginal ring. In this study, the cell size, location, and cytoskeletal structure between the posterior and anterior cells of the imaginal ring under single knock downs of the ephrin receptor (eph), rhomboid (rho), rho guanine nucleotide exchange factor 4 (rhogef4), polyhomeotic-proximal (ph-p), and ribbon (rib) genes were observed. It was found that only rib had a significantly abnormal cell shape change where the actin filaments exhibited a wave-like structure and some of the cells were in closer proximity to one another. This could be because the rib gene functions to constrict the apical side of the salivary gland during tube formation and elongation. A knock down could, therefore, result in loose actin filaments which could lead to weaker boundaries between the cells, allowing the cells to move and overlap with one another. In order to detect if post-endocycle mitosis of the imaginal ring posterior cells cause aneuploidy which could aid in the tumor formation created by Notch overexpression, the salivary gland was hybridized with a pUAS-EGFP vector to test whether a fluorescence in situ hybridization procedure customized for the salivary gland tissue would yield successful results. There was partial success in hybridization and many possible optimizations were identified.
Identifier: FSU_libsubv1_scholarship_submission_1512575130_0392ea3e (IID)
Keywords: Drosophila, tumorigenesis, cancer
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1512575130_0392ea3e
Owner Institution: FSU
Is Part Of: Effects of genes involving cell shape change on tumorigenesis in the salivary gland imaginal ring of Drosophila melanogaster.

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Mihailovic, S. (2017). Effects of genes involving cell shape change on tumorigenesis in the salivary gland imaginal ring of Drosophila melanogaster. Effects Of Genes Involving Cell Shape Change On Tumorigenesis In The Salivary Gland Imaginal Ring Of Drosophila Melanogaster. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1512575130_0392ea3e