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Comparison Of Potassium And Sodium Binding In Vivo And In Agarose Samples Using Tqtppi Pulse Sequence

Title: Comparison Of Potassium And Sodium Binding In Vivo And In Agarose Samples Using Tqtppi Pulse Sequence.
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Name(s): Schepkin, Victor D., author
Neubauer, Andreas, author
Nagel, Armin M., author
Budinger, Thomas F., author
Type of Resource: text
Genre: Journal Article
Text
Journal Article
Date Issued: 2017-04
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Potassium and sodium specific binding in vivo were explored at 21.1 T by triple quantum (TQ) magnetic resonance (MR) signals without filtration to achieve high sensitivities and precise quantifications. The pulse sequence used time proportional phase increments (TPPI). During simultaneous phase-time increments, it provided total single quantum (SQ) and TQ MR signals in the second dimension at single and triple quantum frequencies, respectively. The detection of both TQ and SQ signals was performed at identical experimental conditions and the resulting TQ signal equals 60 +/- 3% of the SQ signal when all ions experience sufficient time for binding. In a rat head in vivo the TQ percentage relative to SQ for potassium is 41.5 +/- 3% and for sodium is 16.1 +/- 1%. These percentages were compared to the matching values in an agarose tissue model with MR relaxation times similar to those of mammalian brain tissue. The sodium TQ signal in agarose samples decreased in the presence of potassium, suggesting a competitive binding of potassium relative to sodium ions for the same binding sites. The TQTPPI signals correspond to almost two times more effective binding of potassium than sodium. In vivo, up to 69% of total potassium and similar to 27% of total sodium can be regarded as bound or experiencing an association time in the range of several milliseconds. Experimental data analyses show that more than half of the in vivo total sodium TQ signal could be from extracellular space, which is an important factor for quantification of intracellular MR signals. (C) 2017 Elsevier Inc. All rights reserved.
Identifier: FSU_libsubv1_wos_000399861500021 (IID), 10.1016/j.jmr.2017.03.003 (DOI)
Keywords: human brain, relaxation, Binding, energy-metabolism, extracellular-sodium, filtered na-23 nmr, In vivo, intracellular sodium, ion homeostasis, na+ binding, perfused rat-heart, Potassium, Rat, residual quadrupolar interaction, Sodium, tqtppi, Triple quantum NMR
Publication Note: The publisher's version of record is available at https://doi.org/10.1016/j.jmr.2017.03.003
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000399861500021
Host Institution: FSU
Is Part Of: Journal of Magnetic Resonance.
1090-7807
Issue: vol. 277

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Schepkin, V. D., Neubauer, A., Nagel, A. M., & Budinger, T. F. (2017). Comparison Of Potassium And Sodium Binding In Vivo And In Agarose Samples Using Tqtppi Pulse Sequence. Journal Of Magnetic Resonance. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000399861500021