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Transcriptome analysis of flood-induced hypoxia in maize (Zea mays) seedling shoots

Title: Transcriptome analysis of flood-induced hypoxia in maize (Zea mays) seedling shoots.
Name(s): Hagemeyer, Christopher, author
Type of Resource: text
Genre: Text
Bachelor Thesis
Date Issued: 2018-08-07
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Hypoxia has been known to influence drastic changes in an organism at a molecular level. New studies are constantly unveiling new information about how organisms respond to hypoxic condition, but the mechanism in which these organisms do so is complex and remains undetermined. In this study, a full transcriptome analysis of maize (Zea mays) apical meristem tissue was conducted under hypoxic conditions by utilizing RNA-seq. The transcriptomic data received from various time points over the duration of 18 hours of hypoxia provided valuable information about the transcriptional response of hypoxic responsive genes over time. These data prompted a deeper probe into the short-term response at the 2 hour mark. An additional RNA-seq protocol was carried out after 2 hours of flood-induced hypoxia. Intensified expression levels were found in the known hypoxic responsive genes pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH). Induced transcription was observed in the rice (Oryza sativa japonica) ortholog (Zm00001d023859), which is known for ethylene-induced aerenchyma formation in roots under oxygen deficient conditions in rice. Moreover the rice ortholog (Zm00001d022084) also displayed up-regulation in gene expression compared to the control. This gene is implicated flooding tolerance during seed germination and early seedling growth in rice but is minimally annotated in maize. Induction was achieved in other hypoxia responsive genes, but not nearly to the same degree. It was thus concluded that 2 hours is an important transition period for the inbred maize line B73 to reach optimal response to hypoxia. It is hoped that this data can be used to unveil the chromatin dynamics behind this hypoxic stress response.
Identifier: FSU_libsubv1_scholarship_submission_1533651783_b354e488 (IID)
Keywords: hypoxia, maize, flood-induced, flooding, corn, transcriptome, G4, 8-oxogaunine, 8-oxog
Grant Number: Project IOS 1444532
Persistent Link to This Record:
Use and Reproduction: Creative Commons Attribution (CC BY 4.0)
Owner Institution: FSU

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Hagemeyer, C. (2018). Transcriptome analysis of flood-induced hypoxia in maize (Zea mays) seedling shoots. Retrieved from