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Comparative Analysis of Kaposi's Sarcoma-Associated Herpesvirus ORF45 Homologs

Title: A Comparative Analysis of Kaposi's Sarcoma-Associated Herpesvirus ORF45 Homologs.
Name(s): Dang, Carolyn Kellie, author
Type of Resource: text
Genre: Text
Bachelor Thesis
Date Issued: 2018-08-03
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Kaposi sarcoma is a type of lymphatic and skin cancer that is caused by Kaposi Sarcoma-associated virus (KSHV). KSHV ORF45 is classified as an immediate early gene that has multifunctional roles. One of its major functions is to activate the extracellular signal-regulated kinase (ERK) and p90 ribosomal kinase 1 and 2 (RSK1/2) in the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway. It has been discovered that KSHV ORF45 can activate both the ERK and RSK which promote the reactivation of KSHV. There is limited evidence that observes the behavior of all KSHV ORF45 homologs. Therefore, we are interested in studying the functional conservativeness within gammaherpesvirus ORF45 subfamily, including the ability of ORF45 homologues to activate ERK and RSK, and the colonigenesis of HeLa cells transfected with ORF45 homologues. In this study, we found that amongst all ORF45 homologues, there is strong conservation in the amino-terminus, ERK, RSK and ORF33 binding sites. We examined that there are different levels of phosphorylated RSK and ERK amongst the gammaherpesvirus homologues. In addition, we examined that G418 selection of different ORF45 homologues. The result showed no significant difference between the gammaherpesvirus-2 subgroups (Rhadinovirus, Macavirus, and Percavirus).
Identifier: FSU_libsubv1_scholarship_submission_1533768242_bc8f99d4 (IID)
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Owner Institution: FSU

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Dang, C. K. (2018). A Comparative Analysis of Kaposi's Sarcoma-Associated Herpesvirus ORF45 Homologs. Retrieved from