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Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype.
| Title: | Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype. |
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| Name(s): |
Rao, Anand N, author Falnikar, Aditi, author O'Toole, Eileen T, author Morphew, Mary K, author Hoenger, Andreas, author Davidson, Michael W, author Yuan, Xiaobing, author Baas, Peter W, author |
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| Type of Resource: | text | |
| Genre: |
Journal Article Text |
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| Date Issued: | 2016-05-09 | |
| Physical Form: |
computer online resource |
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| Extent: | 1 online resource | |
| Language(s): | English | |
| Abstract/Description: | Contemporary models for neuronal migration are grounded in the view that virtually all functionally relevant microtubules (MTs) in migrating neurons are attached to the centrosome, which occupies a position between the nucleus and a short leading process. It is assumed that MTs do not undergo independent movements but rather transduce forces that enable movements of the centrosome and nucleus. The present results demonstrate that although this is mostly true, a small fraction of the MTs are centrosome-unattached, and this permits limited sliding of MTs. When this sliding is pharmacologically inhibited, the leading process becomes shorter, migration of the neuron deviates from its normal path, and the MTs within the leading process become buckled. Partial depletion of ninein, a protein that attaches MTs to the centrosome, leads to greater numbers of centrosome-unattached MTs as well as greater sliding of MTs. Concomitantly, the soma becomes less mobile and the leading process acquires an elongated morphology akin to an axon. | |
| Identifier: | FSU_pmch_27138250 (IID), 10.1083/jcb.201506140 (DOI), PMC4862329 (PMCID), 27138250 (RID), 27138250 (EID), jcb.201506140 (PII) | |
| Grant Number: | F31 NS093748, P41 GM103431, R01 NS028785, R56 NS028785 | |
| Publication Note: | This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4862329. | |
| Subject(s): |
Animals Cell Movement/physiology Centrosome/metabolism Centrosome/ultrastructure Cytoskeletal Proteins/genetics Cytoskeletal Proteins/metabolism Cytoskeletal Proteins/physiology Microscopy, Electron, Transmission Microtubule-Associated Proteins/genetics Microtubule-Associated Proteins/metabolism Microtubule-Associated Proteins/physiology Microtubules/metabolism Microtubules/ultrastructure Neurons/metabolism Neurons/physiology Neurons/ultrastructure Nuclear Proteins/genetics Nuclear Proteins/metabolism Nuclear Proteins/physiology Phenotype RNA Interference Rats |
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| Persistent Link to This Record: | http://purl.flvc.org/fsu/fd/FSU_pmch_27138250 | |
| Host Institution: | FSU | |
| Is Part Of: |
The Journal of cell biology. 1540-8140 |
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| Issue: | iss. 3, vol. 213 |
Rao, A. N., Falnikar, A., O'Toole, E. T., Morphew, M. K., Hoenger, A., Davidson, M. W., … Baas, P. W. (2016). Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype. The Journal Of Cell Biology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27138250
