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Anchored enrichment dataset for true flies (order Diptera) reveals insights into the phylogeny of flower flies (family Syrphidae).

Title: Anchored enrichment dataset for true flies (order Diptera) reveals insights into the phylogeny of flower flies (family Syrphidae).
Name(s): Young, Andrew Donovan, author
Lemmon, Alan R, author
Skevington, Jeffrey H, author
Mengual, Ximo, author
Ståhls, Gunilla, author
Reemer, Menno, author
Jordaens, Kurt, author
Kelso, Scott, author
Lemmon, Emily Moriarty, author
Hauser, Martin, author
De Meyer, Marc, author
Misof, Bernhard, author
Wiegmann, Brian M, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2016-06-29
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Anchored hybrid enrichment is a form of next-generation sequencing that uses oligonucleotide probes to target conserved regions of the genome flanked by less conserved regions in order to acquire data useful for phylogenetic inference from a broad range of taxa. Once a probe kit is developed, anchored hybrid enrichment is superior to traditional PCR-based Sanger sequencing in terms of both the amount of genomic data that can be recovered and effective cost. Due to their incredibly diverse nature, importance as pollinators, and historical instability with regard to subfamilial and tribal classification, Syrphidae (flower flies or hoverflies) are an ideal candidate for anchored hybrid enrichment-based phylogenetics, especially since recent molecular phylogenies of the syrphids using only a few markers have resulted in highly unresolved topologies. Over 6200 syrphids are currently known and uncovering their phylogeny will help us to understand how these species have diversified, providing insight into an array of ecological processes, from the development of adult mimicry, the origin of adult migration, to pollination patterns and the evolution of larval resource utilization. We present the first use of anchored hybrid enrichment in insect phylogenetics on a dataset containing 30 flower fly species from across all four subfamilies and 11 tribes out of 15. To produce a phylogenetic hypothesis, 559 loci were sampled to produce a final dataset containing 217,702 sites. We recovered a well resolved topology with bootstrap support values that were almost universally >95 %. The subfamily Eristalinae is recovered as paraphyletic, with the strongest support for this hypothesis to date. The ant predators in the Microdontinae are sister to all other syrphids. Syrphinae and Pipizinae are monophyletic and sister to each other. Larval predation on soft-bodied hemipterans evolved only once in this family. Anchored hybrid enrichment was successful in producing a robustly supported phylogenetic hypothesis for the syrphids. Subfamilial reconstruction is concordant with recent phylogenetic hypotheses, but with much higher support values. With the newly designed probe kit this analysis could be rapidly expanded with further sampling, opening the door to more comprehensive analyses targeting problem areas in syrphid phylogenetics and ecology.
Identifier: FSU_pmch_27357120 (IID), 10.1186/s12862-016-0714-0 (DOI), PMC4928351 (PMCID), 27357120 (RID), 27357120 (EID), 10.1186/s12862-016-0714-0 (PII)
Keywords: Anchored phylogenetics, Eristalinae, Flower flies, Hoverflies, Hybrid enrichment, Microdontinae, Pipizinae, Syrphinae
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Animals
Biological Evolution
High-Throughput Nucleotide Sequencing
Sequence Analysis, DNA
Persistent Link to This Record:
Host Institution: FSU
Is Part Of: BMC evolutionary biology.
Issue: iss. 1, vol. 16

Choose the citation style.
Young, A. D., Lemmon, A. R., Skevington, J. H., Mengual, X., Ståhls, G., Reemer, M., … Wiegmann, B. M. (2016). Anchored enrichment dataset for true flies (order Diptera) reveals insights into the phylogeny of flower flies (family Syrphidae). Bmc Evolutionary Biology. Retrieved from