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mA pathway facilitates sex determination in Drosophila.

Title: The mA pathway facilitates sex determination in Drosophila.
Name(s): Kan, Lijuan, author
Grozhik, Anya V, author
Vedanayagam, Jeffrey, author
Patil, Deepak P, author
Pang, Nan, author
Lim, Kok-Seong, author
Huang, Yi-Chun, author
Joseph, Brian, author
Lin, Ching-Jung, author
Despic, Vladimir, author
Guo, Jian, author
Yan, Dong, author
Kondo, Shu, author
Deng, Wu-Min, author
Dedon, Peter C, author
Jaffrey, Samie R, author
Lai, Eric C, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2017-07-04
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The conserved modification N-methyladenosine (mA) modulates mRNA processing and activity. Here, we establish the Drosophila system to study the mA pathway. We first apply miCLIP to map mA across embryogenesis, characterize its mA 'writer' complex, validate its YTH 'readers' CG6422 and YT521-B, and generate mutants in five mA factors. While mA factors with additional roles in splicing are lethal, mA-specific mutants are viable but present certain developmental and behavioural defects. Notably, mA facilitates the master female determinant Sxl, since multiple mA components enhance female lethality in Sxl sensitized backgrounds. The mA pathway regulates Sxl processing directly, since miCLIP data reveal Sxl as a major intronic mA target, and female-specific Sxl splicing is compromised in multiple mA pathway mutants. YT521-B is a dominant mA effector for Sxl regulation, and YT521-B overexpression can induce female-specific Sxl splicing. Overall, our transcriptomic and genetic toolkit reveals in vivo biologic function for the Drosophila mA pathway.
Identifier: FSU_pmch_28675155 (IID), 10.1038/ncomms15737 (DOI), PMC5500889 (PMCID), 28675155 (RID), 28675155 (EID), ncomms15737 (PII)
Grant Number: R01 ES017010, R01 GM072562, P30 CA008748, P30 ES002109, R01 DA037150, R21 ES022858, R01 NS083833, R01 NS074037, R01 GM083300
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Adenosine/analogs & derivatives
Alternative Splicing
Amino Acid Motifs
Behavior, Animal
DNA Methylation
Drosophila Proteins/metabolism
Drosophila melanogaster/genetics
Gene Expression Regulation, Developmental
Mass Spectrometry
Models, Genetic
Multigene Family
RNA, Messenger/metabolism
RNA-Binding Proteins/metabolism
Sex Determination Processes
Persistent Link to This Record:
Owner Institution: FSU
Is Part Of: Nature communications.
Issue: vol. 8

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Kan, L., Grozhik, A. V., Vedanayagam, J., Patil, D. P., Pang, N., Lim, K. -S., … Lai, E. C. (2017). The mA pathway facilitates sex determination in Drosophila. Nature Communications. Retrieved from