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Structure of isolated Z-disks from honeybee flight muscle.

Title: Structure of isolated Z-disks from honeybee flight muscle.
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Name(s): Rusu, Mara, author
Hu, Zhongjun, author
Taylor, Kenneth A, author
Trinick, John, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-04-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The Z-disk is a complex structure comprising some 40 proteins that are involved in the transmission of force developed during muscle contraction and in important signalling pathways that govern muscle homeostasis. In the Z-disk the ends of antiparallel thin filaments from adjacent sarcomeres are crosslinked by α-actinin. The structure of the Z-disk lattice varies greatly throughout the animal kingdom. In vertebrates the thin filaments form a tetragonal lattice, whereas invertebrate flight muscle has a hexagonal lattice. The width of the Z-disk varies considerably and correlates with the number of α-actinin bridges. A detailed description at a high resolution of the Z-disk lattice is needed in order to better understand muscle function and disease. The molecular architecture of the Z-disk lattice in honeybee (Apis mellifera) is known from plastic embedded thin sections to a resolution of 7 nm, which is not sufficient to dock component protein crystal structures. It has been shown that sectioning is a damaging process that leads to the loss of finer details present in biological specimens. However, the Apis Z-disk is a thin structure (120 nm) suitable for cryo EM. We have isolated intact honeybee Z-disks from indirect flight muscle, thus obviating the need of plastic sectioning. We have employed cryo electron tomography and image processing to investigate the arrangement of proteins within the hexagonal lattice of the Apis Z-disk. The resolution obtained, ~6 nm, was probably limited by damage caused by the harshness of the conditions used to extract the myofibrils and isolate the Z-disks.
Identifier: FSU_pmch_28733815 (IID), 10.1007/s10974-017-9477-5 (DOI), PMC5660141 (PMCID), 28733815 (RID), 28733815 (EID), 10.1007/s10974-017-9477-5 (PII)
Keywords: Cryo electron tomography, Indirect flight muscle, Subtomogram averaging, Z-disk, α-actinin
Grant Number: R01 GM030598
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660141.
Subject(s): Animals
Bees
Cytoskeleton/metabolism
Muscle Contraction/physiology
Muscle Proteins/metabolism
Myofibrils/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28733815
Owner Institution: FSU
Is Part Of: Journal of muscle research and cell motility.
1573-2657
Issue: iss. 2, vol. 38

Choose the citation style.
Rusu, M., Hu, Z., Taylor, K. A., & Trinick, J. (2017). Structure of isolated Z-disks from honeybee flight muscle. Journal Of Muscle Research And Cell Motility. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28733815