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Direct detection of neural activity in vitro using magnetic resonance electrical impedance tomography (MREIT).

Title: Direct detection of neural activity in vitro using magnetic resonance electrical impedance tomography (MREIT).
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Name(s): Sadleir, Rosalind J, author
Fu, Fanrui, author
Falgas, Corey, author
Holland, Stephen, author
Boggess, May, author
Grant, Samuel C, author
Woo, Eung Je, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-11-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: We describe a sequence of experiments performed in vitro to verify the existence of a new magnetic resonance imaging contrast - Magnetic Resonance Electrical Impedance Tomography (MREIT) -sensitive to changes in active membrane conductivity. We compared standard deviations in MREIT phase data from spontaneously active Aplysia abdominal ganglia in an artificial seawater background solution (ASW) with those found after treatment with an excitotoxic solution (KCl). We found significant increases in MREIT treatment cases, compared to control ganglia subject to extra ASW. This distinction was not found in phase images from the same ganglia using no imaging current. Further, significance and effect size depended on the amplitude of MREIT imaging current used. We conclude that our observations were linked to changes in cell conductivity caused by activity. Functional MREIT may have promise as a more direct method of functional neuroimaging than existing methods that image correlates of blood flow such as BOLD fMRI.
Identifier: FSU_pmch_28818695 (IID), 10.1016/j.neuroimage.2017.08.004 (DOI), PMC5696120 (PMCID), 28818695 (RID), 28818695 (EID), S1053-8119(17)30651-1 (PII)
Keywords: Action potential, Aplysia, MREIT, MRI, Microelectrode array, FMRI
Grant Number: R01 NS077004
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696120.
Subject(s): Action Potentials/drug effects
Action Potentials/physiology
Animals
Aplysia
Butyrates/pharmacology
Electric Impedance
Ganglia, Invertebrate/diagnostic imaging
Ganglia, Invertebrate/drug effects
Hydrocarbons, Fluorinated/pharmacology
In Vitro Techniques
Magnetic Resonance Imaging/methods
Neurons/drug effects
Neurons/physiology
Neurotoxins/pharmacology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28818695
Owner Institution: FSU
Is Part Of: NeuroImage.
1095-9572
Issue: vol. 161

Choose the citation style.
Sadleir, R. J., Fu, F., Falgas, C., Holland, S., Boggess, M., Grant, S. C., & Woo, E. J. (2017). Direct detection of neural activity in vitro using magnetic resonance electrical impedance tomography (MREIT). Neuroimage. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28818695