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Title
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Studies of Mechanisms Underlying Cell Competition Induced by Imbalance of Energy Metabolism.
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Creator
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Arrington, Brooke Aileen
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Abstract/Description
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Abstract Adenosine triphosphate (ATP) is the molecular currency for cells of all living organisms. When its synthesis, through ATP Synthase, is inhibited or “knocked down” cellular metabolic pathways will be compromised and, I hypothesize that, cell competition will result. Cell competition is a method by which tissue integrity and homeostasis is maintained. When neighboring cells differ in cell fitness, the fit cells will outcompete the weakened cells, leading to cell competition, and...
Show moreAbstract Adenosine triphosphate (ATP) is the molecular currency for cells of all living organisms. When its synthesis, through ATP Synthase, is inhibited or “knocked down” cellular metabolic pathways will be compromised and, I hypothesize that, cell competition will result. Cell competition is a method by which tissue integrity and homeostasis is maintained. When neighboring cells differ in cell fitness, the fit cells will outcompete the weakened cells, leading to cell competition, and therefore, tissue homeostasis. In this experiment, I explored the consequences of depriving cells of ATP Synthase. In order to see cell competition, a mosaic condition using the GeneSwitch Gal4 system was created, consisting of wild type and ATP Synthase knockdown cells. Using the Gal80TS System, a control was also set up creating a homogenous condition of only ATP Synthase knock down cells. Cell competition was visualized using DCP-1 (death caspase-1) staining which targets apoptotic cells. Apoptosis was seen in the mosaic condition, where cells of different fitness neighbored one another. No cell competition was seen in the homogenous condition. The intracellular signaling pathways followed during cell competition were then explored, comparing the deprivation of ATP to the deprivation of insulin. tGPH, which fluoresces green under a confocal microscope, was used in order to trace levels of PI3K following ATP Synthase knock down. When cells are deprived of insulin, tGPH localization to the cell membrane decreases substantially. Similar results were seen in this experiment when cells are deprived of ATP. This result leads to the hypothesis that the insulin and ATP intracellular signaling pathways may be similar or connected. These results allow for further research in this field of interest.
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Date Issued
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2016-04-21
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Identifier
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FSU_libsubv1_scholarship_submission_1461261313
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Format
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Thesis
