Search results

Title: Practice-based Research Networks (PBRNs) Bridging the Gaps between Communities, Funders, and Policymakers.
Creator: Gaglioti, Anne H, Werner, James J, Rust, George, Fagnan, Lyle J, Neale, Anne Victoria
Abstract/Description: In this commentary, we propose that practice-based research networks (PBRNs) engage with funders and policymakers by applying the same engagement strategies they have successfully used to build relationships with community stakeholders. A community engagement approach to achieve new funding streams for PBRNs should include a strategy to engage key stakeholders from the communities of funders, thought leaders, and policymakers using collaborative principles and methods. PBRNs that implement...
Show moreIn this commentary, we propose that practice-based research networks (PBRNs) engage with funders and policymakers by applying the same engagement strategies they have successfully used to build relationships with community stakeholders. A community engagement approach to achieve new funding streams for PBRNs should include a strategy to engage key stakeholders from the communities of funders, thought leaders, and policymakers using collaborative principles and methods. PBRNs that implement this strategy would build a robust network of engaged partners at the community level, across networks, and would reach state and federal policymakers, academic family medicine departments, funding bodies, and national thought leaders in the redesign of health care delivery.
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Date Issued: 2016-09-01
Identifier: FSU_pmch_27613796, 10.3122/jabfm.2016.05.160080, PMC5030066, 27613796, 27613796, 29/5/630
Format: Citation

Title: Correction: Retinopathy and Uveitis Associated with Sofosbuvir Therapy for Chronic Hepatitis C Infection..
Creator: Chin Loy, Katrina, Galaydh, Farah, Shaikh, Saad
Abstract/Description: [This corrects the article DOI: 10.7759/cureus.597.].
Date Issued: 2016-07-14
Identifier: FSU_pmch_27610281, 10.7759/cureus.c3, PMC4999155, 27610281, 27610281
Format: Citation

Title: Structure of myosin filaments from relaxed flight muscle by cryo-EM at 6 Å resolution.
Creator: Hu, Zhongjun, Taylor, Dianne W, Reedy, Michael K, Edwards, Robert J, Taylor, Kenneth A
Abstract/Description: We describe a cryo-electron microscopy three-dimensional image reconstruction of relaxed myosin II-containing thick filaments from the flight muscle of the giant water bug . The relaxed thick filament structure is a key element of muscle physiology because it facilitates the reextension process following contraction. Conversely, the myosin heads must disrupt their relaxed arrangement to drive contraction. Previous models predicted that myosin was unique in having an intermolecular head-head...
Show moreWe describe a cryo-electron microscopy three-dimensional image reconstruction of relaxed myosin II-containing thick filaments from the flight muscle of the giant water bug . The relaxed thick filament structure is a key element of muscle physiology because it facilitates the reextension process following contraction. Conversely, the myosin heads must disrupt their relaxed arrangement to drive contraction. Previous models predicted that myosin was unique in having an intermolecular head-head interaction, as opposed to the intramolecular head-head interaction observed in all other species. In contrast to the predicted model, we find an intramolecular head-head interaction, which is similar to that of other thick filaments but oriented in a distinctly different way. The arrangement of myosin's long α-helical coiled-coil rod domain has been hypothesized as either curved layers or helical subfilaments. Our reconstruction is the first report having sufficient resolution to track the rod α helices in their native environment at resolutions ~5.5 Å, and it shows that the layer arrangement is correct for . Threading separate paths through the forest of myosin coiled coils are four nonmyosin peptides. We suggest that the unusual position of the heads and the rod arrangement separated by nonmyosin peptides are adaptations for mechanical signal transduction whereby applied tension disrupts the myosin heads as a component of stretch activation.
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Date Issued: 2016-09-30
Identifier: FSU_pmch_27704041, 10.1126/sciadv.1600058, PMC5045269, 27704041, 27704041, 1600058
Format: Citation

Title: A route for a strong increase of critical current in nanostrained iron-based superconductors.
Creator: Ozaki, Toshinori, Wu, Lijun, Zhang, Cheng, Jaroszynski, Jan, Si, Weidong, Zhou, Juan, Zhu, Yimei, Li, Qiang
Abstract/Description: The critical temperature T and the critical current density J determine the limits to large-scale superconductor applications. Superconductivity emerges at T. The practical current-carrying capability, measured by J, is the ability of defects in superconductors to pin the magnetic vortices, and that may reduce T. Simultaneous increase of T and J in superconductors is desirable but very difficult to realize. Here we demonstrate a route to raise both T and J together in iron-based...
Show moreThe critical temperature T and the critical current density J determine the limits to large-scale superconductor applications. Superconductivity emerges at T. The practical current-carrying capability, measured by J, is the ability of defects in superconductors to pin the magnetic vortices, and that may reduce T. Simultaneous increase of T and J in superconductors is desirable but very difficult to realize. Here we demonstrate a route to raise both T and J together in iron-based superconductors. By using low-energy proton irradiation, we create cascade defects in FeSeTe films. T is enhanced due to the nanoscale compressive strain and proximity effect, whereas J is doubled under zero field at 4.2 K through strong vortex pinning by the cascade defects and surrounding nanoscale strain. At 12 K and above 15 T, one order of magnitude of J enhancement is achieved in both parallel and perpendicular magnetic fields to the film surface.
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Date Issued: 2016-10-06
Identifier: FSU_pmch_27708268, 10.1038/ncomms13036, PMC5059717, 27708268, 27708268, ncomms13036
Format: Citation

Title: Risk Factors for Substance Misuse and Adolescents' Symptoms of Depression.
Creator: Siennick, Sonja E, Widdowson, Alex O, Woessner, Mathew K, Feinberg, Mark E, Spoth, Richard L
Abstract/Description: Depressive symptoms during adolescence are positively associated with peer-related beliefs, perceptions, and experiences that are known risk factors for substance misuse. These same risk factors are targeted by many universal substance misuse prevention programs. This study examined whether a multicomponent universal substance misuse intervention for middle schoolers reduced the associations between depressive symptoms, these risk factors, and substance misuse. The study used data from a...
Show moreDepressive symptoms during adolescence are positively associated with peer-related beliefs, perceptions, and experiences that are known risk factors for substance misuse. These same risk factors are targeted by many universal substance misuse prevention programs. This study examined whether a multicomponent universal substance misuse intervention for middle schoolers reduced the associations between depressive symptoms, these risk factors, and substance misuse. The study used data from a place-randomized trial of the Promoting School-Community-University Partnerships to Enhance Resilience model for delivery of evidence-based substance misuse programs for middle schoolers. Three-level within-person regression models were applied to four waves of survey, and social network data from 636 adolescents followed from sixth through ninth grades. When adolescents in control school districts had more symptoms of depression, they believed more strongly that substance use had social benefits, perceived higher levels of substance misuse among their peers and friends, and had more friends who misused substances, although they were not more likely to use substances themselves. Many of the positive associations of depressive symptoms with peer-related risk factors were significantly weaker or not present among adolescents in intervention school districts. The Promoting School-Community-University Partnerships to Enhance Resilience interventions reduced the positive associations of adolescent symptoms of depression with peer-related risk factors for substance misuse.
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Date Issued: 2017-01-01
Identifier: FSU_pmch_27751712, 10.1016/j.jadohealth.2016.08.010, PMC5182119, 27751712, 27751712, S1054-139X(16)30254-3
Format: Citation

Title: An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation.
Creator: Perez-Arnaiz, Patricia, Kaplan, Daniel L
Abstract/Description: Mcm10 is an essential protein that functions to initiate DNA replication after the formation of the replication fork helicase. In this manuscript, we identified a budding yeast Mcm10 mutant (Mcm10-m2,3,4) that is defective in DNA binding in vitro. Moreover, this Mcm10-m2,3,4 mutant does not stimulate the phosphorylation of Mcm2 by Dbf4-dependent kinase (DDK) in vitro. When we expressed wild-type levels of mcm10-m2,3,4 in budding yeast cells, we observed a severe growth defect and a...
Show moreMcm10 is an essential protein that functions to initiate DNA replication after the formation of the replication fork helicase. In this manuscript, we identified a budding yeast Mcm10 mutant (Mcm10-m2,3,4) that is defective in DNA binding in vitro. Moreover, this Mcm10-m2,3,4 mutant does not stimulate the phosphorylation of Mcm2 by Dbf4-dependent kinase (DDK) in vitro. When we expressed wild-type levels of mcm10-m2,3,4 in budding yeast cells, we observed a severe growth defect and a substantially decreased DNA replication. We also observed a substantially reduced replication protein A- chromatin immunoprecipitation signal at origins of replication, reduced levels of DDK-phosphorylated Mcm2, and diminished Go, Ichi, Ni, and San (GINS) association with Mcm2-7 in vivo. mcm5-bob1 bypasses the growth defect conferred by DDK-phosphodead Mcm2 in budding yeast. However, the growth defect observed by expressing mcm10-m2,3,4 is not bypassed by the mcm5-bob1 mutation. Furthermore, origin melting and GINS association with Mcm2-7 are substantially decreased for cells expressing mcm10-m2,3,4 in the mcm5-bob1 background. Thus, the origin melting and GINS-Mcm2-7 interaction defects we observed for mcm10-m2,3,4 are not explained by decreased Mcm2 phosphorylation by DDK, since the defects persist in an mcm5-bob1 background. These data suggest that DNA binding by Mcm10 is essential for the initiation of DNA replication.
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Date Issued: 2016-11-20
Identifier: FSU_pmch_27751725, 10.1016/j.jmb.2016.10.014, PMC5115986, 27751725, 27751725, S0022-2836(16)30429-6
Format: Citation

Title: Acute Sleep Deprivation Blocks Short- and Long-Term Operant Memory in .
Creator: Krishnan, Harini C, Gandour, Catherine E, Ramos, Joshua L, Wrinkle, Mariah C, Sanchez-Pacheco, Joseph J, Lyons, Lisa C
Abstract/Description: Insufficient sleep in individuals appears increasingly common due to the demands of modern work schedules and technology use. Consequently, there is a growing need to understand the interactions between sleep deprivation and memory. The current study determined the effects of acute sleep deprivation on short and long-term associative memory using the marine mollusk , a relatively simple model system well known for studies of learning and memory. were sleep deprived for 9 hours using context...
Show moreInsufficient sleep in individuals appears increasingly common due to the demands of modern work schedules and technology use. Consequently, there is a growing need to understand the interactions between sleep deprivation and memory. The current study determined the effects of acute sleep deprivation on short and long-term associative memory using the marine mollusk , a relatively simple model system well known for studies of learning and memory. were sleep deprived for 9 hours using context changes and tactile stimulation either prior to or after training for the operant learning paradigm, learning that food is inedible (LFI). The effects of sleep deprivation on short-term (STM) and long-term memory (LTM) were assessed. Acute sleep deprivation prior to LFI training impaired the induction of STM and LTM with persistent effects lasting at least 24 h. Sleep deprivation immediately after training blocked the consolidation of LTM. However, sleep deprivation following the period of molecular consolidation did not affect memory recall. Memory impairments were independent of handling-induced stress, as daytime handled control animals demonstrated no memory deficits. Additional training immediately after sleep deprivation failed to rescue the induction of memory, but additional training alleviated the persistent impairment in memory induction when training occurred 24 h following sleep deprivation. Acute sleep deprivation inhibited the induction and consolidation, but not the recall of memory. These behavioral studies establish as an effective model system for studying the interactions between sleep and memory formation.
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Date Issued: 2016-12-01
Identifier: FSU_pmch_27748243, 10.5665/sleep.6320, PMC5103805, 27748243, 27748243, sp-00313-16
Format: Citation

Title: Perceived weight discrimination mediates the prospective relation between obesity and depressive symptoms in U.S. and U.K. adults.
Creator: Robinson, Eric, Sutin, Angelina, Daly, Michael
Abstract/Description: Obesity has been shown to increase risk of depression. Persons with obesity experience discrimination because of their body weight. Across 3 studies, we tested for the first time whether experiencing (perceived) weight-based discrimination explains why obesity is prospectively associated with increases in depressive symptoms. Data from 3 studies, including the English Longitudinal Study of Ageing (2008/2009-2012/2013), the Health and Retirement Study (2006/2008-2010/2012), and Midlife in the...
Show moreObesity has been shown to increase risk of depression. Persons with obesity experience discrimination because of their body weight. Across 3 studies, we tested for the first time whether experiencing (perceived) weight-based discrimination explains why obesity is prospectively associated with increases in depressive symptoms. Data from 3 studies, including the English Longitudinal Study of Ageing (2008/2009-2012/2013), the Health and Retirement Study (2006/2008-2010/2012), and Midlife in the United States (1995/1996-2004/2005), were used to examine associations between obesity, perceived weight discrimination, and depressive symptoms among 20,286 U.S. and U.K. adults. Across all 3 studies, Class II and III obesity were reliably associated with increases in depressive symptoms from baseline to follow-up. Perceived weight-based discrimination predicted increases in depressive symptoms over time and mediated the prospective association between obesity and depressive symptoms in all 3 studies. Persons with Class II and III obesity were more likely to report experiencing weight-based discrimination, and this explained approximately 31% of the obesity-related increase in depressive symptoms on average across the 3 studies. In U.S. and U.K. samples, the prospective association between obesity (defined using body mass index) and increases in depressive symptoms in adulthood may in part be explained by perceived weight discrimination. (PsycINFO Database Record
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Date Issued: 2017-02-01
Identifier: FSU_pmch_27748611, 10.1037/hea0000426, PMC5267562, 27748611, 27748611, 2016-49908-001
Format: Citation

Title: Coordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment..
Creator: Zou, Peng, Liu, Longhua, Zheng, Louise D, Payne, Kyle K, Manjili, Masoud H, Idowu, Michael O, Zhang, Jinfeng, Schmelz, Eva M, Cheng, Zhiyong
Abstract/Description: Overactive mitochondrial fission was shown to promote cell transformation and tumor growth. It remains elusive how mitochondrial quality is regulated in such conditions. Here, we show that upregulation of mitochondrial fission protein, dynamin related protein-1 (Drp1), was accompanied with increased mitochondrial biogenesis markers (PGC1, NRF1, and Tfam) in breast cancer cells. However, mitochondrial number was reduced, which was associated with lower mitochondrial oxidative capacity in...
Show moreOveractive mitochondrial fission was shown to promote cell transformation and tumor growth. It remains elusive how mitochondrial quality is regulated in such conditions. Here, we show that upregulation of mitochondrial fission protein, dynamin related protein-1 (Drp1), was accompanied with increased mitochondrial biogenesis markers (PGC1, NRF1, and Tfam) in breast cancer cells. However, mitochondrial number was reduced, which was associated with lower mitochondrial oxidative capacity in breast cancer cells. This contrast might be owing to enhanced mitochondrial turnover through autophagy, because an increased population of autophagic vacuoles engulfing mitochondria was observed in the cancer cells. Consistently, BNIP3 (a mitochondrial autophagy marker) and autophagic flux were significantly upregulated, indicative of augmented mitochondrial autophagy (mitophagy). The upregulation of Drp1 and BNIP3 was also observed in vivo (human breast carcinomas). Importantly, inhibition of Drp1 significantly suppressed mitochondrial autophagy, metabolic reprogramming, and cancer cell viability. Together, this study reveals coordinated increase of mitochondrial biogenesis and mitophagy in which Drp1 plays a central role regulating breast cancer cell metabolism and survival. Given the emerging evidence of PGC1 contributing to tumor growth, it will be of critical importance to target both mitochondrial biogenesis and mitophagy for effective cancer therapeutics.
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Date Issued: 2016-01-01
Identifier: FSU_pmch_27746856, 10.1155/2016/4085727, PMC5056295, 27746856, 27746856
Format: Citation

Title: Differential Effects of Estrogen and Progesterone on Genetic and Environmental Risk for Emotional Eating in Women.
Creator: Klump, Kelly L, O'Connor, Shannon M, Hildebrandt, Britny A, Keel, Pamela K, Neale, Michael, Sisk, Cheryl L, Boker, Steven, Burt, S Alexandra
Abstract/Description: Recent data show shifts in genetic and environmental influences on emotional eating across the menstrual cycle, with significant shared environmental influences during pre-ovulation, and primarily genetic effects during post-ovulation. Factors driving differential effects are unknown, although increased estradiol during pre-ovulation and increased progesterone during post-ovulation are thought to play a role. We indirectly investigated this possibility by examining whether overall levels of...
Show moreRecent data show shifts in genetic and environmental influences on emotional eating across the menstrual cycle, with significant shared environmental influences during pre-ovulation, and primarily genetic effects during post-ovulation. Factors driving differential effects are unknown, although increased estradiol during pre-ovulation and increased progesterone during post-ovulation are thought to play a role. We indirectly investigated this possibility by examining whether overall levels of estradiol and progesterone differentially impact genetic and environmental risk for emotional eating in adult female twins (N = 571) drawn from the MSU Twin Registry. Emotional eating, estradiol levels, and progesterone levels were assessed daily and then averaged to create aggregate measures for analysis. As predicted, shared environmental influences were significantly greater in twins with high estradiol levels, whereas additive genetic effects increased substantially across low versus high progesterone groups. Results highlight significant and differential effects of ovarian hormones on etiologic risk for emotional eating in adulthood.
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Date Issued: 2016-09-01
Identifier: FSU_pmch_27747142, 10.1177/2167702616641637, PMC5063244, 27747142, 27747142
Format: Citation

Title: A Critical Evaluation of the Down Syndrome Diagnosis for LB1, Type Specimen of Homo floresiensis.
Creator: Baab, Karen L., Brown, Peter, Falk, Dean, Richtsmeier, Joan T., Hildebolt, Charles F., Smith, Kirk, Jungers, William
Abstract/Description: The Liang Bua hominins from Flores, Indonesia, have been the subject of intense scrutiny and debate since their initial description and classification in 2004. These remains have been assigned to a new species, Homo floresiensis, with the partial skeleton LB1 as the type specimen. The Liang Bua hominins are notable for their short stature, small endocranial volume, and many features that appear phylogenetically primitive relative to modern humans, despite their late Pleistocene age. Recently,...
Show moreThe Liang Bua hominins from Flores, Indonesia, have been the subject of intense scrutiny and debate since their initial description and classification in 2004. These remains have been assigned to a new species, Homo floresiensis, with the partial skeleton LB1 as the type specimen. The Liang Bua hominins are notable for their short stature, small endocranial volume, and many features that appear phylogenetically primitive relative to modern humans, despite their late Pleistocene age. Recently, some workers suggested that the remains represent members of a small-bodied island population of modern Austro-Melanesian humans, with LB1 exhibiting clinical signs of Down syndrome. Many classic Down syndrome signs are soft tissue features that could not be assessed in skeletal remains. Moreover, a definitive diagnosis of Down syndrome can only be made by genetic analysis as the phenotypes associated with Down syndrome are variable. Most features that contribute to the Down syndrome phenotype are not restricted to Down syndrome but are seen in other chromosomal disorders and in the general population. Nevertheless, we re-evaluated the presence of those phenotypic features used to support this classification by comparing LB1 to samples of modern humans diagnosed with Down syndrome and euploid modern humans using comparative morphometric analyses. We present new data regarding neurocranial, brain, and symphyseal shape in Down syndrome, additional estimates of stature for LB1, and analyses of inter- and intralimb proportions. The presence of cranial sinuses is addressed using CT images of LB1. We found minimal congruence between the LB1 phenotype and clinical descriptions of Down syndrome. We present important differences between the phenotypes of LB1 and individuals with Down syndrome, and quantitative data that characterize LB1 as an outlier compared with Down syndrome and non-Down syndrome groups. Homo floresiensis remains a phenotypically unique, valid species with its roots in Plio-Pleistocene Homo taxa.
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Date Issued: 2016-06-08
Identifier: FSU_libsubv1_wos_000377561700008, 10.1371/journal.pone.0155731
Format: Citation

Title: A Landmark-Free Method for Three-Dimensional Shape Analysis.
Creator: Pomidor, Benjamin J., Makedonska, Jana, Slice, Dennis E.
Abstract/Description: Background The tools and techniques used in morphometrics have always aimed to transform the physical shape of an object into a concise set of numerical data for mathematical analysis. The advent of landmark-based morphometrics opened new avenues of research, but these methods are not without drawbacks. The time investment required of trained individuals to accurately landmark a data set is significant, and the reliance on readily-identifiable physical features can hamper research efforts....
Show moreBackground The tools and techniques used in morphometrics have always aimed to transform the physical shape of an object into a concise set of numerical data for mathematical analysis. The advent of landmark-based morphometrics opened new avenues of research, but these methods are not without drawbacks. The time investment required of trained individuals to accurately landmark a data set is significant, and the reliance on readily-identifiable physical features can hamper research efforts. This is especially true of those investigating smooth or featureless surfaces. Methods In this paper, we present a new method to perform this transformation for data obtained from high-resolution scanning technology. This method uses surface scans, instead of landmarks, to calculate a shape difference metric analogous to Procrustes distance and perform superimposition. This is accomplished by building upon and extending the Iterative Closest Point algorithm. We also explore some new ways this data can be used; for example, we can calculate an averaged surface directly and visualize point-wise shape information over this surface. Finally, we briefly demonstrate this method on a set of primate skulls and compare the results of the new methodology with traditional geometric morphometric analysis.
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Date Issued: 2016-03-08
Identifier: FSU_libsubv1_wos_000371991300024, 10.1371/journal.pone.0150368
Format: Citation

Title: A Comparison of Rule-based Analysis with Regression Methods in Understanding the Risk Factors for Study Withdrawal in a Pediatric Study.
Creator: Haghighi, Mona, Johnson, Suzanne Bennett, Qian, Xiaoning, Lynch, Kristian F., Vehik, Kendra, Huang, Shuai
Abstract/Description: Regression models are extensively used in many epidemiological studies to understand the linkage between specific outcomes of interest and their risk factors. However, regression models in general examine the average effects of the risk factors and ignore subgroups with different risk profiles. As a result, interventions are often geared towards the average member of the population, without consideration of the special health needs of different subgroups within the population. This paper...
Show moreRegression models are extensively used in many epidemiological studies to understand the linkage between specific outcomes of interest and their risk factors. However, regression models in general examine the average effects of the risk factors and ignore subgroups with different risk profiles. As a result, interventions are often geared towards the average member of the population, without consideration of the special health needs of different subgroups within the population. This paper demonstrates the value of using rule-based analysis methods that can identify subgroups with heterogeneous risk profiles in a population without imposing assumptions on the subgroups or method. The rules define the risk pattern of subsets of individuals by not only considering the interactions between the risk factors but also their ranges. We compared the rule-based analysis results with the results from a logistic regression model in The Environmental Determinants of Diabetes in the Young (TEDDY) study. Both methods detected a similar suite of risk factors, but the rule-based analysis was superior at detecting multiple interactions between the risk factors that characterize the subgroups. A further investigation of the particular characteristics of each subgroup may detect the special health needs of the subgroup and lead to tailored interventions.
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Date Issued: 2016-08-26
Identifier: FSU_libsubv1_wos_000381966300001, 10.1038/srep30828
Format: Citation

Title: 14-3-3τ promotes surface expression of Cav2.2 (α1B) Ca2+ channels.
Creator: Liu, Feng, Zhou, Qin, Zhou, Jie, Sun, Hao, Wang, Yan, Zou, Xiuqun, Feng, Lingling, Hou, Zhaoyuan, Zhou, Aiwu, Zhou, Yi, Li, Yong
Abstract/Description: Surface expression of voltage-gated Ca(2+) (Cav) channels is important for their function in calcium homeostasis in the physiology of excitable cells, but whether or not and how the α1 pore-forming subunits of Cav channels are trafficked to plasma membrane in the absence of the known Cav auxiliary subunits, β and α2δ, remains mysterious. Here we showed that 14-3-3 proteins promoted functional surface expression of the Cav2.2 α1B channel in transfected tsA-201 cells in the absence of any known...
Show moreSurface expression of voltage-gated Ca(2+) (Cav) channels is important for their function in calcium homeostasis in the physiology of excitable cells, but whether or not and how the α1 pore-forming subunits of Cav channels are trafficked to plasma membrane in the absence of the known Cav auxiliary subunits, β and α2δ, remains mysterious. Here we showed that 14-3-3 proteins promoted functional surface expression of the Cav2.2 α1B channel in transfected tsA-201 cells in the absence of any known Cav auxiliary subunit. Both the surface to total ratio of the expressed α1B protein and the current density of voltage step-evoked Ba(2+) current were markedly suppressed by the coexpression of a 14-3-3 antagonist construct, pSCM138, but not its inactive control, pSCM174, as determined by immunofluorescence assay and whole cell voltage clamp recording, respectively. By contrast, coexpression with 14-3-3τ significantly enhanced the surface expression and current density of the Cav2.2 α1B channel. Importantly, we found that between the two previously identified 14-3-3 binding regions at the α1B C terminus, only the proximal region (amino acids 1706-1940), closer to the end of the last transmembrane domain, was retained by the endoplasmic reticulum and facilitated by 14-3-3 to traffic to plasma membrane. Additionally, we showed that the 14-3-3/Cav β subunit coregulated the surface expression of Cav2.2 channels in transfected tsA-201 cells and neurons. Altogether, our findings reveal a previously unidentified regulatory function of 14-3-3 proteins in promoting the surface expression of Cav2.2 α1B channels.
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Date Issued: 2015-01-30
Identifier: FSU_pmch_25516596, 10.1074/jbc.M114.567800, PMC4317001, 25516596, 25516596, M114.567800
Format: Citation

Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes.
Creator: Xu, Zhe, Graham, Kourtney, Foote, Molly, Liang, Fengshan, Rizkallah, Raed, Hurt, Myra, Wang, Yanchang, Wu, Yuying, Zhou, Yi
Abstract/Description: The aggresome is a key cytoplasmic organelle for sequestration and clearance of toxic protein aggregates. Although loading misfolded proteins cargos to dynein motors has been recognized as an important step in the aggresome formation process, the molecular machinery that mediates the association of cargos with the dynein motor is poorly understood. Here, we report a new aggresome-targeting pathway that involves isoforms of 14-3-3, a family of conserved regulatory proteins. 14-3-3 interacts...
Show moreThe aggresome is a key cytoplasmic organelle for sequestration and clearance of toxic protein aggregates. Although loading misfolded proteins cargos to dynein motors has been recognized as an important step in the aggresome formation process, the molecular machinery that mediates the association of cargos with the dynein motor is poorly understood. Here, we report a new aggresome-targeting pathway that involves isoforms of 14-3-3, a family of conserved regulatory proteins. 14-3-3 interacts with both the dynein-intermediate chain (DIC) and an Hsp70 co-chaperone Bcl-2-associated athanogene 3 (BAG3), thereby recruiting chaperone-associated protein cargos to dynein motors for their transport to aggresomes. This molecular cascade entails functional dimerization of 14-3-3, which we show to be crucial for the formation of aggresomes in both yeast and mammalian cells. These results suggest that 14-3-3 functions as a molecular adaptor to promote aggresomal targeting of misfolded protein aggregates and may link such complexes to inclusion bodies observed in various neurodegenerative diseases.
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Date Issued: 2013-09-15
Identifier: FSU_pmch_23843611, 10.1242/jcs.126102, PMC3772389, 23843611, 23843611, jcs.126102
Format: Citation

Title: 14-3-3 proteins in neurological disorders.
Creator: Foote, Molly, Zhou, Yi
Abstract/Description: 14-3-3 proteins were originally discovered as a family of proteins that are highly expressed in the brain. Through interactions with a multitude of binding partners, 14-3-3 proteins impact many aspects of brain function including neural signaling, neuronal development and neuroprotection. Although much remains to be learned and understood, 14-3-3 proteins have been implicated in a variety of neurological disorders based on evidence from both clinical and laboratory studies. Here we will...
Show more14-3-3 proteins were originally discovered as a family of proteins that are highly expressed in the brain. Through interactions with a multitude of binding partners, 14-3-3 proteins impact many aspects of brain function including neural signaling, neuronal development and neuroprotection. Although much remains to be learned and understood, 14-3-3 proteins have been implicated in a variety of neurological disorders based on evidence from both clinical and laboratory studies. Here we will review previous and more recent research that has helped us understand the roles of 14-3-3 proteins in both neurodegenerative and neuropsychiatric diseases.
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Date Issued: 2012-01-01
Identifier: FSU_pmch_22773956, PMC3388734, 22773956, 22773956
Format: Citation

Title: 14-3-3 proteins are required for hippocampal long-term potentiation and associative learning and memory.
Creator: Qiao, Haifa, Foote, Molly, Graham, Kourtney, Wu, Yuying, Zhou, Yi
Abstract/Description: 14-3-3 is a family of regulatory proteins highly expressed in the brain. Previous invertebrate studies have demonstrated the importance of 14-3-3 in the regulation of synaptic functions and learning and memory. However, the in vivo role of 14-3-3 in these processes has not been determined using mammalian animal models. Here, we report the behavioral and electrophysiological characterization of a new animal model of 14-3-3 proteins. These transgenic mice, considered to be a 14-3-3 functional...
Show more14-3-3 is a family of regulatory proteins highly expressed in the brain. Previous invertebrate studies have demonstrated the importance of 14-3-3 in the regulation of synaptic functions and learning and memory. However, the in vivo role of 14-3-3 in these processes has not been determined using mammalian animal models. Here, we report the behavioral and electrophysiological characterization of a new animal model of 14-3-3 proteins. These transgenic mice, considered to be a 14-3-3 functional knock-out, express a known 14-3-3 inhibitor in various brain regions of different founder lines. We identify a founder-specific impairment in hippocampal-dependent learning and memory tasks, as well as a correlated suppression in long-term synaptic plasticity of the hippocampal synapses. Moreover, hippocampal synaptic NMDA receptor levels are selectively reduced in the transgenic founder line that exhibits both behavioral and synaptic plasticity deficits. Collectively, our findings provide evidence that 14-3-3 is a positive regulator of associative learning and memory at both the behavioral and cellular level.
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Date Issued: 2014-04-02
Identifier: FSU_pmch_24695700, 10.1523/JNEUROSCI.4393-13.2014, PMC3972712, 24695700, 24695700, 34/14/4801
Format: Citation

Title: The 2.8 Angstrom Electron Microscopy Structure Of Adeno-associated Virus-dj Bound By A Heparinoid Pentasaccharide.
Creator: Xie, Qing, Spear, John M., Noble, Alex J., Sousa, Duncan R., Meyer, Nancy L., Davulcu, Omar, Zhang, Fuming, Linhardt, Robert J., Stagg, Scott M., Chapman, Michael S.
Abstract/Description: Atomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with fondaparinux, have been determined by cryoelectron microscopy at 3 angstrom resolution. The gene therapy vector, AAV-DJ, is a hybrid of natural serotypes that was previously derived by directed evolution, selecting for hepatocyte entry and resistance to neutralization by human serum. The structure of AAV-DJ differs from that of parental serotypes in two regions where neutralizing antibodies bind, so immune escape...
Show moreAtomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with fondaparinux, have been determined by cryoelectron microscopy at 3 angstrom resolution. The gene therapy vector, AAV-DJ, is a hybrid of natural serotypes that was previously derived by directed evolution, selecting for hepatocyte entry and resistance to neutralization by human serum. The structure of AAV-DJ differs from that of parental serotypes in two regions where neutralizing antibodies bind, so immune escape appears to have been the primary driver of AAV-DJ's directed evolution. Fondaparinux is an analog of cell surface heparan sulfate to which several AAVs bind during entry. Fondaparinux interacts with viral arginines at a known heparin binding site, without the large conformational changes whose presence was controversial in low-resolution imaging of AAV2-heparin complexes. The glycan density suggests multimodal binding that could accommodate sequence variation and multivalent binding along a glycan polymer, consistent with a role in attachment, prior to more specific interactions with a receptor protein mediating entry.
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Date Issued: 2017-06
Identifier: FSU_libsubv1_wos_000406299600001, 10.1016/j.omtm.2017.02.004
Format: Citation

Title: 14-3-3 and aggresome formation: implications in neurodegenerative diseases..
Creator: Jia, Baohui, Wu, Yuying, Zhou, Yi
Abstract/Description: Protein misfolding and aggregation underlie the pathogenesis of many neurodegenerative diseases. In addition to chaperone-mediated refolding and proteasomal degradation, the aggresome-macroautophagy pathway has emerged as another defense mechanism for sequestration and clearance of toxic protein aggregates in cells. Previously, the 14-3-3 proteins were shown to be indispensable for the formation of aggresomes induced by mutant huntingtin proteins. In a recent study, we have determined that 14...
Show moreProtein misfolding and aggregation underlie the pathogenesis of many neurodegenerative diseases. In addition to chaperone-mediated refolding and proteasomal degradation, the aggresome-macroautophagy pathway has emerged as another defense mechanism for sequestration and clearance of toxic protein aggregates in cells. Previously, the 14-3-3 proteins were shown to be indispensable for the formation of aggresomes induced by mutant huntingtin proteins. In a recent study, we have determined that 14-3-3 functions as a molecular adaptor to recruit chaperone-associated misfolded proteins to dynein motors for transport to aggresomes. This molecular complex involves a dimeric binding of 14-3-3 to both the dynein-intermediate chain (DIC) and an Hsp70 co-chaperone Bcl-2-associated athanogene 3 (BAG3). As 14-3-3 has been implicated in various neurodegenerative diseases, our findings may provide mechanistic insights into its role in managing misfolded protein stress during the process of neurodegeneration.
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Date Issued: 2014-03-01
Identifier: FSU_pmch_24549097, PMC4189886, 24549097, 24549097, 28123
Format: Citation

Title: 24/7 Access: Crafted in 2010 and Built to Last.
Creator: Rosasco, Robyn, Epstein, Susan A.
Abstract/Description: Objectives Our library has provided 24/7 access for student study space since 2010. This service has functioned as anticipated with minor policy breaches in 2010 and 2013. The purpose of this study was to determine how well 24/7 access to our library is working, obtain student feedback on security procedures, and solicit ideas from all stakeholders for future improvements. Methods Stakeholders for 24/7 access to our academic health sciences library include both the service providers (library,...
Show moreObjectives Our library has provided 24/7 access for student study space since 2010. This service has functioned as anticipated with minor policy breaches in 2010 and 2013. The purpose of this study was to determine how well 24/7 access to our library is working, obtain student feedback on security procedures, and solicit ideas from all stakeholders for future improvements. Methods Stakeholders for 24/7 access to our academic health sciences library include both the service providers (library, student affairs, and security/legal personnel) and the service users (first- and second-year medical students and biomedical graduate students). To evaluate the effectiveness of the library's 24/7 access service, the authors conducted a series of free-response interviews with several service provider and user stakeholders to solicit the most detailed feedback possible. The authors also administered a brief survey to determine if students were satisfied with unstaffed access to library study space. The results of stakeholder feedback were used to evaluate the value of our extensive planning for 24/7 access to the library, our thorough and consistent implementation, and our responsiveness to the needs of our students. Results: Our library director invited (via email) 14 student/staff stakeholders to be interviewed by the primary investigator (PI) and co-PI. Three students and three staff members agreed to be interviewed; five interviews were conducted face-to-face and one was conducted by phone. In addition, our library director sent an email invitation to complete a 10-question survey to approximately 500 students; 114 surveys were completed. From the interviews and the survey, shared themes as well as several unique feedback items emerged. In general, the overwhelming response was that the 24/7 service meets the needs of students, providing a secure, consistent study area for students who do not choose to study in another College of Medicine study space, in another campus library, at home, or at another location. Specifically, 90% of the students were not concerned about safety/security issues in the library during unstaffed hours, 82% indicated that the library environment was conducive to studying during unstaffed hours, and 83% felt that the library's orientation on 24/7 policies and procedures has met their needs. Conclusions: Collecting formal feedback on our 24/7 service has validated the time spent in planning, implementation, and responding to student needs, and has also elicited several ideas for improving the 24/7 service. We plan to continue interviewing and surveying 24/7 stakeholders on a yearly basis to solicit current feedback and continually improve the service.
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Date Issued: 2014
Identifier: FSU_migr_medlib_posters-0003
Format: Citation

Title: 4th International Digital Curation Conference - Minute Madness: Poster Session (slide # 8).
Creator: Smith, Plato
Abstract/Description: This poster session will use text, diagrams, and images to display the development of the application of The DCC Curation Lifecycle Model practices to preservation of Diatomscapes. Diatomscapes represents a collection of images of biological silica and includes diatoms ("microscopic, single-celled plants that thrive in freshwater, saltwater, brackish water and even semi-terrestrial environments" (Prasad, 2005)) and Radiolarians ("any of various marine protozoans of the order Radiolaria,...
Show moreThis poster session will use text, diagrams, and images to display the development of the application of The DCC Curation Lifecycle Model practices to preservation of Diatomscapes. Diatomscapes represents a collection of images of biological silica and includes diatoms ("microscopic, single-celled plants that thrive in freshwater, saltwater, brackish water and even semi-terrestrial environments" (Prasad, 2005)) and Radiolarians ("any of various marine protozoans of the order Radiolaria, having rigid siliceous skeletons and spicules" (Dictionary, 2008)).
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Date Issued: 2008
Identifier: FSU_migr_digital_lib-0002
Format: Citation

Title: After DSM-5: A Critical Mental Health Research Agenda for the 21st Century.
Creator: Lacasse, Jeffrey R.
Date Issued: 2014
Identifier: FSU_migr_csw_faculty_publications-0072, 10.1177/1049731513510048
Format: Citation

Title: Advancing Behavioural Genomics By Considering Timescale.
Creator: Rittschof, Clare C., Hughes, Kimberly A.
Abstract/Description: Animal behavioural traits often covary with gene expression, pointing towards a genomic constraint on organismal responses to environmental cues. This pattern highlights a gap in our understanding of the time course of environmentally responsive gene expression, and moreover, how these dynamics are regulated. Advances in behavioural genomics explore how gene expression dynamics are correlated with behavioural traits that range from stable to highly labile. We consider the idea that certain...
Show moreAnimal behavioural traits often covary with gene expression, pointing towards a genomic constraint on organismal responses to environmental cues. This pattern highlights a gap in our understanding of the time course of environmentally responsive gene expression, and moreover, how these dynamics are regulated. Advances in behavioural genomics explore how gene expression dynamics are correlated with behavioural traits that range from stable to highly labile. We consider the idea that certain genomic regulatory mechanisms may predict the timescale of an environmental effect on behaviour. This temporally minded approach could inform both organismal and evolutionary questions ranging from the remediation of early life social trauma to understanding the evolution of trait plasticity.
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Date Issued: 2018-02-12
Identifier: FSU_libsubv1_wos_000424747100001, 10.1038/s41467-018-02971-0
Format: Citation

Title: Advanced Reading Comprehension Expectations in Secondary School: Considerations for Students with Emotional or Behavior Disorders..
Creator: Ciullo, Stephen, Ortiz, Miriam B, Al Otaiba, Stephanie, Lane, Kathleen Lynne
Abstract/Description: The debate around recent implementation of the Common Core Standards (CCSS) has perplexed many policy makers, practitioners, and researchers; yet there remains broad agreement for the need to improve reading outcomes and college and career readiness for all students, including students with disabilities. One of the most vulnerable populations with disabilities in terms of college and career readiness is students with emotional disorders (ED). A considerable percentage of students with ED...
Show moreThe debate around recent implementation of the Common Core Standards (CCSS) has perplexed many policy makers, practitioners, and researchers; yet there remains broad agreement for the need to improve reading outcomes and college and career readiness for all students, including students with disabilities. One of the most vulnerable populations with disabilities in terms of college and career readiness is students with emotional disorders (ED). A considerable percentage of students with ED encounter unfavorable academic and long-term outcomes, often due to reading difficulties and behavioral variables that impede learning. To date, the impact of rising expectations in reading on the education of students with ED has been absent from this conversation about CCSS. In this article, we consider the implications of new reading expectations in the critical period of Grades 6-12 for students with ED. First, we summarize grade level expectations of the standards. Then, we describe the characteristics and underachievement of students with ED. Next, we evaluate challenges in meeting the expectations based on extant research, and provide recommendations for practice based on the intervention literature. We conclude by prioritizing a research and policy agenda that advocates for increasing the likelihood of success in reading for students with ED in middle school and high school.
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Date Issued: 2016-06-01
Identifier: FSU_pmch_27403040, 10.1177/1044207315604365, PMC4937879, 27403040, 27403040
Format: Citation

Title: Aerobic Exercise and Whole-Body Vibration in Offsetting Bone Loss in Older Adults.
Creator: Liu, Pei-Yang, Brummel-Smith, Kenneth, Ilich, Jasminka
Abstract/Description: Osteoporosis and its associated fractures are common complications of aging and most strategies to prevent and/or treat bone loss focused on antiresorptive medications. However, aerobic exercise (AEX) and/or whole-body vibration (WBV) might have beneficial effect on bone mass and provide an alternative approach to increase or maintain bone mineral density (BMD) and reduce the risk of fractures. The purpose of this paper was to investigate the potential benefits of AEX and WBV on BMD in older...
Show moreOsteoporosis and its associated fractures are common complications of aging and most strategies to prevent and/or treat bone loss focused on antiresorptive medications. However, aerobic exercise (AEX) and/or whole-body vibration (WBV) might have beneficial effect on bone mass and provide an alternative approach to increase or maintain bone mineral density (BMD) and reduce the risk of fractures. The purpose of this paper was to investigate the potential benefits of AEX and WBV on BMD in older population and discuss the possible mechanisms of action. Several online databases were utilized and based on the available literature the consensus is that both AEX and WBV may increase spine and femoral BMD in older adults. Therefore, AEX and WBV could serve as nonpharmacological and complementary approaches to increasing/maintaining BMD. However, it is uncertain if noted effects could be permanent and further studies are needed to investigate sustainability of either type of the exercise.
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Date Issued: 2011
Identifier: FSU_migr_geriatrics_faculty_publications-0042, 10.4061/2011/379674
Format: Citation

Title: Advancing Conservation By Understanding And Influencing Human Behavior.
Creator: Reddy, Sheila M. W., Montambault, Jensen, Masuda, Yuta J., Keenan, Elizabeth, Butler, William, Fisher, Jonathan R. B., Asah, Stanley T., Gneezy, Ayelet
Abstract/Description: Behavioral sciences can advance conservation by systematically identifying behavioral barriers to conservation and how to best overcome them. Behavioral sciences have informed policy in many other realms (e.g., health, savings), but they are a largely untapped resource for conservation. We propose a set of guiding questions for applying behavioral insights to conservation policy. These questions help define the conservation problem as a behavior change problem, understand behavioral...
Show moreBehavioral sciences can advance conservation by systematically identifying behavioral barriers to conservation and how to best overcome them. Behavioral sciences have informed policy in many other realms (e.g., health, savings), but they are a largely untapped resource for conservation. We propose a set of guiding questions for applying behavioral insights to conservation policy. These questions help define the conservation problem as a behavior change problem, understand behavioral mechanisms and identify appropriate approaches for behavior change (awareness, incentives, nudges), and evaluate and adapt approaches based on new behavioral insights. We provide a foundation for the questions by synthesizing a wide range of behavior change models and evidence related to littering, water and energy conservation, and land management. We also discuss the methodology and data needed to answer these questions. We illustrate how these questions have been answered in practice to inform efforts to promote conservation for climate risk reduction. Although more comprehensive research programs to answer these questions are needed, some insights are emerging. Integrating two or more behavior change approaches that target multiple, context-dependent factors may be most successful; however, caution must be taken to avoid approaches that could undermine one another (e.g., economic incentives crowding out intrinsic incentives).
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Date Issued: 2017-04
Identifier: FSU_libsubv1_wos_000398563500010, 10.1111/conl.12252
Format: Citation

Title: Advanced (Measurement) Applications of Curriculum-Based Measurement in Reading.
Creator: Petscher, Yaacov, Cummings, Kelli Dawn, Biancarosa, Gina, Fien, Hank
Abstract/Description: The purpose of this article is to provide a commentary on the current state of several measurement issues pertaining to curriculum-based measures of reading (R-CBM). We begin by providing an overview of the utility of R-CBM, followed by a presentation of five specific measurements considerations: 1) the reliability of R-CBM oral reading fluency, 2) issues pertaining to form effects, 3) the generalizability of scores from R-CBM, 4) measurement error, and 5) linearity of growth in R-CBM. We...
Show moreThe purpose of this article is to provide a commentary on the current state of several measurement issues pertaining to curriculum-based measures of reading (R-CBM). We begin by providing an overview of the utility of R-CBM, followed by a presentation of five specific measurements considerations: 1) the reliability of R-CBM oral reading fluency, 2) issues pertaining to form effects, 3) the generalizability of scores from R-CBM, 4) measurement error, and 5) linearity of growth in R-CBM. We then conclude with a presentation of the purpose for this issue and broadly introduce the articles in the special issue. Because oral reading fluency is one of the most common measures of R-CBM, much of the review is focused on this particular type of assessment; however, the issues presented extend to other assessments of R-CBM.
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Date Issued: 2013-03-01
Identifier: FSU_pmch_26346551, 10.1177/1534508412461434, PMC4557774, 26346551, 26346551
Format: Citation

Title: Advanced (Measurement) Applications of Curriculum-based Measurement in Reading.
Creator: Petscher, Yaacov M., Cummings, Kelli, Biancarosa, Gina, Fien, Hank
Abstract/Description: The purpose of this article is to provide a commentary on the current state of several measurement issues pertaining to curriculum-based measures of reading (R-CBM1). We begin by providing an overview of the utility of R-CBM, followed by a presentation of five specific measurements considerations: 1) the reliability of R-CBM oral reading fluency, 2) issues pertaining to form effects, 3) the generalizability of scores from R-CBM, 4) measurement error, and 5) linearity of growth in R-CBM. We...
Show moreThe purpose of this article is to provide a commentary on the current state of several measurement issues pertaining to curriculum-based measures of reading (R-CBM1). We begin by providing an overview of the utility of R-CBM, followed by a presentation of five specific measurements considerations: 1) the reliability of R-CBM oral reading fluency, 2) issues pertaining to form effects, 3) the generalizability of scores from R-CBM, 4) measurement error, and 5) linearity of growth in R-CBM. We then conclude with a presentation of the purpose for this issue and broadly introduce the articles in the special issue. Because oral reading fluency is one of the most common measures of R-CBM, much of the review is focused on this particular type of assessment; however, the issues presented extend to other assessments of R-CBM.
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Date Issued: 2013
Identifier: FSU_migr_fcrr-pubs-0001, 10.1177/1534508412461434
Format: Citation

Title: An Assessment of Multimodel Simulations for the Variability of Western North Pacific Tropical Cyclones and Its Association with ENSO.
Creator: Han, Rongqing, Wang, Hui, Hu, Zeng-Zhen, Kumar, Arun, Li, Weijing, Long, Lindsey N., Schemm, Jae-Kyung E., Peng, Peitao, Wang, Wanqiu, Si, Dong, Jia, Xiaolong, Zhao, Ming,...
Show moreHan, Rongqing, Wang, Hui, Hu, Zeng-Zhen, Kumar, Arun, Li, Weijing, Long, Lindsey N., Schemm, Jae-Kyung E., Peng, Peitao, Wang, Wanqiu, Si, Dong, Jia, Xiaolong, Zhao, Ming, Vecchi, Gabriel A., Larow, Timothy E., Lim, Young-Kwon, Schubert, Siegfried D., Camargo, Suzana J., Henderson, Naomi, Jonas, Jeffrey A., Walsh, Kevin J. E.
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Abstract/Description: An assessment of simulations of the interannual variability of tropical cyclones (TCs) over the western North Pacific (WNP) and its association with El Nino-Southern Oscillation (ENSO), as well as a subsequent diagnosis for possible causes of model biases generated from simulated large-scale climate conditions, are documented in the paper. The model experiments are carried out by the Hurricane Work Group under the U.S. Climate Variability and Predictability Research Program (CLIVAR) using...
Show moreAn assessment of simulations of the interannual variability of tropical cyclones (TCs) over the western North Pacific (WNP) and its association with El Nino-Southern Oscillation (ENSO), as well as a subsequent diagnosis for possible causes of model biases generated from simulated large-scale climate conditions, are documented in the paper. The model experiments are carried out by the Hurricane Work Group under the U.S. Climate Variability and Predictability Research Program (CLIVAR) using five global climate models (GCMs) with a total of 16 ensemble members forced by the observed sea surface temperature and spanning the 28-yr period from 1982 to 2009. The results show GISS and GFDL model ensemble means best simulate the interannual variability of TCs, and the multimodel ensemble mean (MME) follows. Also, the MME has the closest climate mean annual number of WNP TCs and the smallest root-mean-square error to the observation. Most GCMs can simulate the interannual variability of WNP TCs well, with stronger TC activities during two types of El Nino-namely, eastern Pacific (EP) and central Pacific (CP) El Nino-and weaker activity during La Nina. However, none of the models capture the differences in TC activity between EP and CP El Nino as are shown in observations. The inability of models to distinguish the differences in TC activities between the two types of El Nino events may be due to the bias of the models in response to the shift of tropical heating associated with CP El Nino.
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Date Issued: 2016-09-15
Identifier: FSU_libsubv1_wos_000383828300001, 10.1175/JCLI-D-15-0720.1
Format: Citation

Title: Alteration Of Neuronal Excitability And Short-term Synaptic Plasticity In The Prefrontal Cortex Of A Mouse Model Of Mental Illness.
Creator: Crabtree, Gregg W., Sun, Ziyi, Kvajo, Mirna, Broek, Jantine A. C., Fenelon, Karine, McKellar, Heather, Xiao, Lan, Xu, Bin, Bahn, Sabine, O'Donnell, James M., Gogos, Joseph A.
Abstract/Description: Using a genetic mouse model that faithfully recapitulates a DISC1 genetic alteration strongly associated with schizophrenia and other psychiatric disorders, we examined the impact of this mutation within the prefrontal cortex. Although cortical layering, cytoarchitecture, and proteome were found to be largely unaffected, electrophysiological examination of the mPFC revealed both neuronal hyperexcitability and alterations in short-term synaptic plasticity consistent with enhanced...
Show moreUsing a genetic mouse model that faithfully recapitulates a DISC1 genetic alteration strongly associated with schizophrenia and other psychiatric disorders, we examined the impact of this mutation within the prefrontal cortex. Although cortical layering, cytoarchitecture, and proteome were found to be largely unaffected, electrophysiological examination of the mPFC revealed both neuronal hyperexcitability and alterations in short-term synaptic plasticity consistent with enhanced neurotransmitter release. Increased excitability of layer II/III pyramidal neurons was accompanied by consistent reductions in voltage-activated potassium currents near the action potential threshold as well as by enhanced recruitment of inputs arising from superficial layers to layer V. We further observed reductions in both the paired-pulse ratios and the enhanced short-term depression of layer V synapses arising from superficial layers consistent with enhanced neurotransmitter release at these synapses. Recordings from layer II/III pyramidal neurons revealed action potential widening that could account for enhanced neurotransmitter release. Significantly, we found that reduced functional expression of the voltagedependent potassium channel subunit K(v)1.1 substantially contributes to both the excitability and short-term plasticity alterations that we observed. The underlying dysregulation of K(v)1.1 expression was attributable to cAMP elevations in the PFC secondary to reduced phosphodiesterase 4 activity present in Disc1 deficiency and was rescued by pharmacological blockade of adenylate cyclase. Our results demonstrate a potentially devastating impact of Disc1 deficiency on neural circuit function, partly due to Kv1.1 dysregulation that leads to a dual dysfunction consisting of enhanced neuronal excitability and altered short-term synaptic plasticity.
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Date Issued: 2017-04-12
Identifier: FSU_libsubv1_wos_000399440400015, 10.1523/JNEUROSCI.4345-15.2017
Format: Citation

Title: Amide Hydrogens Reveal A Temperature-dependent Structural Transition That Enhances Site-ii Ca2+ -binding Affinity In A C-domain Mutant Of Cardiac Troponin C.
Creator: Veltri, Tiago, de Oliveira, Guilherme A. P., Bienkiewicz, Ewa A., Palhano, Fernando L., Marques, Mayra de A., Moraes, Adolfo H., Silva, Jerson L., Sorenson, Martha M., Pinto,...
Show moreVeltri, Tiago, de Oliveira, Guilherme A. P., Bienkiewicz, Ewa A., Palhano, Fernando L., Marques, Mayra de A., Moraes, Adolfo H., Silva, Jerson L., Sorenson, Martha M., Pinto, Jose R.
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Abstract/Description: The hypertrophic cardiomyopathy-associated mutant D145E, in cardiac troponin C (cTnC) C-domain, causes generalised instability at multiple sites in the isolated protein. As a result, structure and function of the mutant are more susceptible to higher temperatures. Above 25 degrees C there are large, progressive increases in N-domain Ca2+-binding affinity for D145E but only small changes for the wild-type protein. NMR-derived backbone amide temperature coefficients for many residues show a...
Show moreThe hypertrophic cardiomyopathy-associated mutant D145E, in cardiac troponin C (cTnC) C-domain, causes generalised instability at multiple sites in the isolated protein. As a result, structure and function of the mutant are more susceptible to higher temperatures. Above 25 degrees C there are large, progressive increases in N-domain Ca2+-binding affinity for D145E but only small changes for the wild-type protein. NMR-derived backbone amide temperature coefficients for many residues show a sharp transition above 30-40 degrees C, indicating a temperature-dependent conformational change that is most prominent around the mutated EF-hand IV, as well as throughout the C-domain. Smaller, isolated changes occur in the N-domain. Cardiac skinned fibres reconstituted with D145E are more sensitive to Ca2+ than fibres reconstituted with wild-type, and this defect is amplified near body-temperature. We speculate that the D145E mutation destabilises the native conformation of EF-hand IV, leading to a transient unfolding and dissociation of helix H that becomes more prominent at higher temperatures. This creates exposed hydrophobic surfaces that may be capable of binding unnaturally to a variety of targets, possibly including the N-domain of cTnC when it is in its open Ca2+-saturated state. This would constitute a potential route for propagating signals from one end of TnC to the other.
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Date Issued: 2017-04-06
Identifier: FSU_libsubv1_wos_000398545900010, 10.1038/s41598-017-00777-6
Format: Citation

Title: An Alternative to the Search for Single Polymorphisms: Toward Molecular Personality Scales for the Five-Factor Model.
Creator: McCrae, Robert R., Scally, Matthew, Terracciano, Antonio, Abecasis, Gonçalo, Costa, Paul
Abstract/Description: There is growing evidence that personality traits are affected by many genes, all of which have very small effects. As an alternative to the largely unsuccessful search for individual polymorphisms associated with personality traits, the authors identified large sets of potentially related single nucleotide polymorphisms (SNPs) and summed them to form molecular personality scales (MPSs) with from 4 to 2,497 SNPs. Scales were derived from two thirds of a large (N = 3,972) sample of individuals...
Show moreThere is growing evidence that personality traits are affected by many genes, all of which have very small effects. As an alternative to the largely unsuccessful search for individual polymorphisms associated with personality traits, the authors identified large sets of potentially related single nucleotide polymorphisms (SNPs) and summed them to form molecular personality scales (MPSs) with from 4 to 2,497 SNPs. Scales were derived from two thirds of a large (N = 3,972) sample of individuals from Sardinia who completed the Revised NEO Personality Inventory (P. T. Costa, Jr., & R. R. McCrae, 1992) and were assessed in a genomewide association scan. When MPSs were correlated with the phenotype in the remaining one third of the sample, very small but significant associations were found for 4 of the 5e personality factors when the longest scales were examined. These data suggest that MPSs for Neuroticism, Openness to Experience, Agreeableness, and Conscientiousness (but not Extraversion) contain genetic information that can be refined in future studies, and the procedures described here should be applicable to other quantitative traits.
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Date Issued: 2010
Identifier: FSU_migr_geriatrics_faculty_publications-0015, 10.1037/a0020964
Format: Citation

Title: Amide hydrogens reveal a temperature-dependent structural transition that enhances site-II Ca(2+)-binding affinity in a C-domain mutant of cardiac troponin C.
Creator: Veltri, Tiago, de Oliveira, Guilherme A P, Bienkiewicz, Ewa A, Palhano, Fernando L, Marques, Mayra de A, Moraes, Adolfo H, Silva, Jerson L, Sorenson, Martha M, Pinto, Jose R
Abstract/Description: The hypertrophic cardiomyopathy-associated mutant D145E, in cardiac troponin C (cTnC) C-domain, causes generalised instability at multiple sites in the isolated protein. As a result, structure and function of the mutant are more susceptible to higher temperatures. Above 25 °C there are large, progressive increases in N-domain Ca(2+)-binding affinity for D145E but only small changes for the wild-type protein. NMR-derived backbone amide temperature coefficients for many residues show a sharp...
Show moreThe hypertrophic cardiomyopathy-associated mutant D145E, in cardiac troponin C (cTnC) C-domain, causes generalised instability at multiple sites in the isolated protein. As a result, structure and function of the mutant are more susceptible to higher temperatures. Above 25 °C there are large, progressive increases in N-domain Ca(2+)-binding affinity for D145E but only small changes for the wild-type protein. NMR-derived backbone amide temperature coefficients for many residues show a sharp transition above 30-40 °C, indicating a temperature-dependent conformational change that is most prominent around the mutated EF-hand IV, as well as throughout the C-domain. Smaller, isolated changes occur in the N-domain. Cardiac skinned fibres reconstituted with D145E are more sensitive to Ca(2+) than fibres reconstituted with wild-type, and this defect is amplified near body-temperature. We speculate that the D145E mutation destabilises the native conformation of EF-hand IV, leading to a transient unfolding and dissociation of helix H that becomes more prominent at higher temperatures. This creates exposed hydrophobic surfaces that may be capable of binding unnaturally to a variety of targets, possibly including the N-domain of cTnC when it is in its open Ca(2+)-saturated state. This would constitute a potential route for propagating signals from one end of TnC to the other.
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Date Issued: 2017-04-06
Identifier: FSU_pmch_28386062, 10.1038/s41598-017-00777-6, PMC5429600, 28386062, 28386062, 10.1038/s41598-017-00777-6
Format: Citation

Title: Amount of Time in Print Reading in General Education Kindergarten Classrooms: What Does It Look Like for Students At-risk for Reading Difficulties?.
Creator: Kent, Shawn C, Wanzek, Jeanne, Al Otaiba, Stephanie
Abstract/Description: The purpose of this study was to examine the amount of time spent actively engaged in reading sounds, words, and connected text for students at-risk for reading difficulties in the first formal grade of reading instruction, kindergarten. Observational data of 109 kindergarten students at high-risk for later reading difficulties were collected during general education reading instruction across the school year. Findings revealed students read orally for just over 1 minute during their reading...
Show moreThe purpose of this study was to examine the amount of time spent actively engaged in reading sounds, words, and connected text for students at-risk for reading difficulties in the first formal grade of reading instruction, kindergarten. Observational data of 109 kindergarten students at high-risk for later reading difficulties were collected during general education reading instruction across the school year. Findings revealed students read orally for just over 1 minute during their reading instruction with approximately equal time spent reading sounds, words, or connected text. Implications of these results for early reading instruction and intervention for students at-risk for reading difficulties or disabilities are presented.
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Date Issued: 2012-05-01
Identifier: FSU_pmch_23087545, 10.1111/j.1540-5826.2012.00351.x, PMC3475198, 23087545, 23087545
Format: Citation

Title: Allosteric Activation Of Senp1 By Sumo1 Beta-grasp Domain Involves A Dock-and-coalesce Mechanism.
Creator: Guo, Jingjing, Zhou, Huan-Xiang
Abstract/Description: Small ubiquitin-related modifiers (SUMOs) are conjugated to proteins to regulate a variety of cellular processes. SENPs are cysteine proteases with a catalytic center located within a channel between two subdomains that catalyzes SUMO C-terminal cleavage for processing of SUMO precursors and de-SUMOylation of target proteins. The beta-grasp domain of SUMOs binds to an exosite cleft, and allosterically activates SENPs via an unknown mechanism. Our molecular dynamics simulations showed that...
Show moreSmall ubiquitin-related modifiers (SUMOs) are conjugated to proteins to regulate a variety of cellular processes. SENPs are cysteine proteases with a catalytic center located within a channel between two subdomains that catalyzes SUMO C-terminal cleavage for processing of SUMO precursors and de-SUMOylation of target proteins. The beta-grasp domain of SUMOs binds to an exosite cleft, and allosterically activates SENPs via an unknown mechanism. Our molecular dynamics simulations showed that binding of the beta-grasp domain induces significant conformational and dynamic changes in SENP1, including widening of the exosite cleft and quenching of nanosecond dynamics in all but a distal region. A dock-and-coalesce mechanism emerges for SENP-catalyzed SUMO cleavage: the wedging of the beta-grasp domain enables the docking of the proximal portion of the C-terminus and the strengthened cross-channel motional coupling initiates inter-subdomain correlated motions to allow for the distal portion to coalesce around the catalytic center.
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Date Issued: 2016-08-31
Identifier: FSU_libsubv1_wos_000384449900001, 10.7554/eLife.18249
Format: Citation

Title: Alternative Folding Nuclei Definitions Facilitate the Evolution of a Symmetric Protein Fold from a Smaller Peptide Motif.
Creator: Longo, Liam, Lee, Jihun, Tenorio, Connie, Blaber, Michael
Abstract/Description: Protein 3° structure symmetry is a defining feature of nearly a third of protein folds and is generally thought to result from a combination of gene duplication, fusion, and truncation events. Such events represent major replication errors, involving substantial alteration of protein 3° structure as well as causing regions of exact repeating 1° structure, both of which are generally considered deleterious to protein folding. Thus, the prevalence of symmetric protein folds is counterintuitive...
Show moreProtein 3° structure symmetry is a defining feature of nearly a third of protein folds and is generally thought to result from a combination of gene duplication, fusion, and truncation events. Such events represent major replication errors, involving substantial alteration of protein 3° structure as well as causing regions of exact repeating 1° structure, both of which are generally considered deleterious to protein folding. Thus, the prevalence of symmetric protein folds is counterintuitive and suggests a specific, yet unexplained, robustness. Using a designed β-trefoil protein, we show that purely symmetric 1° structure enables utilization of alternative definitions of the critical folding nucleus in response to gross structural rearrangement. Thus, major replication errors producing 1° structure symmetry can conserve foldability. The results provide an explanation for the prevalence of symmetric protein folds, and highlight a critical role for 1° structure symmetry in protein evolution.
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Date Issued: 2013-10-17
Identifier: FSU_libsubv1_scholarship_submission_1456501539, 10.1016/j.str.2013.09.003
Format: Citation

Title: The Impact of Li Grain Size on Coulombic Efficiency in Li Batteries.
Creator: Mehdi, B Layla, Stevens, Andrew, Qian, Jiangfeng, Park, Chiwoo, Xu, Wu, Henderson, Wesley A, Zhang, Ji-Guang, Mueller, Karl T, Browning, Nigel D
Abstract/Description: One of the most promising means to increase the energy density of state-of-the-art lithium Li-ion batteries is to replace the graphite anode with a Li metal anode. While the direct use of Li metal may be highly advantageous, at present its practical application is limited by issues related to dendrite growth and low Coulombic efficiency, CE. Here operando electrochemical scanning transmission electron microscopy (STEM) is used to directly image the deposition/stripping of Li at the anode...
Show moreOne of the most promising means to increase the energy density of state-of-the-art lithium Li-ion batteries is to replace the graphite anode with a Li metal anode. While the direct use of Li metal may be highly advantageous, at present its practical application is limited by issues related to dendrite growth and low Coulombic efficiency, CE. Here operando electrochemical scanning transmission electron microscopy (STEM) is used to directly image the deposition/stripping of Li at the anode-electrolyte interface in a Li-based battery. A non-aqueous electrolyte containing small amounts of HO as an additive results in remarkably different deposition/stripping properties as compared to the "dry" electrolyte when operated under identical electrochemical conditions. The electrolyte with the additive deposits more Li during the first cycle, with the grain sizes of the Li deposits being significantly larger and more variable. The stripping of the Li upon discharge is also more complete, i.e., there is a higher cycling CE. This suggests that larger grain sizes are indicative of better performance by leading to more uniform Li deposition and an overall decrease in the formation of Li dendrites and side reactions with electrolyte components, thus potentially paving the way for the direct use of Li metal in battery technologies.
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Date Issued: 2016-10-05
Identifier: FSU_pmch_27703188, 10.1038/srep34267, PMC5050435, 27703188, 27703188, srep34267
Format: Citation

Title: Semiclosed Conformations of the Ligand-Binding Domains of NMDA Receptors during Stationary Gating.
Creator: Dai, Jian, Zhou, Huan-Xiang
Abstract/Description: NMDA receptors are tetrameric ligand-gated ion channels. In the continuous presence of saturating agonists, NMDA receptors undergo stationary gating, in which the channel stochastically switches between an open state that permits ion conductance and a closed state that prevents permeation. The ligand-binding domains (LBDs) of the four subunits are expected to have closed clefts in the channel-open state. On the other hand, there is little knowledge about the conformational status of the LBDs...
Show moreNMDA receptors are tetrameric ligand-gated ion channels. In the continuous presence of saturating agonists, NMDA receptors undergo stationary gating, in which the channel stochastically switches between an open state that permits ion conductance and a closed state that prevents permeation. The ligand-binding domains (LBDs) of the four subunits are expected to have closed clefts in the channel-open state. On the other hand, there is little knowledge about the conformational status of the LBDs in the channel-closed state during stationary gating. To probe the latter conformational status, Kussius and Popescu engineered interlobe disulfide cross-links in NMDA receptors and found that the cross-linking produced stationary gating kinetics that differed only subtly from that produced by agonist binding. These authors assumed that the cross-linking immobilized the LBDs in cleft-closed conformations, and consequently concluded that throughout stationary gating, agonist-bound LBDs also stayed predominantly in cleft-closed conformations and made only infrequent excursions to cleft-open conformations. Here, by calculating the conformational free energies of cross-linked and agonist-bound LBDs, we assess whether cross-linking actually traps the LBDs in cleft-closed conformations and delineate semiclosed conformations of agonist-bound LBDs that may potentially be thermodynamically and kinetically important during stationary gating. Our free-energy results show that the cross-linked LBDs are not locked in the fully closed form; rather, they sample semiclosed conformations almost as readily as the agonist-bound LBDs. Several lines of reasoning suggest that LBDs are semiclosed in the channel-closed state during stationary gating. Our free-energy simulations suggest possible structural details of such semiclosed LBD conformations, including intra- and intermolecular interactions that serve as alternatives to those in the cleft-closed conformations.
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Date Issued: 2016-10-04
Identifier: FSU_pmch_27705765, 10.1016/j.bpj.2016.08.010, PMC5052434, 27705765, 27705765, S0006-3495(16)30697-X
Format: Citation

Title: Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life.
Creator: Hamilton, Chris A, Lemmon, Alan R, Lemmon, Emily Moriarty, Bond, Jason E
Abstract/Description: Despite considerable effort, progress in spider molecular systematics has lagged behind many other comparable arthropod groups, thereby hindering family-level resolution, classification, and testing of important macroevolutionary hypotheses. Recently, alternative targeted sequence capture techniques have provided molecular systematics a powerful tool for resolving relationships across the Tree of Life. One of these approaches, Anchored Hybrid Enrichment (AHE), is designed to recover hundreds...
Show moreDespite considerable effort, progress in spider molecular systematics has lagged behind many other comparable arthropod groups, thereby hindering family-level resolution, classification, and testing of important macroevolutionary hypotheses. Recently, alternative targeted sequence capture techniques have provided molecular systematics a powerful tool for resolving relationships across the Tree of Life. One of these approaches, Anchored Hybrid Enrichment (AHE), is designed to recover hundreds of unique orthologous loci from across the genome, for resolving both shallow and deep-scale evolutionary relationships within non-model systems. Herein we present a modification of the AHE approach that expands its use for application in spiders, with a particular emphasis on the infraorder Mygalomorphae. Our aim was to design a set of probes that effectively capture loci informative at a diversity of phylogenetic timescales. Following identification of putative arthropod-wide loci, we utilized homologous transcriptome sequences from 17 species across all spiders to identify exon boundaries. Conserved regions with variable flanking regions were then sought across the tick genome, three published araneomorph spider genomes, and raw genomic reads of two mygalomorph taxa. Following development of the 585 target loci in the Spider Probe Kit, we applied AHE across three taxonomic depths to evaluate performance: deep-level spider family relationships (33 taxa, 327 loci); family and generic relationships within the mygalomorph family Euctenizidae (25 taxa, 403 loci); and species relationships in the North American tarantula genus Aphonopelma (83 taxa, 581 loci). At the deepest level, all three major spider lineages (the Mesothelae, Mygalomorphae, and Araneomorphae) were supported with high bootstrap support. Strong support was also found throughout the Euctenizidae, including generic relationships within the family and species relationships within the genus Aptostichus. As in the Euctenizidae, virtually identical topologies were inferred with high support throughout Aphonopelma. The Spider Probe Kit, the first implementation of AHE methodology in Class Arachnida, holds great promise for gathering the types and quantities of molecular data needed to accelerate an understanding of the spider Tree of Life by providing a mechanism whereby different researchers can confidently and effectively use the same loci for independent projects, yet allowing synthesis of data across independent research groups.
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Date Issued: 2016-10-13
Identifier: FSU_pmch_27733110, 10.1186/s12862-016-0769-y, PMC5062932, 27733110, 27733110, 10.1186/s12862-016-0769-y
Format: Citation

Title: Psychometric evaluation of the Barriers to Cessation Scale.
Creator: Garey, Lorra, Jardin, Charles, Kauffman, Brooke Y, Sharp, Carla, Neighbors, Clayton, Schmidt, Norman B, Zvolensky, Michael J
Abstract/Description: The Barriers to Cessation Scale (BCS; Macnee & Talsma, 1995a) was developed to assess global and specific perceived barriers that may interfere with the quit process. Although the BCS is widely used in the literature, little scientific work has been devoted to examining the psychometric properties of the measure. Thus, the present study sought to address this gap by evaluating the BCS in a sample of 497 treatment-seeking smokers. The current study examined the factor structure of the BCS,...
Show moreThe Barriers to Cessation Scale (BCS; Macnee & Talsma, 1995a) was developed to assess global and specific perceived barriers that may interfere with the quit process. Although the BCS is widely used in the literature, little scientific work has been devoted to examining the psychometric properties of the measure. Thus, the present study sought to address this gap by evaluating the BCS in a sample of 497 treatment-seeking smokers. The current study examined the factor structure of the BCS, measurement invariance of the BCS subscales across sex and over 2 time points, and evaluated construct validity. Results indicated that the BCS was best modeled by a higher order factor structure wherein the originally proposed 3-factor solution (Addiction, External, and Internal) constituted the lower order and a global factor constituted the higher order factor. The higher order BCS structure demonstrated partial measurement invariance across sex and full measurement invariance from baseline to quit day among treatment seeking smokers. Additionally, expected relations were observed between the BCS subscales and similar and divergent constructs, and predictive validity was partially supported. The current findings provide novel empirical evidence that the BCS is a reliable measure of perceived barriers to smoking cessation across multiple domains and is related to several affective and smoking processes the may interfere with the process of quitting. (PsycINFO Database Record
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Date Issued: 2017-07-01
Identifier: FSU_pmch_27736128, 10.1037/pas0000379, PMC5311030, 27736128, 27736128, 2016-39461-001
Format: Citation

Title: Pressure induced elastic softening in framework aluminosilicate- albite (NaAlSiO).
Creator: Mookherjee, Mainak, Mainprice, David, Maheshwari, Ketan, Heinonen, Olle, Patel, Dhenu, Hariharan, Anant
Abstract/Description: Albite (NaAlSiO) is an aluminosilicate mineral. Its crystal structure consists of 3-D framework of Al and Si tetrahedral units. We have used Density Functional Theory to investigate the high-pressure behavior of the crystal structure and how it affects the elasticity of albite. Our results indicate elastic softening between 6-8 GPa. This is observed in all the individual elastic stiffness components. Our analysis indicates that the softening is due to the response of the three-dimensional...
Show moreAlbite (NaAlSiO) is an aluminosilicate mineral. Its crystal structure consists of 3-D framework of Al and Si tetrahedral units. We have used Density Functional Theory to investigate the high-pressure behavior of the crystal structure and how it affects the elasticity of albite. Our results indicate elastic softening between 6-8 GPa. This is observed in all the individual elastic stiffness components. Our analysis indicates that the softening is due to the response of the three-dimensional tetrahedral framework, in particular by the pressure dependent changes in the tetrahedral tilts. At pressure <6 GPa, the PAW-GGA can be described by a Birch-Murnaghan equation of state with = 687.4 Å, = 51.7 GPa, and = 4.7. The shear modulus and its pressure derivative are = 33.7 GPa, and = 2.9. At 1 bar, the azimuthal compressional and shear wave anisotropy = 42.8%, and = 50.1%. We also investigate the densification of albite to a mixture of jadeite and quartz. The transformation is likely to cause a discontinuity in density, compressional, and shear wave velocity across the crust and mantle. This could partially account for the Mohorovicic discontinuity in thickened continental crustal regions.
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Date Issued: 2016-10-13
Identifier: FSU_pmch_27734903, 10.1038/srep34815, PMC5062091, 27734903, 27734903, srep34815
Format: Citation

Title: Commentary: Epigenetic Regulation of Phosphodiesterases 2A and 3A Underlies Compromised β-Adrenergic Signaling in an iPSC Model of Dilated Cardiomyopathy..
Creator: Cole, Lauren A, Dennis, Jonathan H, Chase, P Bryant
Date Issued: 2016-09-23
Identifier: FSU_pmch_27721795, 10.3389/fphys.2016.00418, PMC5033966, 27721795, 27721795
Format: Citation

Title: A MULTISCALE IMPLEMENTATION BASED ON ADAPTIVE MESH REFINEMENT FOR THE NONLOCAL PERIDYNAMICS MODEL IN ONE DIMENSION.
Creator: Xu, Feifei, Gunzburger, Max, Burkardt, John, Du, Qiang
Abstract/Description: Peridynamics models for solid mechanics feature a horizon parameter (5 that specifies the maximum extent of nonlocal interactions. In this paper, a multiscale implementation of peridynamics models is proposed. In regions in which the displacement field is smooth, grid sizes are large relative to delta, leading to a local behavior of the models, whereas in regions containing defects, e.g., cracks, delta is larger than the grid size. Discontinuous (continuous) Galerkin finite element...
Show morePeridynamics models for solid mechanics feature a horizon parameter (5 that specifies the maximum extent of nonlocal interactions. In this paper, a multiscale implementation of peridynamics models is proposed. In regions in which the displacement field is smooth, grid sizes are large relative to delta, leading to a local behavior of the models, whereas in regions containing defects, e.g., cracks, delta is larger than the grid size. Discontinuous (continuous) Galerkin finite element discretizations are used in regions where defects do (do not) occur. Moreover, in regions where no defects occur, the multiscale implementation seamlessly transitions to the use of a standard finite element discretization of a corresponding PDE model. Here, we demonstrate the multiscale implementation in a simple one-dimensional setting. An adaptive strategy is incorporated to detect discontinuities and effect grid refinement, resulting in a highly accurate and efficient implementation of peridynamics.
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Date Issued: 2016
Identifier: FSU_libsubv1_wos_000373366500014, 10.1137/15M1010300
Format: Citation

Title: A framework to quantify uncertainty in simulations of oil transport in the ocean.
Creator: Goncalves, Rafael C., Iskandarani, Mohamed, Srinivasan, Ashwanth, Thacker, W. Carlisle, Chassignet, Eric, Knio, Omar M.
Abstract/Description: An uncertainty quantification framework is developed for the DeepC Oil Model based on a nonintrusive polynomial chaos method. This allows the model's output to be presented in a probabilistic framework so that the model's predictions reflect the uncertainty in the model's input data. The new capability is illustrated by simulating the far-field dispersal of oil in a Deepwater Horizon blowout scenario. The uncertain input consisted of ocean current and oil droplet size data and the main model...
Show moreAn uncertainty quantification framework is developed for the DeepC Oil Model based on a nonintrusive polynomial chaos method. This allows the model's output to be presented in a probabilistic framework so that the model's predictions reflect the uncertainty in the model's input data. The new capability is illustrated by simulating the far-field dispersal of oil in a Deepwater Horizon blowout scenario. The uncertain input consisted of ocean current and oil droplet size data and the main model output analyzed is the ensuing oil concentration in the Gulf of Mexico. A 1331 member ensemble was used to construct a surrogate for the model which was then mined for statistical information. The mean and standard deviations in the oil concentration were calculated for up to 30 days, and the total contribution of each input parameter to the model's uncertainty was quantified at different depths. Also, probability density functions of oil concentration were constructed by sampling the surrogate and used to elaborate probabilistic hazard maps of oil impact. The performance of the surrogate was constantly monitored in order to demarcate the spacetime zones where its estimates are reliable.
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Date Issued: 2016-04
Identifier: FSU_libsubv1_wos_000383462300002, 10.1002/2015JC011311
Format: Citation

Title: A dialogue about the U.S. dialogue on mental health: Exploring the nature, scope, and implications of the conversation.
Creator: Hess, Jacob Z., Decker, Anna, Lacasse, Jeffrey Raymond, Foster, Mark
Abstract/Description: With all the emphasis on having more conversation about mental health, remarkably little attention has been paid to how exactly we want to be having it. In what follows, we review five areas about which sharp differences currently exist: (1) Biology – how are physiological contributors to mental distress being framed? (2) Symptoms – how should we best work with distressing signs in the body? (3) Action Steps – what needs to happen in order to improve mental health in the nation? (4) Barriers ...
Show moreWith all the emphasis on having more conversation about mental health, remarkably little attention has been paid to how exactly we want to be having it. In what follows, we review five areas about which sharp differences currently exist: (1) Biology – how are physiological contributors to mental distress being framed? (2) Symptoms – how should we best work with distressing signs in the body? (3) Action Steps – what needs to happen in order to improve mental health in the nation? (4) Barriers – what are the primary barriers that need to be overcome in improving public mental health? (5) Lifestyle – what role do lifestyle choices play in mental health? After illustrating the different positions being taken on each of these questions, we then consider the diverging implications for individuals and families facing these problems. We conclude with recommendations as to how the larger dialogue on mental health could become more inclusive and productive.
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Date Issued: 2017-06-01
Identifier: FSU_libsubv1_scholarship_submission_1490548063
Format: Citation

Title: A Muts Beta-dependent Contribution Of Muts Alpha To Repeat Expansions In Fragile X Premutation Mice?.
Creator: Zhao, Xiao-Nan, Lokanga, Rachel, Allette, Kimaada, Gazy, Inbal, Wu, Di, Usdin, Karen
Abstract/Description: The fragile X-related disorders result from expansion of a CGG/CCG microsatellite in the 5' UTR of the FMR1 gene. We have previously demonstrated that the MSH2/MSH3 complex, MutS beta, that is important for mismatch repair, is essential for almost all expansions in a mouse model of these disorders. Here we show that the MSH2/MSH6 complex, MutS alpha also contributes to the production of both germ line and somatic expansions as evidenced by the reduction in the number of expansions observed in...
Show moreThe fragile X-related disorders result from expansion of a CGG/CCG microsatellite in the 5' UTR of the FMR1 gene. We have previously demonstrated that the MSH2/MSH3 complex, MutS beta, that is important for mismatch repair, is essential for almost all expansions in a mouse model of these disorders. Here we show that the MSH2/MSH6 complex, MutS alpha also contributes to the production of both germ line and somatic expansions as evidenced by the reduction in the number of expansions observed in Msh6(-/-) mice. This effect is not mediated via an indirect effect of the loss of MSH6 on the level of MSH3. However, since MutS beta is required for 98% of germ line expansions and almost all somatic ones, MutS alpha is apparently not able to efficiently substitute for MutS beta in the expansion process. Using purified human proteins we demonstrate that MutS alpha, like MutS beta, binds to substrates with loop-outs of the repeats and increases the thermal stability of the structures that they form. We also show that MutS alpha facilitates binding of MutS beta to these loop-outs. These data suggest possible models for the contribution of MutS alpha to repeat expansion. In addition, we show that unlike MutS beta, MutS alpha may also act to protect against repeat contractions in the Fmr1 gene.
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Date Issued: 2016-07
Identifier: FSU_libsubv1_wos_000381050100049, 10.1371/journal.pgen.1006190
Format: Citation

Title: A flexible iron(II) complex in which zero-field splitting is resistant to structural variation.
Creator: Zadrozny, Joseph M., Greer, Samuel M., Hill, Stephen, Freedman, Danna E.
Abstract/Description: The relationship between electronic structure and zero-field splitting dictates key design parameters for magnetic molecules. In particular, to enable the directed synthesis of new electronic spin based qubits, developing complexes where zero-field splitting energies are invariant to structural changes is a critical challenge. Toward those ends, we report three salts of a new compound, a four-coordinate iron(II) complex [ Fe(C3S5)(2)](2-) ([(18-crown-6) K](+) (1), Ph4P+ (2), Bu4N+ (3)) with a...
Show moreThe relationship between electronic structure and zero-field splitting dictates key design parameters for magnetic molecules. In particular, to enable the directed synthesis of new electronic spin based qubits, developing complexes where zero-field splitting energies are invariant to structural changes is a critical challenge. Toward those ends, we report three salts of a new compound, a four-coordinate iron(II) complex [ Fe(C3S5)(2)](2-) ([(18-crown-6) K](+) (1), Ph4P+ (2), Bu4N+ (3)) with a continuous structural variation in a single parameter, the dihedral angle (theta(d)) between the two C3S52- ligands, as a function of counterion (theta(d) = 89.98(4)degrees for 1 to 72.41(2)degrees for 3). Electron paramagnetic resonance data for 1-3 reveal zero-field splitting parameters that are unusually robust to the structural variation. Mossbauer spectroscopic measurements indicate that the structural variation in theta(d) primarily affects the highest-energy 3d-orbitals (d(xz) and d(yz)) of the iron(II) ion. These orbitals have the smallest impact on the zero-field splitting parameters, thus the distortion has a minor effect on D and E. These results represent the first part of a directed effort to understand how spin state energies may be fortified against structural distortions for future applications of qubits in non-crystalline environments.
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Date Issued: 2016
Identifier: FSU_libsubv1_wos_000366826900047, 10.1039/c5sc02477c
Format: Citation

Title: A PRIMITIVE HADROSAURID FROM SOUTHEASTERN NORTH AMERICA AND THE ORIGIN AND EARLY EVOLUTION OF 'DUCK- BILLED' DINOSAURS.
Creator: Prieto-Marquez, Albert, Erickson, Gregory M., Ebersole, Jun A.
Abstract/Description: Eotrachodon orientalis gen. et sp. nov. (latest Santonian of Alabama, southeastern U.S.A.) is one of the oldest and most basal hadrosaurid dinosaurs and the only hadrosaurid from Appalachia (present day eastern North America) with a preserved skull. This taxon possesses a relatively derived narial structure that was until now regarded as synapomorphic for saurolophine (solid-crested or crestless) hadrosaurids. Maximum parsimony analysis places E. orientalis as the sister taxon to...
Show moreEotrachodon orientalis gen. et sp. nov. (latest Santonian of Alabama, southeastern U.S.A.) is one of the oldest and most basal hadrosaurid dinosaurs and the only hadrosaurid from Appalachia (present day eastern North America) with a preserved skull. This taxon possesses a relatively derived narial structure that was until now regarded as synapomorphic for saurolophine (solid-crested or crestless) hadrosaurids. Maximum parsimony analysis places E. orientalis as the sister taxon to Saurolophidae (Saurolophinae + Lambeosaurinae). Character optimization on the phylogeny indicates that the saurolophine-like circumnarial structure evolved by the Santonian following the split between saurolophines and lambeosaurines but prior to the major hadrosaurid radiation. Statistical dispersal-vicariance analysis posits an Appalachian ancestral area for Hadrosauridae and subsequent dispersal of their ancestors into Laramidia (present-day western North America) during the Cenomanian.http://zoobank.org/urn:lsid:zoobank.org:pub:3AD914D2-A3A5-45FD-8C94-5EE80461FEBCSUPPLEMENTAL DATASupplemental materials are available for this article for free at www.tandfonline.com/UJVPCitation for this article: Prieto-Marquez, A., G. M. Erickson, and J. A. Ebersole. 2016. A primitive hadrosaurid from southeastern North America and the origin and early evolution of duck-billed' dinosaurs. Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2015.1054495
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Date Issued: 2016-03-03
Identifier: FSU_libsubv1_wos_000372953300019, 10.1080/02724634.2015.1054495
Format: Citation

Title: Acupoint Sensitization, Acupuncture Analgesia, Acupuncture on Visceral Functional Disorders, and Its Mechanism.
Creator: Yu, Xiaochun, Zhu, Bing, Lin, Zhixiu, Qiao, Haifa, Kong, Jian, Gao, Xinyan
Date Issued: 2015-01-01
Identifier: FSU_pmch_26300944, 10.1155/2015/171759, PMC4537726, 26300944, 26300944
Format: Citation

Title: Acute BDNF treatment upregulates GluR1-SAP97 and GluR2-GRIP1 interactions: implications for sustained AMPA receptor expression..
Creator: Jourdi, Hussam, Kabbaj, Mohamed
Abstract/Description: Brain-derived neurotrophic factor (BDNF) plays several prominent roles in synaptic plasticity and in learning and memory formation. Reduced BDNF levels and altered BDNF signaling have been reported in several brain diseases and behavioral disorders, which also exhibit reduced levels of AMPAr subunits. BDNF treatment acutely regulates AMPA receptor expression and function, including synaptic AMPAr subunit trafficking, and implicates several well defined signaling molecules that are required to...
Show moreBrain-derived neurotrophic factor (BDNF) plays several prominent roles in synaptic plasticity and in learning and memory formation. Reduced BDNF levels and altered BDNF signaling have been reported in several brain diseases and behavioral disorders, which also exhibit reduced levels of AMPAr subunits. BDNF treatment acutely regulates AMPA receptor expression and function, including synaptic AMPAr subunit trafficking, and implicates several well defined signaling molecules that are required to elicit long term potentiation and depression (LTP and LTD, respectively). Long term encoding of synaptic events, as in long term memory formation, requires AMPAr stabilization and maintenance. However, factors regulating AMPAr stabilization in neuronal cell membranes and synaptic sites are not well characterized. In this study, we examine the effects of acute BDNF treatment on levels of AMPAr-associated scaffolding proteins and on AMPAr subunit-scaffolding protein interactions. We also examine the effects of BDNF-dependent enhanced interactions between AMPAr subunits with their specific scaffolding proteins on the accumulation of both types of proteins. Our results show that acute BDNF treatment upregulates the interactions between AMPAr subunits (GluR1 and GluR2) with their scaffold proteins SAP97 and GRIP1, respectively, leading to prolonged increased accumulation of both categories of proteins, albeit with distinct mechanisms for GluR1 and GluR2. Our findings reveal a new role for BDNF in the long term maintenance of AMPA receptor subunits and associated scaffolding proteins at synapses and further support the role of BDNF as a key regulator of synaptic consolidation. These results have potential implications for recent findings implicating BDNF and AMPAr subunits in various brain diseases and behavioral disorders.
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Date Issued: 2013-01-01
Identifier: FSU_pmch_23460828, 10.1371/journal.pone.0057124, PMC3584105, 23460828, 23460828, PONE-D-12-38051
Format: Citation

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