Search results
(1 - 50 of 381)
Pages
- Title
- A Locus Affecting Retinal Lamination and Synaptic Formation in Danio Rerio.
- Creator
-
Benoit, Chelsea, Fadool, James Michael, Levenson, Cathy W., Keller, Laura R., Yu, Hong-Guo, Florida State University, College of Arts and Sciences, Department of Biological Science
- Abstract/Description
-
The structure and function of the eye is conserved across vertebrates. Mutagenesis screens of zebrafish have led to the discovery of many genes essential for retinal development and function. In one such screen, a recessive allele on the zvm9 locus was identified due to the lack of an optokinetic reflex in larvae of otherwise normal appearance. Preliminary histology indicated a lack of lamination in the zvm9 mutant retina and immunohistochemistry confirmed phenotypic defects in all layers of...
Show moreThe structure and function of the eye is conserved across vertebrates. Mutagenesis screens of zebrafish have led to the discovery of many genes essential for retinal development and function. In one such screen, a recessive allele on the zvm9 locus was identified due to the lack of an optokinetic reflex in larvae of otherwise normal appearance. Preliminary histology indicated a lack of lamination in the zvm9 mutant retina and immunohistochemistry confirmed phenotypic defects in all layers of the retina. These included thin or absent plexiform layers, disorganization of cells within laminae, and the loss of specific retinal cell types. Additionally, cell death in both the retina and forebrain was identified by TUNEL. The presence of ectopic mitoses during retinal development and the later observation of ectopic cones suggests that the zvm9 product is a crucial component of a complex regulating cell-cell junctions or apical-basal polarity. The notable absence of synaptic structures in cone photoreceptors is consistent with my hypothesis that this complex is also required for proper cell-cell interactions.
Show less - Date Issued
- 2014
- Identifier
- FSU_migr_etd-9140
- Format
- Thesis
- Title
- Determining the Genetic Network of Primary Microcephaly Disease (MCPH).
- Creator
-
Dietrick, Barbara, Department of Biological Science
- Abstract/Description
-
The developmental disorder, autosomal recessive primary microcephaly (MCPH), results in reduced cerebral cortex growth during fetal development. Mutations in 9 centrosome protein-encoding genes characterize this genetically heterogeneous disease. We used centrosomin (cnn) mutant Drosophila as the model to genetically dissect the disease pathway. Centrosome assembly and microtubule-organizing regulation requires cnn, but cnn mutant adults survive with undefined neuropathology, presumably...
Show moreThe developmental disorder, autosomal recessive primary microcephaly (MCPH), results in reduced cerebral cortex growth during fetal development. Mutations in 9 centrosome protein-encoding genes characterize this genetically heterogeneous disease. We used centrosomin (cnn) mutant Drosophila as the model to genetically dissect the disease pathway. Centrosome assembly and microtubule-organizing regulation requires cnn, but cnn mutant adults survive with undefined neuropathology, presumably related to MCPH pathology. To discover the basis of this pathology, we used an RNA interference screen to identify cnn mutant modifiers. From this approach, we discovered that genes controlling autophagy and microtubule regulation strongly enhanced cnn. Two components of the augmin complex, an eight subunit complex that regulates microtubules during mitosis, were identified in the screens. I investigated one of these augmin subunit mutants, a mutation in dim gamma tubulin 4 (dgt41), and found neuroblasts with impaired microtubule organization and reduced Cnn and gamma-tubulin centrosomal recruitment. The dgt41 mutant shows an early embryo lethality with severe microtubule-organizational defects, including a lack of microtubules attached to kinetochores. These findings suggest microtubule organization and autophagy play critical roles in MCPH. Because autophagy requires cnn and microtubules, and the combination of dgt4 and cnn mutations is lethal, I hypothesize that Dgt4, and the augmin complex, also regulate autophagy. This would be an entirely new function for this conserved protein complex. My project tests this novel concept and contributes valuable information about autophagy regulation and MCPH mechanisms.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0454
- Format
- Thesis
- Title
- ORIGIN OF DNA REPLICATION IN XENOPUS LAEVIS: CLONING OF ORIGIN AND ITS CHARACTERIZATION.
- Creator
-
WATANABE, SHINICHI., Florida State University
- Date Issued
- 1980, 1980
- Identifier
- AAI8100655, 3084748, FSDT3084748, fsu:74249
- Format
- Document (PDF)
- Title
- METHYLATION PATTERNS IN BOVINE SATELLITE I DNA.
- Creator
-
MCGRAW, ROYAL ALFRED, III., Florida State University
- Abstract/Description
-
Methylation patterns in bovine satellite I DNA were studied by direct sequence analysis. Satellite I DNA was prepared as a discrete restriction fragment from genomic DNA of various bovine tissues and subjected to sequencing by the technique of Maxam and Gilbert. Sequence was obtained for a region of 350 nucleotide pairs in the satellite fragments from calf thymus and bull sperm DNA's. Comparison of sequence ladders from methylated and unmethylated DNA's (thymus and sperm, respectively)...
Show moreMethylation patterns in bovine satellite I DNA were studied by direct sequence analysis. Satellite I DNA was prepared as a discrete restriction fragment from genomic DNA of various bovine tissues and subjected to sequencing by the technique of Maxam and Gilbert. Sequence was obtained for a region of 350 nucleotide pairs in the satellite fragments from calf thymus and bull sperm DNA's. Comparison of sequence ladders from methylated and unmethylated DNA's (thymus and sperm, respectively) facilitated identification of methylated cytosines., Methylation in thymus DNA was shown to be confined to the configuration 5' ('m)CpG 3', and there was no indication that these sites were methylated in the sperm sequence. In thymus DNA, there were a few occurrences of the CpG doublet in which cytosines were apparently not methylated. In regions where sequence was obtained for both strands, methylation was shown to be symmetrical. When the analysis was extended to DNA from other tissues, the unmethylated pattern was demonstrated only in chorion DNA. All other tissues showed the thymus methylation pattern., Sequence was also obtained from a cloned satellite I fragment. This sequence was compared by computer with sequence from genomic DNA and with other known bovine satellites. A computerized homology search demonstrated degenerate repetitions within the satellite I sequence and degenerate homologies with other bovine satellites., The results are discussed in light of other recent findings, and in terms of their possible implications for evolution and development.
Show less - Date Issued
- 1982, 1982
- Identifier
- AAI8212898, 3085227, FSDT3085227, fsu:74722
- Format
- Document (PDF)
- Title
- ALTERATIONS IN FINE STRUCTURE DURING EMBRYO AND ENDOSPERM DEVELOPMENT IN LETHAL HYBRIDS INVOLVING HIBISCUS COSTATUS.
- Creator
-
ASHLEY, TERRY., The Florida State University
- Date Issued
- 1970, 1970
- Identifier
- AAI7106958, 2986367, FSDT2986367, fsu:70876
- Format
- Document (PDF)
- Title
- REGULATION OF THE BIOSYNTHESIS AND UTILIZATION OF FREE AROMATIC AMINO ACIDS IN NEUROSPORA CRASSA.
- Creator
-
BROOKS, CAROLYN JEAN FREECE., The Florida State University
- Date Issued
- 1969, 1969
- Identifier
- AAI7016320, 2986193, FSDT2986193, fsu:70702
- Format
- Document (PDF)
- Title
- AMINO ACID INCORPORATION IN AN IN VITRO SYSTEM OF NEUROSPORA CRASSA.
- Creator
-
LEE, PING CHEUNG., The Florida State University
- Date Issued
- 1968, 1968
- Identifier
- AAI6913274, 2985940, FSDT2985940, fsu:70449
- Format
- Document (PDF)
- Title
- GLUCAMYLASE OF NEUROSPORA: A REGULATED EXOENZYME.
- Creator
-
FASS, DAVID NEAL., The Florida State University
- Date Issued
- 1969, 1969
- Identifier
- AAI7011113, 2986131, FSDT2986131, fsu:70640
- Format
- Document (PDF)
- Title
- ISOLATION AND CHARACTERIZATION OF EARLY-REPLICATING AND LATE-REPLICATING DNA OF THE CHINESE HAMSTER.
- Creator
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MYERS, TERRY LEWIS., The Florida State University
- Date Issued
- 1969, 1969
- Identifier
- AAI7016342, 2986198, FSDT2986198, fsu:70707
- Format
- Document (PDF)
- Title
- STRUCTURAL SUBUNITS OF CHINESE HAMSTER DNA DURING TRANSCRIPTION AND REPLICATION.
- Creator
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MEGO, WILLIAM AUSTIN., The Florida State University
- Date Issued
- 1970, 1970
- Identifier
- AAI7107065, 2986399, FSDT2986399, fsu:70908
- Format
- Document (PDF)
- Title
- MULTIPLE FORMS OF BETA-GALACTOSIDASE IN NEUROSPORA CRASSA.
- Creator
-
JOHNSON, HARRY NELS., The Florida State University
- Date Issued
- 1969, 1969
- Identifier
- AAI6917675, 2985991, FSDT2985991, fsu:70500
- Format
- Document (PDF)
- Title
- DNA REPAIR IN HAEMOPHILUS INFLUENZAE.
- Creator
-
BAGCI, HASAN., The Florida State University
- Date Issued
- 1979, 1979
- Identifier
- AAI8006222, 2989406, FSDT2989406, fsu:73913
- Format
- Document (PDF)
- Title
- MEMBRANE FUNCTION IN CYSTIC FIBROSIS: CHARACTERIZATION OF A CYSTIC FIBROSIS FACTOR FRACTIONATED FROM FIBROBLAST MEDIA WITH RESPECT TO THE EFFLUX OF PUTRESCINE.
- Creator
-
STARNES, MARY MILDRED., The Florida State University
- Abstract/Description
-
In this study, a polycationic substance which is produced by cystic fibrosis fibroblasts and secreted into the growth medium has been identified and characterized with respect to its action on the efflux of putrescine from normal fibroblasts. Comparison of medium extracts from various cystic fibrosis homozygotes, heterozygotes and where possible normal fibroblasts from family groups have been undertaken in order to determine the relationship of this factor to the putative abnormal gene in...
Show moreIn this study, a polycationic substance which is produced by cystic fibrosis fibroblasts and secreted into the growth medium has been identified and characterized with respect to its action on the efflux of putrescine from normal fibroblasts. Comparison of medium extracts from various cystic fibrosis homozygotes, heterozygotes and where possible normal fibroblasts from family groups have been undertaken in order to determine the relationship of this factor to the putative abnormal gene in cystic fibrosis. Physiological characterization of the factor secret-by cystic fibrosis homozygotes into fibroblast medium with respect to its effect on putrescine efflux in normal fibroblasts, including comparisons with the known membrane active substance polygalactosamine, has been pursued in order to delineate the possible role of cystic fibrosis factor in the pathophysiology of the disease., The cystic fibrosis factor from medium is capable of causing the efflux of exogenously supplied putrescine. This activity is enhanced in the presence of extracellular calcium. Efflux of exogenously supplied putrescine is used as a bioassay for the cystic fibrosis factor. The putrescine efflux assay can be used as a quantitative bioassay for the cystic fibrosis factor. The response of the cystic fibrosis factor in the presence of calcium distinguishes it from polygalactosamine. The cystic fibrosis factor is found in medium from all homozygote and heterozygote cell lines tested but not in media from normal cell lines. It is proposed that the ability of the cystic fibrosis factor to alter the membrane permeability of cells and allow influx of extracellular calcium may be the underlying lesion which expresses itself phenotypically as cystic fibrosis. A model for the mode of action of the cystic fibrosis factor is presented.
Show less - Date Issued
- 1980, 1980
- Identifier
- AAI8021107, 2989668, FSDT2989668, fsu:74175
- Format
- Document (PDF)
- Title
- THE NUCLEOTIDE SEQUENCE AND EVOLUTIONARY IMPLICATIONS OF ASPARTIC ACID TRANSFER-RNA FROM THE CHLOROPLAST OF EUGLENA GRACILIS.
- Creator
-
DELEHANTY, JOHN THOMAS., Florida State University
- Abstract/Description
-
The nucleotide sequence of aspartic acid tRNA from the chloroplast of Euglena gracilis has been determined. In resembling prokaryotic tRNA, the sequence of the chloroplast tRNA('asp) represents additional evidence in support of the prokaryotic endosymbiotic theory of the origin of the chloroplast. The accuracy of this sequence determination has been buttressed by the independent use of two techniques, conventional angular mobility shift analysis and the recently developed rapid print-readout...
Show moreThe nucleotide sequence of aspartic acid tRNA from the chloroplast of Euglena gracilis has been determined. In resembling prokaryotic tRNA, the sequence of the chloroplast tRNA('asp) represents additional evidence in support of the prokaryotic endosymbiotic theory of the origin of the chloroplast. The accuracy of this sequence determination has been buttressed by the independent use of two techniques, conventional angular mobility shift analysis and the recently developed rapid print-readout method. Further, these methods have been combined in a novel approach to support the proposed sequence.
Show less - Date Issued
- 1980, 1980
- Identifier
- AAI8111927, 3084867, FSDT3084867, fsu:74368
- Format
- Document (PDF)
- Title
- A KCL EXTRACTABLE L- AMINO ACID OXIDASE FROM NEUROSPORA CRASSA.
- Creator
-
COLLIER, IVAN ELLSWORTH., Florida State University
- Abstract/Description
-
An L-amino acid oxidase is extracted from whole Neurospora crassa conidia when they are treated with saturated (4.8 M) KCL. This procedure preferentially extracts cell surface molecules, many of which are components of the amino acid transport system and leaves the cells completely viable., The L-amino acid oxidase has a molecular weight of 160,000 daltons by gel filtration chromatograhy and 85,000 daltons by SDS polyacrylamide gel electrophoresis. The oxidase is sensitive to zinc ions, and...
Show moreAn L-amino acid oxidase is extracted from whole Neurospora crassa conidia when they are treated with saturated (4.8 M) KCL. This procedure preferentially extracts cell surface molecules, many of which are components of the amino acid transport system and leaves the cells completely viable., The L-amino acid oxidase has a molecular weight of 160,000 daltons by gel filtration chromatograhy and 85,000 daltons by SDS polyacrylamide gel electrophoresis. The oxidase is sensitive to zinc ions, and not to the sulfhydryl reagent, iodoacetic acid. The neutral (N) amino acid transport system in Neurospora crassa is also sensitive to zinc ions. The similarity between the concentration dependence of zinc inhibition of the oxidase and the transport system suggests that the oxidase may be involved in the membrane-mediated transport process.
Show less - Date Issued
- 1980, 1980
- Identifier
- AAI8108384, 3084859, FSDT3084859, fsu:74360
- Format
- Document (PDF)
- Title
- BIOCHEMICAL CHARACTERIZATION OF SEROLOGICALLY-DEFINED RABBIT HEAVY CHAIN VARIABLE REGION ALLOTYPES OF THE A AND Y SUBGROUPS (IMMUNOGLOBULIN, IDIOTYPE, PEPTIDE MAPPING).
- Creator
-
ABOLHASSANI, MOHSEN., Florida State University
- Abstract/Description
-
Structural studies on the serologically-defined rabbit VHa('+) (a1, a2, and a3) and VHa('-) (y33,30 and y33,-) immunoglobulins have been performed in order to establish that these genetic markers reflect the presence of different primary gene products. In addition, biochemical studies were carried out on induced non-a2 anti-a1-reactive molecule in order to determine whether these molecules represent latent allotypes or are an internal image idiotype., Initially, allotype-defined heavy chains...
Show moreStructural studies on the serologically-defined rabbit VHa('+) (a1, a2, and a3) and VHa('-) (y33,30 and y33,-) immunoglobulins have been performed in order to establish that these genetic markers reflect the presence of different primary gene products. In addition, biochemical studies were carried out on induced non-a2 anti-a1-reactive molecule in order to determine whether these molecules represent latent allotypes or are an internal image idiotype., Initially, allotype-defined heavy chains were prepared from the affinity-purified IgG molecules. These were then subjected to tryptic digestion and were analyzed by HPLC. Approximately 38-40 distinct peptides were resolved from each heavy chain, of which about 30 peptides were derived from Fc fragment (CH2 and CH3) and 8-10 peptides were derived from Fd region (VH1 and CH1). Seven Fd peptides were shared by all VHa('+) and VHa('-) heavy chains. Each of the a1 and the a2 digests had one allotype-specific peptide (in addition to the common peptides), whereas no allotype-specific peptides were observed for a3 heavy chain. No differences were detected between y33,30 and y33,- peptides, however, both expressed a common y-specific peptide., Comparison of the nominal a1 digest with non-a2 anti-al-reactive heavy chain digest revealed that non-a2 anti-a1-reactive molecule expressed an a1-specific peptide. This observation, together with previous immunoelectron microscopic data, suggests that non-a2 anti-a1-reactive molecule are possibly latent a1 allotype., Amino acid analyses of the isolated a1 and y-specific peptides indicate that the y-specific peptide is very similar to the first 19 N-terminal amino acid residues of the previously reported pooled VHa('-) molecule (e.g., the two peptides matched at 16 residues out of 19 residues). The a1-specific peptide was very similar (except one extra amino acid) to the N-terminal 10 amino acid residues of the VHa1 molecule. These data indicate that both a1 and y-specific peptides are located in the first VH framework region.
Show less - Date Issued
- 1984, 1984
- Identifier
- AAI8505281, 3086012, FSDT3086012, fsu:75498
- Format
- Document (PDF)
- Title
- GENES OF THE RABBIT VH SUBGROUP III.
- Creator
-
LASTER, SCOTT MATTHEW., Florida State University
- Abstract/Description
-
The rabbit VH region has been the subject of extensive and continued serological and biochemical analysis during the past three decades. Four VH subgroups (a, x, y, w) have been characterized, each of which displays a distinct set of allelic products (allotypes). However, despite years of study, certain basic phenomena associated with VH allotype expression remain unexplained. For these, and other reasons we have undertaken the analysis of the rabbit VH gene complex at the level of the DNA.,...
Show moreThe rabbit VH region has been the subject of extensive and continued serological and biochemical analysis during the past three decades. Four VH subgroups (a, x, y, w) have been characterized, each of which displays a distinct set of allelic products (allotypes). However, despite years of study, certain basic phenomena associated with VH allotype expression remain unexplained. For these, and other reasons we have undertaken the analysis of the rabbit VH gene complex at the level of the DNA., In this report, we present the sequence of a rabbit VH gene (pRVH831), which was selected from a rabbit genomic library using the mouse VH gene, pS107V1, as a cross-species hybridization probe. Our analysis reveals that pRVH831 is a highly defective VH pseudogene. It contains three deletions toward the 3' end, which cause a frameshift mutation in FR3 and cripple the D region splice site., We have determined that pRVH831 is a member of the VH subgroup III. When used as a probe in filter hybridization experiments pRVH831 reveals 10-15 VH subgroup III genes, a number consistent with results obtained in human and murine systems. Furthermore, our analysis of the predicted translational product of pRVH831 confirms that at least some rabbit VHa-negative genes are members of the VH subgroup III. We have also examined genomic DNA obtained from pedigreed rabbits of different VH haplotypes. These data reveal haplotype specific hybridization patterns and support previous indications that recombination in the rabbit VH gene complex is extremely rare., When the sequence of pRVH831 was analysed for internal repetitiveness, three similar copies (73-90%) of a 14-15 bp sequence were detected. These sequences are located in FR1, CDR1, and CDR2. Our analysis indicates that this repeat is homologous to a portion of a primordial VH sequence. However, this is also the same sequence which appears in the human D minigenes and in some CDR2s. We have determined that this sequence occurs in many VH genes, T cell receptor genes, and can contain Chi and Chi-like recombination sequences from lambda phage.
Show less - Date Issued
- 1984, 1984
- Identifier
- AAI8505304, 3086020, FSDT3086020, fsu:75506
- Format
- Document (PDF)
- Title
- A comparative analysis of a major repeat DNA sequence prevalent among Anatidae (waterfowl).
- Creator
-
Madsen, Cort Stanford., Florida State University
- Abstract/Description
-
The evolution of a 190 bp tandemly organized, highly repeated DNA sequence (AMR), prevalent among Anatidae (waterflow), was extensively studied. Monomeric units of the AMR repeat family were cloned and sequenced from the American Merganser (Mergus merganser), Barrows Goldeneye (Bucephela islandica), Comb Duck (Sarkidiornis melanotos), North American Wood Duck (Aix sponsa) and Mallard (Anas platyrhynchos). Data provided from intra and interspecific sequence comparisons was analyzed with...
Show moreThe evolution of a 190 bp tandemly organized, highly repeated DNA sequence (AMR), prevalent among Anatidae (waterflow), was extensively studied. Monomeric units of the AMR repeat family were cloned and sequenced from the American Merganser (Mergus merganser), Barrows Goldeneye (Bucephela islandica), Comb Duck (Sarkidiornis melanotos), North American Wood Duck (Aix sponsa) and Mallard (Anas platyrhynchos). Data provided from intra and interspecific sequence comparisons was analyzed with respect to the modes of evolution previously proposed for other non-avian repeat families. To estimate the importance of primary DNA structure with regard to function and evolution, the AMR sequence was compared to three different nonhomologous, tandemly organized, highly repetitive DNA sequences. These repeat sequences were isolated from the Blue Fronted Amazon (Amazona aestiva), Goffins Cockatoo (Cacatua goffini) and Flamingo (Phoenicopterus indus).
Show less - Date Issued
- 1990, 1990
- Identifier
- AAI9103101, 3162121, FSDT3162121, fsu:78319
- Format
- Document (PDF)
- Title
- THE BIOSYNTHESIS OF HIGH MOLECULAR WEIGHT RIBOSOMAL AND HETEROGENEOUS RIBONUCLEIC ACID IN EUKARYOTES.
- Creator
-
MAYO, VIRGINIA SUSAN., The Florida State University
- Date Issued
- 1971, 1971
- Identifier
- AAI7213536, 2986727, FSDT2986727, fsu:71236
- Format
- Document (PDF)
- Title
- CHARACTERIZATION OF DNA FRAGMENTS RELEASED FROM REPLICATING MAMMALIAN DNA.
- Creator
-
KUREK, MICHAEL PAUL., The Florida State University
- Date Issued
- 1976, 1976
- Identifier
- AAI7616529, 2988236, FSDT2988236, fsu:72743
- Format
- Document (PDF)
- Title
- MECHANISMS OF DEVELOPMENTAL REGULATION OF AMINO ACID TRANSPORT IN NEUROSPORA CRASSA.
- Creator
-
TISDALE, JANET BOONE HERBERT., The Florida State University
- Date Issued
- 1972, 1972
- Identifier
- AAI7223016, 2986872, FSDT2986872, fsu:71381
- Format
- Document (PDF)
- Title
- ALLOSTERIC MODIFICATION OF THE NEUTRAL AMINO ACID TRANSPORT SYSTEM OF NEUROSPORA CRASSA.
- Creator
-
LARIMER, FRANK WILLIAM., The Florida State University
- Date Issued
- 1975, 1975
- Identifier
- AAI7613815, 2988192, FSDT2988192, fsu:72699
- Format
- Document (PDF)
- Title
- STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF POLYGALACTOSAMINE: A POSITIVELY CHARGED CELL WALL POLYSACCHARIDE OF NEUROSPORA CRASSA.
- Creator
-
JENSEN, JOHN WAYNE., The Florida State University
- Date Issued
- 1976, 1976
- Identifier
- AAI7713320, 2988497, FSDT2988497, fsu:73004
- Format
- Document (PDF)
- Title
- THE GENERAL AMINO ACID TRANSPORT SYSTEM OF NEUROSPORA CRASSA.
- Creator
-
MASON, RUTH., The Florida State University
- Date Issued
- 1978, 1978
- Identifier
- AAI7822188, 2988977, FSDT2988977, fsu:73484
- Format
- Document (PDF)
- Title
- PROGRAMMING OF DNA SYNTHESIS AND NATURE OF DNA SYNTHESIZED AT DIFFERENT TIMES IN THE S-PHASE OF THE CELL CYCLE OF A EUCARYOTIC SYSTEM.
- Creator
-
ADEGOKE, JOSEPH ADELEKE., The Florida State University
- Date Issued
- 1974, 1974
- Identifier
- AAI7504747, 2987720, FSDT2987720, fsu:72227
- Format
- Document (PDF)
- Title
- DEVELOPMENT OF CENTRIFUGATION METHODS AND THEIR APPLICATION IN NUCLEIC ACID RESEARCH.
- Creator
-
ANDREAN, BENVENUTO ANTONIO GUGLIELMO., The Florida State University
- Date Issued
- 1976, 1976
- Identifier
- AAI7628590, 2988287, FSDT2988287, fsu:72794
- Format
- Document (PDF)
- Title
- A STUDY OF ACETATE TRANSPORT AND ITS REGULATION IN NEUROSPORA CRASSA.
- Creator
-
RAO, KAMESWAR TELIKICHERLA., The Florida State University
- Date Issued
- 1976, 1976
- Identifier
- AAI7628634, 2988299, FSDT2988299, fsu:72806
- Format
- Document (PDF)
- Title
- PUTRESCINE TRANSPORT IN NORMAL AND CYSTIC FIBROSIS FIBROBLASTS.
- Creator
-
KELLY, JOANN CARYL., The Florida State University
- Date Issued
- 1976, 1976
- Identifier
- AAI7722122, 2988564, FSDT2988564, fsu:73071
- Format
- Document (PDF)
- Title
- RELEASE OF NASCENT FRAGMENTS FROM REPLICATING MAMMALIAN CELL DNA.
- Creator
-
GUY, ARTHUR LEE., The Florida State University
- Date Issued
- 1977, 1977
- Identifier
- AAI7804974, 2988816, FSDT2988816, fsu:73323
- Format
- Document (PDF)
- Title
- STUDIES OF TEMPERATURE-SENSITIVE OSMOTIC REMEDIAL MUTANTS OF NEUROSPORA CRASSA.
- Creator
-
MARTIN, CHARLES EVERETT., The Florida State University
- Date Issued
- 1972, 1972
- Identifier
- AAI7231414, 2986998, FSDT2986998, fsu:71507
- Format
- Document (PDF)
- Title
- PATTERNS OF GENETIC DIFFERENTIATION IN HIBISCUS SECT. TRIONUM.
- Creator
-
WISE, DWAYNE ALLISON., The Florida State University
- Date Issued
- 1972, 1972
- Identifier
- AAI7311328, 2987174, FSDT2987174, fsu:71683
- Format
- Document (PDF)
- Title
- THE RELATIONSHIP BETWEEN THE OBSERVED X-RAY-INDUCED CHROMOSOME ABERRATION FREQUENCY AND THE EVENTS OF MEIOSIS IN LILIUM LONGIFLORUM.
- Creator
-
SHULL, JULIAN KENNETH, JR., The Florida State University
- Date Issued
- 1973, 1973
- Identifier
- AAI7321355, 2987225, FSDT2987225, fsu:71734
- Format
- Document (PDF)
- Title
- ISOACCEPTING TRANSFER-RNA'S OF NEUROSPORA CRASSA: A DEVELOPMENTAL STUDY.
- Creator
-
JERVIS, HERBERT H., The Florida State University
- Date Issued
- 1973, 1973
- Identifier
- AAI7408398, 2987482, FSDT2987482, fsu:71991
- Format
- Document (PDF)
- Title
- REGULATION OF AMINO ACID TRANSPORT MEDIATED BY THE AMINO ACID POOL IN NEUROSPORA CRASSA.
- Creator
-
BRESCIA, VINCENT THOMAS., The Florida State University
- Date Issued
- 1973, 1973
- Identifier
- AAI7406588, 2987418, FSDT2987418, fsu:71927
- Format
- Document (PDF)
- Title
- A STUDY OF AMINO ACID TRANSPORT IN NEUROSPORA CRASSA.
- Creator
-
ROESS, WILLIAM BYRON., The Florida State University
- Date Issued
- 1966, 1966
- Identifier
- AAI6706484, 2985593, FSDT2985593, fsu:70102
- Format
- Document (PDF)
- Title
- THE HYDROGRAPHY AND PHYTOPLANKTON ECOLOGY OF THE INSHORE, NORTHEASTERN GULF OF MEXICO.
- Creator
-
CURL, HERBERT C., JR., The Florida State University
- Date Issued
- 1956, 1956
- Identifier
- AAI0018668, 2984757, FSDT2984757, fsu:69177
- Format
- Document (PDF)
- Title
- INHIBITION ANALYSIS OF AROMATIC AMINO ACID-REQUIRING MUTANTS OF NEUROSPORA CRASSA.
- Creator
-
BROCKMAN, HERMAN ELDON., The Florida State University
- Date Issued
- 1960, 1960
- Identifier
- AAI6003304, 2984922, FSDT2984922, fsu:69378
- Format
- Document (PDF)
- Title
- NITROUS ACID-INDUCED REVERSION AT THE AD-3 LOCUS OF NEUROSPORA CRASSA.
- Creator
-
BARNETT, WILLIAM EDGAR., The Florida State University
- Date Issued
- 1961, 1961
- Identifier
- AAI6101277, 2984937, FSDT2984937, fsu:69396
- Format
- Document (PDF)
- Title
- THE DEVELOPMENT OF A NEW BIOCHEMICAL GENETIC TOOL: PODOSPORA ANSERINA, NIESSL.
- Creator
-
PERHAM, JAMES EDWIN., The Florida State University
- Date Issued
- 1961, 1961
- Identifier
- AAI6103645, 2984973, FSDT2984973, fsu:69439
- Format
- Document (PDF)
- Title
- INHIBITION OF FERTILIZATION BY FUCUS EXTRACTS.
- Creator
-
BRANHAM, JOSEPH M., The Florida State University
- Date Issued
- 1963, 1963
- Identifier
- AAI6306347, 2985128, FSDT2985128, fsu:69628
- Format
- Document (PDF)
- Title
- CHARACTERIZATION OF A POLYRIBOSOMAL COMPLEX IN NEUROSPORA CRASSA.
- Creator
-
GRATZNER, HOWARD GEORGE., The Florida State University
- Date Issued
- 1964, 1964
- Identifier
- AAI6500330, 2985268, FSDT2985268, fsu:69779
- Format
- Document (PDF)
- Title
- CYTOCHROME B2 AND D-LACTATECYTOCHROME C REDUCTASE IN RESPIRATION DEFICIENT MUTANTS OF SACCHAROMYCES CEREVISIAE.
- Creator
-
ROSCH, EDWARD JAMISON., The Florida State University
- Date Issued
- 1962, 1962
- Identifier
- AAI6204629, 2985057, FSDT2985057, fsu:69542
- Format
- Document (PDF)
- Title
- MORPHOLOGICAL ASPECTS OF GAMETE CONTACT AND FUSION AND OF SPERM ENTRY INTO OOCYTES.
- Creator
-
FRANKLIN, LUTHER EDWARD., The Florida State University
- Date Issued
- 1963, 1963
- Identifier
- AAI6407579, 2985201, FSDT2985201, fsu:69712
- Format
- Document (PDF)
- Title
- CHEMICALLY INDUCED MOSAICISM IN DROSOPHILA.
- Creator
-
EPLER, JAMES LEON., The Florida State University
- Date Issued
- 1963, 1963
- Identifier
- AAI6407578, 2985200, FSDT2985200, fsu:69711
- Format
- Document (PDF)
- Title
- MUTATION SEGREGATION STUDIES IN NEUROSPORA CRASSA.
- Creator
-
BAYLIS, JOHN ROBERT, JR., The Florida State University
- Date Issued
- 1966, 1966
- Identifier
- AAI6700342, 2985546, FSDT2985546, fsu:70055
- Format
- Document (PDF)
- Title
- DNA REPAIR IN HAEMOPHILUS INFLUENZAE: ISOLATION AND CHARACTERIZATION OF AN ULTRAVIOLET SENSITIVE MUTATOR MUTANT (TRANSFORMATION, MISMATCH REPAIR, INACTIVATION).
- Creator
-
WALTER, RONALD BRUCE., Florida State University
- Abstract/Description
-
DNA repair in Haemophilus influenzae appears to be quite different from that seen in Escherichia coli in that H. influenzae shows neither "S.O.S." nor "adaptation" phenomena. Repair of DNA lesions in H. influenzae has been seen to occur via recombinational, excision, and mismatch repair pathways acting independently of one another. I have isolated an ultraviolet (UV)-sensitive mutator mutant (mutB1) of H. influenzae Rd which shows deficiencies in both recombinational and mismatch repair...
Show moreDNA repair in Haemophilus influenzae appears to be quite different from that seen in Escherichia coli in that H. influenzae shows neither "S.O.S." nor "adaptation" phenomena. Repair of DNA lesions in H. influenzae has been seen to occur via recombinational, excision, and mismatch repair pathways acting independently of one another. I have isolated an ultraviolet (UV)-sensitive mutator mutant (mutB1) of H. influenzae Rd which shows deficiencies in both recombinational and mismatch repair pathways. This mutant is sensitive to a variety of DNA damaging agents as well as being hypermutable by alkylating agents and base analogues. MutB1 cells do not show post-UV DNA breakdown but do begin excision after UV irradiation., Genetic transformation with UV-irradiated DNA on mutB1 recipients shows that high (HE) and low (LE) efficiency markers are transformed at a ratio of 1.0 as in the mismatch repair deficient hex1 mutant; however, kinetics of UV-inactivation experiments indicate that HE markers are sensitized and act as LE markers do on wild type recipients. Thus, the mutB gene product appears to play a role in both DNA repair and genetic transformation., An E. coli mutant (uvrD) having many similar properties to the mutB1 mutant has recently been shown deficient in DNA helicase II. A model is outlined which presents a role for a DNA helicase in both DNA repair and genetic transformation of H. influenzae.
Show less - Date Issued
- 1985, 1985
- Identifier
- AAI8522755, 3086197, FSDT3086197, fsu:75680
- Format
- Document (PDF)
- Title
- THE HEAT SHOCK RESPONSES OF NEUROSPORA CRASSA.
- Creator
-
LEE, KIL JAE., Florida State University
- Abstract/Description
-
All living organisms, when confronted with a number of different environmental changes, including heat shock, elicit a common and seemingly highly conserved response: the heat-shock response. The response is characterized by the rapid, preferential synthesis and accumulation of heat shock proteins (hsps). The multiplicity of responses of Neurospora crassa to a simple thermal stress has been analysed at several levels, molecular and cellular., The major changes in gene expression occur in...
Show moreAll living organisms, when confronted with a number of different environmental changes, including heat shock, elicit a common and seemingly highly conserved response: the heat-shock response. The response is characterized by the rapid, preferential synthesis and accumulation of heat shock proteins (hsps). The multiplicity of responses of Neurospora crassa to a simple thermal stress has been analysed at several levels, molecular and cellular., The major changes in gene expression occur in mycelia and conidia as their environmental temperature is increased from normal value (25(DEGREES)C) to those elevated temperature (36-48(DEGREES)C). At least eight hsps were greatly synthesized and simultaneously most of preexisting normal proteins were greatly reduced. Each hsp requires the different range of elevated temperatures and different lengths of time for initiation and maximal synthesis., The response of Neurospora to heat shock was transient during the continous heat-shock treatment: normal protein synthesis resumes and heat shock protein synthesis decreases., The heat-shock response was differentially expressed during the germination of conidia. Most of hsps were fully expressed only after 3 hours of pre-germination from dormant conidia., There is a good correlation between the induction of thermotolerance and increased synthesis of hsps. The transient pattern of induction, development, and decay of thermotolerance was very similar to the pattern of hsp synthesis at the same elevated temperature., The protocol for isolation of several hsps was developed using combination of two different chromatographic methods, including DEAE-HPLC and SE-HPLC., DNA sequences of Neurospora nuclear genome were cloned, which are highly expressed in heat-shocked cells.(' )
Show less - Date Issued
- 1987, 1987
- Identifier
- AAI8713324, 3086617, FSDT3086617, fsu:76092
- Format
- Document (PDF)
- Title
- MICROTUBULE ORGANIZATION, THE REGULATION OF MICROTUBULE ASSEMBLY AND DISASSEMBLY, AND THE FUNCTIONS OF THE MICROTUBULE-CONTAINING STRUCTURES IN SEA URCHIN EGGS DURING FERTILIZATION AND CELL DIVISION (MOTILITY).
- Creator
-
BALCZON, RONALD DAVID., Florida State University
- Abstract/Description
-
Anti-tubulin immunofluorescence microscopy was used to study the structures involved in the first cell cycle in the fertilized sea urchin egg and the regulation of microtubule assembly following fertilization., Four different microtubule-containing structures were observed in the egg cytoplasm at different times during the first cell cycle. The unfertilized egg was found to be devoid of microtubules. Immediately after fertilization, the sperm aster forms. This structure is transient, and is...
Show moreAnti-tubulin immunofluorescence microscopy was used to study the structures involved in the first cell cycle in the fertilized sea urchin egg and the regulation of microtubule assembly following fertilization., Four different microtubule-containing structures were observed in the egg cytoplasm at different times during the first cell cycle. The unfertilized egg was found to be devoid of microtubules. Immediately after fertilization, the sperm aster forms. This structure is transient, and is followed in sequence by the monaster, the streak, and the mitotic apparatus. The possible functions of each of these structures was investigated., The finding that the unfertilized egg does not contain any polymerized microtubules presented a perfect system to study the regulation of microtubule assembly in vivo. The formation of microtubules in vivo was studied in relation to DNA synthesis cycles, the presence or absence of centrioles, and the Ca('++)-fluxes and pH changes which accompany fertilization. It was determined that of these variables, the change in intracellular pH that accompanies fertilization is absolutely essential for microtubule assembly to commence in the egg cytoplasm., Investigations were carried out to determine the target or targets which were affected by the pH shift and were then subsequently responsible for making the egg cytoplasm conducive to supporting microtubule assembly. Ca('++)-sensitivity experiments were carried out on the sperm aster microtubules at various pH's and it was found that the microtubules themselves were not made more resistant to Ca('++) ions because of the pH change. From this it was concluded that the change in pH does not act directly on microtubules to support microtubule assembly. The cytoplasmic component that is the target of the pH shift remains to be determined.
Show less - Date Issued
- 1985, 1985
- Identifier
- AAI8513356, 3086087, FSDT3086087, fsu:75573
- Format
- Document (PDF)
- Title
- Ribosomal RNA operons in Streptococcus pneumoniae: Gene organization and copy number.
- Creator
-
Bacot, Christopher Matthew., Florida State University
- Abstract/Description
-
Ribosomes and transfer ribonucleic acids (tRNAs) are integral parts in the cells protein synthesis machinery. tRNAs are small polymers of ca. 77 RNA residues in length. The ribosome is a ribonucleo-protein, a large heterologous complex comprised of ca. 55 proteins and three species of ribosomal RNAs (rRNA). The ribosomal RNAs (and quite often the tRNAs) are organized on the chromosome in a close linear array known as the rRNA operons. The organization of these genes into multi-gene clusters...
Show moreRibosomes and transfer ribonucleic acids (tRNAs) are integral parts in the cells protein synthesis machinery. tRNAs are small polymers of ca. 77 RNA residues in length. The ribosome is a ribonucleo-protein, a large heterologous complex comprised of ca. 55 proteins and three species of ribosomal RNAs (rRNA). The ribosomal RNAs (and quite often the tRNAs) are organized on the chromosome in a close linear array known as the rRNA operons. The organization of these genes into multi-gene clusters ultimately facilitates the cell's regulation of the protein synthesis apparatus., Isoleucine and alanine tRNAs are encoded tandemly within the 16S-23S intergenic spacer of some eubacterial rRNA operons. A previous study has demonstrated that the intergenic spacer in the eubacterium S. pneumoniae is unconventional. The isoleucine tRNA is not tandemly encoded with the alanine tRNA. Rather it appears to be encoded at the distal end of the operon near the 5S rRNA gene. This unique organization, the presence of numerous direct repeats in the non-coding regions of the 16S-23S intergenic spacer, and the potential for stem-loop formation of the isoleucine tRNA gene suggest that it might have been rearranged intrachromosomally via an illegitimate recombination., Reported herein is a characterization of the distal spacer regions of the rRNA operons on the S. pneumoniae chromosome. Dot-blot hybridization analysis shows that the number of 16S rRNA genes on the S. pneumoniae chromosome is four. This is consistent with a previous study wherein the results suggested a minimum copy number of four for the rRNA operons. The distal spacer region from each of the four ribosomal RNA operons has been cloned and sequenced. An isoleucine tRNA is encoded on two of the four cloned fragments. DNA sequence analysis of the regions flanking the cloned isoleucine tRNA genes revealed motifs that are consistent with one of the popular models for illegitimate recombination.
Show less - Date Issued
- 1992, 1992
- Identifier
- AAI9309716, 3087998, FSDT3087998, fsu:76805
- Format
- Document (PDF)
- Title
- AN ULTRASTRUCTURAL EXAMINATION OF CHROMOSOME PAIRING DURING MEIOSIS (TRADESCANTIA).
- Creator
-
HASENKAMPF, CLARE A., Florida State University
- Abstract/Description
-
Whole mount spread preparations of Tradescantia nuclei were used to examine synaptonemal complex (SC) formation. Tradescantia spreads have the standard SC morphology. Kinetochores and recombination nodules were not regularly observed. Thickenings of the lateral elements were observed and increased in numbers between mid- and late-zygotene. The thickenings were distributed evenly amongst the chromosomes, but were nonrandomly distributed within a chromosome. They were underrepresented in...
Show moreWhole mount spread preparations of Tradescantia nuclei were used to examine synaptonemal complex (SC) formation. Tradescantia spreads have the standard SC morphology. Kinetochores and recombination nodules were not regularly observed. Thickenings of the lateral elements were observed and increased in numbers between mid- and late-zygotene. The thickenings were distributed evenly amongst the chromosomes, but were nonrandomly distributed within a chromosome. They were underrepresented in unsynapsed regions and overrepresented in synapsed regions, especially in synapsed regions near the junction with an unsynapsed region., Lateral component material is well formed along the chromosome axis before SC formation begins. In early- and mid-zygotene the telomeres occur in a restricted region and have modifications for attachment at or near the nuclear envelope. Initiation of SC occurred at many sites along the chromosomes; regions nearer the telomeres had a greater tendency to form SC first. Between 29 and 106 initiation sites were observed in the early-zygotene nuclei. The average distance between initiations ranged from 7.3 to 11.2 (mu)m. The total number of potential initiation sites was estimated and ranged from 250-299., Foldback synapsis was observed in all nuclei of Clone 02. The extent of the foldback synapsis was fairly constant at all stages examined., The results from Tradescantia and other organisms was considered, and a model of SC formation was proposed. The key aspect of the model is that SC formation does not require homology. Synthesis of zygotene DNA is required for SC formation and is regulated such that homologous regions are in close proximity as their zygotene DNA is synthesized. This proximity, just as homologous regions have been made competent to form SC, makes it highly likely that homologous SC formation will ensue.
Show less - Date Issued
- 1984, 1984
- Identifier
- AAI8408941, 3085781, FSDT3085781, fsu:75268
- Format
- Document (PDF)
