Current Search:  Viruses (x)

Search results

  • CSV Spreadsheet
(1 - 50 of 55)

Pages

Shortages Sign
Title
Shortages Sign.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
An image of a sign. It restricts how much meat a customer is allowed to buy.
Identifier
FSU_HUA_2020_016_020
Format
Image (JPEG)
The Many Faces of a Pandemic
Title
The Many Faces of a Pandemic.
Creator
Florida State University Libraries. Heritage & University Archives, De La Aguilera, Daniela
Abstract/Description
An essay examining how life is different due to the effects of COVID-19.
Identifier
FSU_HUA_2020_016_013
Format
Document (PDF)
Life in Quarantine
Title
Life in Quarantine.
Creator
Florida State University Libraries. Heritage & University Archives, Howard, Aleya
Abstract/Description
An essay that details the differences in life during the pandemic to life before the virus.
Identifier
FSU_HUA_2020_016_015
Format
Document (PDF)
Forest Fire:Burned by COVID-19
Title
Forest Fire:Burned by COVID-19.
Creator
Florida State University Libraries. Heritage & University Archives, Ballantyne, Aleycia
Abstract/Description
This essay compares the stages of ife with Covid-19 to the stages of a wildfire.
Identifier
FSU_HUA_2020_016_011
Format
Document (PDF)
Quarantine: Harmful or Helpful
Title
Quarantine: Harmful or Helpful.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
An essay about common problems/situations people find themselves in during the pandemic.
Identifier
FSU_HUA_2020_016_016
Format
Document (PDF)
Untitled
Title
Untitled.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
This text shares a story about a girl's life in isolation during quarantine
Identifier
FSU_HUA_2020_016_014
Format
Document (PDF)
Before the Virus
Title
Before the Virus.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
A student's report about how the world reacted to the pandemic
Identifier
FSU_HUA_2020_016_006
Format
Document (PDF)
At Home with the Collections Management Team
Title
At Home with the Collections Management Team.
Creator
Florida State University Libraries. Heritage & University Archives, Davis, Hannah
Abstract/Description
A multimedia essay detailing what remote work/life is like for student archival workers.
Identifier
FSU_HUA_2020_016_024
Format
Document (PDF)
Covid-19: Detailed Accounts
Title
Covid-19: Detailed Accounts.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
A multimedia diary of life during Covid-19
Identifier
FSU_HUA_2020_016_007
Format
Document (PDF)
COVID on Camera
Title
COVID on Camera.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
A multimedia essay of daily life in quarantine
Identifier
FSU_HUA_2020_016_012
Format
Document (PDF)
Directions
Title
Directions.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
An image of a "one way" sticker on the ground of an aisle at a Publix
Identifier
FSU_HUA_2020_016_022
Format
Image (JPEG)
Mask
Title
Mask.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
A photo of a homemade mask
Identifier
FSU_HUA_2020_016_005
Format
Image (JPEG)
Making Hand Sanitizer, 2020
Title
Making Hand Sanitizer, 2020.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
A collection of homemade hand sanitizer
Identifier
FSU_HUA_2020_016_002
Format
Image (JPEG)
Working From Home
Title
Working From Home.
Creator
Florida State University Libraries. Heritage & University Archives, Lee, Shaundra
Abstract/Description
A photo of a laptop computer and a teddy bear.
Identifier
FSU_HUA_2020_016_019
Format
Image (JPEG)
Fountains
Title
Fountains.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
An image of out of service water fountains at a Publix.
Identifier
FSU_HUA_2020_016_021
Format
Image (JPEG)
Remote Work
Title
Remote Work.
Creator
Florida State University Libraries. Heritage & University Archives, Maki, David
Abstract/Description
A photo of computer screens used for remote work.
Identifier
FSU_HUA_2020_016_017
Format
Image (JPEG)
Working From Home
Title
Working From Home.
Creator
Florida State University Libraries. Heritage & University Archives, Lee, Shaundra
Abstract/Description
A photo of a woman in quarantine.
Identifier
FSU_HUA_2020_016_018
Format
Image (JPEG)
Life with COVID-19
Title
Life with COVID-19.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
An essay written to document a person's life during the pandemic.
Identifier
FSU_HUA_2020_016_008
Format
Document (PDF)
Untitled
Title
Untitled.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
A personal essay documenting the contrasts between life before and after quarantine
Identifier
FSU_HUA_2020_016_009
Format
Document (PDF)
Sweet Corona
Title
Sweet Corona.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
A poem about the COVID-19 virus
Identifier
FSU_HUA_2020_016_010
Format
Document (PDF)
Park Closure Sign
Title
Park Closure Sign.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
A swing set blocked off with a sign that reads "This Area is Closed".
Identifier
FSU_HUA_2020_016_001
Format
Image (JPEG)
Empty Shelves
Title
Empty Shelves.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
An image of empty cleaning product shelves as a store.
Identifier
FSU_HUA_2020_016_023
Format
Image (JPEG)
Masks Sign
Title
Masks Sign.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
A sign describing a market's plan for coronavirus protection.
Identifier
FSU_HUA_2020_016_004
Format
Image (JPEG)
Masks Sign
Title
Masks Sign.
Creator
Florida State University Libraries. Heritage & University Archives, Mon, Lorraine
Abstract/Description
A sign describing a market's plan for coronavirus protection.
Identifier
FSU_HUA_2020_016_003
Format
Image (JPEG)
President T.K. Wetherell statue abiding by FSU's mask policy during the COVID-19 pandemic
Title
President T.K. Wetherell statue abiding by FSU's mask policy during the COVID-19 pandemic.
Creator
Florida State University Libraries. Heritage & University Archives, Aime, Kari Marie
Abstract/Description
An image of President Wetherell's statue on Westcott Plaza wearing a mask.
Identifier
FSU_HUA_2020_016_025
Format
Image (JPEG)
Social Distancing in Line at Subway on FSU main campus
Title
Social Distancing in Line at Subway on FSU main campus.
Creator
Florida State University Libraries. Heritage & University Archives, Marquez, Maria Ricah
Abstract/Description
An image of customers social distancing in line at the Subway restaurant located in the Parking Garage at Woodward on FSU's main campus.
Identifier
FSU_HUA_2020_016_026
Format
Image (JPEG)
6 feet apart floor decals at Subway on FSU main campus
Title
6 feet apart floor decals at Subway on FSU main campus.
Creator
Florida State University Libraries. Heritage & University Archives, Marquez, Maria Ricah
Abstract/Description
An image of social distancing line decals and removed customer seating at the Subway restaurant located in the Parking Garage at Woodward on FSU's main campus.
Identifier
FSU_HUA_2020_016_027
Format
Image (JPEG)
Family Photos
Title
Family Photos.
Creator
Florida State University Libraries. Heritage & University Archives, Loustau, Trystan Hanna
Abstract/Description
This student shares their experiences during the coronavirus pandemic as they came home to care for family while their mother was deployed as a Navy Reserves Nurse in New York City. They included several photographs taken by their mother in New York City as well as a photogrpah of them with their mother.
Identifier
FSU_HUA_2020_016_030_parent
Format
Set of related objects
Blog post on quarantining with family
Title
Blog post on quarantining with family.
Creator
Florida State University Libraries. Heritage & University Archives
Abstract/Description
A student's blog post about their reactions to being quarantined at home with their family as an introvert.
Identifier
FSU_HUA_2020_016_034
Format
Document (PDF)
My experiences with Covid-19
Title
My experiences with Covid-19.
Creator
Florida State University Libraries. Heritage & University Archives, Khan, Dean
Abstract/Description
This student shares their experiences during the coronavirus pandemic.
Identifier
FSU_HUA_2020_016_029
Format
Document (PDF)
Finding Hope
Title
Finding Hope.
Creator
Florida State University Libraries. Heritage & University Archives, Steidley, Elizabeth Paige
Abstract/Description
This student shares how they have used the lens of their mother's breast cancer diagnosis to understand behaviors during the coronavirus pandemic.
Identifier
FSU_HUA_2020_016_035
Format
Document (PDF)
Westcott Fountain behind fencing
Title
Westcott Fountain behind fencing.
Creator
Florida State University Libraries. Heritage & University Archives, Bertram, Robyn
Abstract/Description
An image of Westcott Fountain with a chainlink fence around it.
Identifier
FSU_HUA_2020_016_028
Format
Image (JPEG)
DNAase I hypersensitive site and their correlation to the differential expression of exogenous thymidine kinase gene
Title
DNAase I hypersensitive site and their correlation to the differential expression of exogenous thymidine kinase gene.
Creator
Lopez, Jose Victor
Date Issued
1988
Identifier
18344323, FSU_QP609D4L661988
Format
Document (PDF)
Advances in Zika Virus Research
Title
Advances in Zika Virus Research: Stem Cell Models, Challenges, and Opportunities..
Creator
Ming, Guo-Li, Tang, Hengli, Song, Hongjun
Abstract/Description
The re-emergence of Zika virus (ZIKV) and its suspected link with various disorders in newborns and adults led the World Health Organization to declare a global health emergency. In response, the stem cell field quickly established platforms for modeling ZIKV exposure using human pluripotent stem cell-derived neural progenitors and brain organoids, fetal tissues, and animal models. These efforts provided significant insight into cellular targets, pathogenesis, and underlying biological...
Show moreThe re-emergence of Zika virus (ZIKV) and its suspected link with various disorders in newborns and adults led the World Health Organization to declare a global health emergency. In response, the stem cell field quickly established platforms for modeling ZIKV exposure using human pluripotent stem cell-derived neural progenitors and brain organoids, fetal tissues, and animal models. These efforts provided significant insight into cellular targets, pathogenesis, and underlying biological mechanisms of ZIKV infection as well as platforms for drug testing. Here we review the remarkable progress in stem cell-based ZIKV research and discuss current challenges and future opportunities.
Show less
Date Issued
2016-12-01
Identifier
FSU_pmch_27912090, 10.1016/j.stem.2016.11.014, PMC5218815, 27912090, 27912090, S1934-5909(16)30415-5
Format
Citation
Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth.
Title
Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth.
Creator
Tang, Hengli, Hammack, Christy, Ogden, Sarah C, Wen, Zhexing, Qian, Xuyu, Li, Yujing, Yao, Bing, Shin, Jaehoon, Zhang, Feiran, Lee, Emily M, Christian, Kimberly M, Didier, Ruth...
Show moreTang, Hengli, Hammack, Christy, Ogden, Sarah C, Wen, Zhexing, Qian, Xuyu, Li, Yujing, Yao, Bing, Shin, Jaehoon, Zhang, Feiran, Lee, Emily M, Christian, Kimberly M, Didier, Ruth A, Jin, Peng, Song, Hongjun, Ming, Guo-Li
Show less
Abstract/Description
The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficiently infects human neural progenitor cells (hNPCs) derived from induced pluripotent stem cells. Infected hNPCs further release infectious ZIKV particles. Importantly, ZIKV infection...
Show moreThe suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficiently infects human neural progenitor cells (hNPCs) derived from induced pluripotent stem cells. Infected hNPCs further release infectious ZIKV particles. Importantly, ZIKV infection increases cell death and dysregulates cell-cycle progression, resulting in attenuated hNPC growth. Global gene expression analysis of infected hNPCs reveals transcriptional dysregulation, notably of cell-cycle-related pathways. Our results identify hNPCs as a direct ZIKV target. In addition, we establish a tractable experimental model system to investigate the impact and mechanism of ZIKV on human brain development and provide a platform to screen therapeutic compounds.
Show less
Date Issued
2016-05-05
Identifier
FSU_pmch_26952870, 10.1016/j.stem.2016.02.016, PMC5299540, 26952870, 26952870, S1934-5909(16)00106-5
Format
Citation
Medical Concerns in HIV-Positive Aging
Title
Medical Concerns in HIV-Positive Aging.
Creator
Simone, Mark, Appelbaum, Jonathan
Abstract/Description
The management and prognosis of HIV disease has changed dramatically since the introduction of combination HIV antiretroviral therapy in 1996. Thus, the number of older adults with HIV is increasing partly because people with HIV are living longer. At the same time, the rates of new HIV cases in older adults (usually defined as people over the age of 50) are also increasing. Currently, about 25 percent of all patients living with HIV are older than 50, and by 2015 adults older than 50 will...
Show moreThe management and prognosis of HIV disease has changed dramatically since the introduction of combination HIV antiretroviral therapy in 1996. Thus, the number of older adults with HIV is increasing partly because people with HIV are living longer. At the same time, the rates of new HIV cases in older adults (usually defined as people over the age of 50) are also increasing. Currently, about 25 percent of all patients living with HIV are older than 50, and by 2015 adults older than 50 will account for 50 percent of the population living with HIV.1 These trends make understanding the medical challenges of HIV in older adults more important than ever. This article reviews the special issues associated with HIV and AIDS in an older population.
Show less
Date Issued
2009
Identifier
FSU_migr_clinicalsciences_faculty_publications-0001
Format
Citation
Correlates of HPV Knowledge in the Era of HPV Vaccination
Title
Correlates of HPV Knowledge in the Era of HPV Vaccination: A Study of Unvaccinated Young Adult Women.
Creator
Gerend, Mary, Shepherd, Janet
Abstract/Description
Until recently, awareness of the sexually transmitted infection human papillomavirus (HPV)—the virus that causes cervical cancer—was relatively low. The purpose of this study was to identify factors associated with HPV knowledge now that HPV vaccines have become widely available. Young adult women (n = 739; aged 18-26 years) attending Florida State University who had not yet initiated HPV vaccination completed a survey between March-August 2009. The survey assessed HPV awareness, HPV...
Show moreUntil recently, awareness of the sexually transmitted infection human papillomavirus (HPV)—the virus that causes cervical cancer—was relatively low. The purpose of this study was to identify factors associated with HPV knowledge now that HPV vaccines have become widely available. Young adult women (n = 739; aged 18-26 years) attending Florida State University who had not yet initiated HPV vaccination completed a survey between March-August 2009. The survey assessed HPV awareness, HPV knowledge, demographics, socio-political variables, sexual history, and health history variables. Over 97% of participants were aware of HPV prior to study enrollment; however, knowledge of HPV was only moderate. A multivariate regression analysis examining factors related to HPV knowledge revealed five independent correlates: Latina ethnicity, premarital sex values, number of lifetime sexual partners, history of cervical dysplasia, and HIV testing. These variables accounted for 14% of the variance in HPV knowledge. Less knowledge was observed for Latinas and women opposed to premarital sex. Greater knowledge was observed for women who had been tested for HIV and women with more sexual partners or a history of cervical dysplasia. These findings can inform future HPV vaccination campaigns and may be particularly useful in developing interventions for individuals with the largest deficits in HPV knowledge.
Show less
Date Issued
2011
Identifier
FSU_migr_mhs-0008, 10.1080/03630242.2011.540744
Format
Citation
What is the Most Effective Treatment for Bell's Palsy Among Patients with Diabetes?
Title
What is the Most Effective Treatment for Bell's Palsy Among Patients with Diabetes?.
Creator
Gonzalez, David, Rodríguez, Jose, Talbot, Richard
Abstract/Description
Patients with Bell's palsy have higher cure rates when treated with oral prednisolone within 72 hours of onset (SOR B, based on a single randomized controlled trial), but it is unclear what effect this therapy would have on patients with diabetes. Intravenous lipo-prostaglandin E1 (lipo-PGE1) may be an alternative to steroid therapy for Bell's palsy that does not effect blood glucose levels. (SOR C, based on a single small cohort study.)
Date Issued
2010
Identifier
FSU_migr_fmr-0024
Format
Citation
Molecular signatures associated with ZIKV exposure in human cortical neural progenitors.
Title
Molecular signatures associated with ZIKV exposure in human cortical neural progenitors.
Creator
Zhang, Feiran, Hammack, Christy, Ogden, Sarah C, Cheng, Yichen, Lee, Emily M, Wen, Zhexing, Qian, Xuyu, Nguyen, Ha Nam, Li, Yujing, Yao, Bing, Xu, Miao, Xu, Tianlei, Chen, Li,...
Show moreZhang, Feiran, Hammack, Christy, Ogden, Sarah C, Cheng, Yichen, Lee, Emily M, Wen, Zhexing, Qian, Xuyu, Nguyen, Ha Nam, Li, Yujing, Yao, Bing, Xu, Miao, Xu, Tianlei, Chen, Li, Wang, Zhiqin, Feng, Hao, Huang, Wei-Kai, Yoon, Ki-Jun, Shan, Chao, Huang, Luoxiu, Qin, Zhaohui, Christian, Kimberly M, Shi, Pei-Yong, Xu, Mingjiang, Xia, Menghang, Zheng, Wei, Wu, Hao, Song, Hongjun, Tang, Hengli, Ming, Guo-Li, Jin, Peng
Show less
Abstract/Description
Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs brain development and function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed to Asian ZIKV, African ZIKV, and dengue virus (DENV). In contrast to the robust global transcriptome changes induced by DENV, ZIKV has a more selective and larger impact on expression of genes involved in DNA replication and repair. While overall...
Show moreZika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs brain development and function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed to Asian ZIKV, African ZIKV, and dengue virus (DENV). In contrast to the robust global transcriptome changes induced by DENV, ZIKV has a more selective and larger impact on expression of genes involved in DNA replication and repair. While overall expression profiles are similar, ZIKV, but not ZIKV, induces upregulation of viral response genes and TP53. P53 inhibitors can block the apoptosis induced by both ZIKV and ZIKV in hNPCs, with higher potency against ZIKV-induced apoptosis. Our analyses reveal virus- and strain-specific molecular signatures associated with ZIKV infection. These datasets will help to investigate ZIKV-host interactions and identify neurovirulence determinants of ZIKV.
Show less
Date Issued
2016-10-14
Identifier
FSU_pmch_27580721, 10.1093/nar/gkw765, PMC5063002, 27580721, 27580721, gkw765
Format
Citation
Exosomal communication goes viral.
Title
Exosomal communication goes viral.
Creator
Meckes, David G
Abstract/Description
Exosomes are small vesicles secreted from cells that participate in intercellular communication events. Accumulating evidence demonstrates that host exosome pathways are hijacked by viruses and that virally modified exosomes contribute to virus spread and immune evasion. In the case of tumor viruses, recent findings suggest that alterations in normal exosome biology may promote the development and progression of cancer. These studies will be discussed in the context of our current knowledge...
Show moreExosomes are small vesicles secreted from cells that participate in intercellular communication events. Accumulating evidence demonstrates that host exosome pathways are hijacked by viruses and that virally modified exosomes contribute to virus spread and immune evasion. In the case of tumor viruses, recent findings suggest that alterations in normal exosome biology may promote the development and progression of cancer. These studies will be discussed in the context of our current knowledge of Epstein-Barr virus (EBV)-modified exosomes.
Show less
Date Issued
2015-05-01
Identifier
FSU_pmch_25740980, 10.1128/JVI.02470-14, PMC4442506, 25740980, 25740980, JVI.02470-14
Format
Citation
Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure.
Title
Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure.
Creator
Qian, Xuyu, Nguyen, Ha Nam, Song, Mingxi M, Hadiono, Christopher, Ogden, Sarah C, Hammack, Christy, Yao, Bing, Hamersky, Gregory R, Jacob, Fadi, Zhong, Chun, Yoon, Ki-Jun, Jeang...
Show moreQian, Xuyu, Nguyen, Ha Nam, Song, Mingxi M, Hadiono, Christopher, Ogden, Sarah C, Hammack, Christy, Yao, Bing, Hamersky, Gregory R, Jacob, Fadi, Zhong, Chun, Yoon, Ki-Jun, Jeang, William, Lin, Li, Li, Yujing, Thakor, Jai, Berg, Daniel A, Zhang, Ce, Kang, Eunchai, Chickering, Michael, Nauen, David, Ho, Cheng-Ying, Wen, Zhexing, Christian, Kimberly M, Shi, Pei-Yong, Maher, Brady J, Wu, Hao, Jin, Peng, Tang, Hengli, Song, Hongjun, Ming, Guo-Li
Show less
Abstract/Description
Cerebral organoids, three-dimensional cultures that model organogenesis, provide a new platform to investigate human brain development. High cost, variability, and tissue heterogeneity limit their broad applications. Here, we developed a miniaturized spinning bioreactor (SpinΩ) to generate forebrain-specific organoids from human iPSCs. These organoids recapitulate key features of human cortical development, including progenitor zone organization, neurogenesis, gene expression, and, notably, a...
Show moreCerebral organoids, three-dimensional cultures that model organogenesis, provide a new platform to investigate human brain development. High cost, variability, and tissue heterogeneity limit their broad applications. Here, we developed a miniaturized spinning bioreactor (SpinΩ) to generate forebrain-specific organoids from human iPSCs. These organoids recapitulate key features of human cortical development, including progenitor zone organization, neurogenesis, gene expression, and, notably, a distinct human-specific outer radial glia cell layer. We also developed protocols for midbrain and hypothalamic organoids. Finally, we employed the forebrain organoid platform to model Zika virus (ZIKV) exposure. Quantitative analyses revealed preferential, productive infection of neural progenitors with either African or Asian ZIKV strains. ZIKV infection leads to increased cell death and reduced proliferation, resulting in decreased neuronal cell-layer volume resembling microcephaly. Together, our brain-region-specific organoids and SpinΩ provide an accessible and versatile platform for modeling human brain development and disease and for compound testing, including potential ZIKV antiviral drugs.
Show less
Date Issued
2016-05-19
Identifier
FSU_pmch_27118425, 10.1016/j.cell.2016.04.032, PMC4900885, 27118425, 27118425, S0092-8674(16)30467-6
Format
Citation
Viremia and Clinical Presentation in Nicaraguan Patients Infected With Zika Virus, Chikungunya Virus, and Dengue Virus.
Title
Viremia and Clinical Presentation in Nicaraguan Patients Infected With Zika Virus, Chikungunya Virus, and Dengue Virus.
Creator
Waggoner, Jesse J, Gresh, Lionel, Vargas, Maria Jose, Ballesteros, Gabriela, Tellez, Yolanda, Soda, K James, Sahoo, Malaya K, Nuñez, Andrea, Balmaseda, Angel, Harris, Eva,...
Show moreWaggoner, Jesse J, Gresh, Lionel, Vargas, Maria Jose, Ballesteros, Gabriela, Tellez, Yolanda, Soda, K James, Sahoo, Malaya K, Nuñez, Andrea, Balmaseda, Angel, Harris, Eva, Pinsky, Benjamin A
Show less
Abstract/Description
 Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV) cocirculate in Nicaragua. In this study, we sought to compare the quantified viremia and clinical presentation of patients infected with 1 or more of these viruses.  Acute-phase serum samples from 346 patients with a suspected arboviral illness were tested using a multiplex real-time reverse-transcription polymerase chain reaction for ZIKV, CHIKV, and DENV. Viremia was quantitated for each detected virus, and clinical...
Show more Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV) cocirculate in Nicaragua. In this study, we sought to compare the quantified viremia and clinical presentation of patients infected with 1 or more of these viruses.  Acute-phase serum samples from 346 patients with a suspected arboviral illness were tested using a multiplex real-time reverse-transcription polymerase chain reaction for ZIKV, CHIKV, and DENV. Viremia was quantitated for each detected virus, and clinical information from request forms submitted with each sample was recorded.  A total of 263 patients tested positive for 1 or more viruses: 192 patients tested positive for a single virus (monoinfections) and 71 patients tested positive for 2 or all 3 viruses (coinfections). Quantifiable viremia was lower in ZIKV infections compared with CHIKV or DENV (mean 4.70 vs 6.42 and 5.84 log copies/mL serum, respectively; P < .001 for both comparisons), and for each virus, mean viremia was significantly lower in coinfections than in monoinfections. Compared with patients with CHIKV or DENV, ZIKV patients were more likely to have a rash (P < .001) and less likely to be febrile (P < .05) or require hospitalization (P < .001). Among all patients, hospitalized cases had higher viremia than those who did not require hospitalization (7.1 vs 4.1 log10 copies/mL serum, respectively; P < .001).  ZIKV, CHIKV, and DENV result in similar clinical presentations, and coinfections may be relatively common. Our findings illustrate the need for accurate, multiplex diagnostics for patient care and epidemiologic surveillance.
Show less
Date Issued
2016-12-15
Identifier
FSU_pmch_27578819, 10.1093/cid/ciw589, PMC5146717, 27578819, 27578819, ciw589
Format
Citation
Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen.
Title
Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen.
Creator
Xu, Miao, Lee, Emily M, Wen, Zhexing, Cheng, Yichen, Huang, Wei-Kai, Qian, Xuyu, Tcw, Julia, Kouznetsova, Jennifer, Ogden, Sarah C, Hammack, Christy, Jacob, Fadi, Nguyen, Ha Nam...
Show moreXu, Miao, Lee, Emily M, Wen, Zhexing, Cheng, Yichen, Huang, Wei-Kai, Qian, Xuyu, Tcw, Julia, Kouznetsova, Jennifer, Ogden, Sarah C, Hammack, Christy, Jacob, Fadi, Nguyen, Ha Nam, Itkin, Misha, Hanna, Catherine, Shinn, Paul, Allen, Chase, Michael, Samuel G, Simeonov, Anton, Huang, Wenwei, Christian, Kimberly M, Goate, Alison, Brennand, Kristen J, Huang, Ruili, Xia, Menghang, Ming, Guo-Li, Zheng, Wei, Song, Hongjun, Tang, Hengli
Show less
Abstract/Description
In response to the current global health emergency posed by the Zika virus (ZIKV) outbreak and its link to microcephaly and other neurological conditions, we performed a drug repurposing screen of ∼6,000 compounds that included approved drugs, clinical trial drug candidates and pharmacologically active compounds; we identified compounds that either inhibit ZIKV infection or suppress infection-induced caspase-3 activity in different neural cells. A pan-caspase inhibitor, emricasan, inhibited...
Show moreIn response to the current global health emergency posed by the Zika virus (ZIKV) outbreak and its link to microcephaly and other neurological conditions, we performed a drug repurposing screen of ∼6,000 compounds that included approved drugs, clinical trial drug candidates and pharmacologically active compounds; we identified compounds that either inhibit ZIKV infection or suppress infection-induced caspase-3 activity in different neural cells. A pan-caspase inhibitor, emricasan, inhibited ZIKV-induced increases in caspase-3 activity and protected human cortical neural progenitors in both monolayer and three-dimensional organoid cultures. Ten structurally unrelated inhibitors of cyclin-dependent kinases inhibited ZIKV replication. Niclosamide, a category B anthelmintic drug approved by the US Food and Drug Administration, also inhibited ZIKV replication. Finally, combination treatments using one compound from each category (neuroprotective and antiviral) further increased protection of human neural progenitors and astrocytes from ZIKV-induced cell death. Our results demonstrate the efficacy of this screening strategy and identify lead compounds for anti-ZIKV drug development.
Show less
Date Issued
2016-10-01
Identifier
FSU_pmch_27571349, 10.1038/nm.4184, PMC5386783, 27571349, 27571349, nm.4184
Format
Citation
ORF33 and ORF38 of Kaposi's Sarcoma-Associated Herpesvirus Interact and Are Required for Optimal Production of Infectious Progeny Viruses.
Title
ORF33 and ORF38 of Kaposi's Sarcoma-Associated Herpesvirus Interact and Are Required for Optimal Production of Infectious Progeny Viruses.
Creator
Wu, Jian-Jun, Avey, Denis, Li, Wenwei, Gillen, Joseph, Fu, Bishi, Miley, Wendell, Whitby, Denise, Zhu, Fanxiu
Abstract/Description
We recently showed that the interaction between Kaposi's sarcoma-associated herpesvirus (KSHV) tegument proteins ORF33 and ORF45 is crucial for progeny virion production, but the exact functions of KSHV ORF33 during lytic replication were unknown (J. Gillen, W. Li, Q. Liang, D. Avey, J. Wu, F. Wu, J. Myoung, and F. Zhu, J Virol 89:4918-4931, 2015, http://dx.doi.org/10.1128/JVI.02925-14). Therefore, here we investigated the relationship between ORF33 and ORF38, whose counterparts in both alpha...
Show moreWe recently showed that the interaction between Kaposi's sarcoma-associated herpesvirus (KSHV) tegument proteins ORF33 and ORF45 is crucial for progeny virion production, but the exact functions of KSHV ORF33 during lytic replication were unknown (J. Gillen, W. Li, Q. Liang, D. Avey, J. Wu, F. Wu, J. Myoung, and F. Zhu, J Virol 89:4918-4931, 2015, http://dx.doi.org/10.1128/JVI.02925-14). Therefore, here we investigated the relationship between ORF33 and ORF38, whose counterparts in both alpha- and betaherpesviruses interact with each other. Using specific monoclonal antibodies, we found that both proteins are expressed during the late lytic cycle with similar kinetics and that both are present in mature virions as components of the tegument. Furthermore, we confirmed that ORF33 interacts with ORF38. Interestingly, we observed that ORF33 tightly associates with the capsid, whereas ORF38 associates with the envelope. We generated ORF33-null, ORF38-null, and double-null mutants and found that these mutants apparently have identical phenotypes: the mutations caused no apparent effect on viral gene expression but reduced the yield of progeny virion by about 10-fold. The progeny virions also lack certain virion component proteins, including ORF45. During viral lytic replication, the virions associate with cytoplasmic vesicles. We also observed that ORF38 associates with the membranes of vesicles and colocalizes with the Golgi membrane or early endosome membrane. Further analyses of ORF33/ORF38 mutants revealed the reduced production of virion-containing vesicles, suggesting that ORF33 and ORF38 are involved in the transport of newly assembled viral particles into cytoplasmic vesicles, a process important for viral maturation and egress. Herpesvirus assembly is an essential step in virus propagation that leads to the generation of progeny virions. It is a complicated process that depends on the delicate regulation of interactions among virion proteins. We previously revealed an essential role of ORF45-ORF33 binding for virus assembly. Here, we report that ORF33 and its binding partner, ORF38, are required for infectious virus production due to their important role in the tegumentation process. Moreover, we found that both ORF33 and ORF38 are involved in the transportation of virions through vesicles during maturation and egress. Our results provide new insights into the important roles of ORF33 and ORF38 during viral assembly, a process critical for virus propagation that is intimately linked to KSHV pathobiology.
Show less
Date Issued
2015-12-04
Identifier
FSU_pmch_26637455, 10.1128/JVI.02738-15, PMC4734004, 26637455, 26637455, JVI.02738-15
Format
Citation
Beyond Structural Biology to Functional Biology
Title
Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance..
Creator
Qin, Huajun, Miao, Yimin, Cross, Timothy A, Fu, Riqiang
Abstract/Description
In terms of structural biology, solid-state NMR experiments and strategies have been well established for resonance assignments, leading to the determination of three-dimensional structures of insoluble membrane proteins in their native-like environment. It is also known that NMR has the unique capabilities to characterize structure-function relationships of membrane-bound biological systems beyond structural biology. Here, we report on solid-state NMR experiments and strategies for...
Show moreIn terms of structural biology, solid-state NMR experiments and strategies have been well established for resonance assignments, leading to the determination of three-dimensional structures of insoluble membrane proteins in their native-like environment. It is also known that NMR has the unique capabilities to characterize structure-function relationships of membrane-bound biological systems beyond structural biology. Here, we report on solid-state NMR experiments and strategies for extracting functional activities on a sub-millisecond time scale. Specifically, we use the His37-labeled full length M2 (M2FL) protein of the Influenza A virus embedded in synthetic lipid bilayers as an example to characterize the proton conduction mechanism and kinetics. The integral membrane M2 protein assembles as a tetrameric bundle to form a proton-conducting channel that is activated by low pH and is essential for the viral lifecycle. Our results present convincing evidence for the formation of imidazolium-imidazole hydrogen bonds in the His37 tetrad at low pH and that these hydrogen bonds have a low barrier that facilitates the proton conduction mechanism in the M2FL protein. Moreover, it has been possible to measure hydronium ion exchange between water and the protons in the His37 NH bonds based on chemical exchange spectroscopy with minimized spin diffusion. The results identify an exchange rate constant of ∼4000 s for pH 5.8 at -10 °C.
Show less
Date Issued
2017-05-11
Identifier
FSU_pmch_28425709, 10.1021/acs.jpcb.7b02468, PMC5842430, 28425709, 28425709
Format
Citation
Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins.
Title
Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins.
Creator
Yoon, Ki-Jun, Song, Guang, Qian, Xuyu, Pan, Jianbo, Xu, Dan, Rho, Hee-Sool, Kim, Nam-Shik, Habela, Christa, Zheng, Lily, Jacob, Fadi, Zhang, Feiran, Lee, Emily M, Huang, Wei-Kai...
Show moreYoon, Ki-Jun, Song, Guang, Qian, Xuyu, Pan, Jianbo, Xu, Dan, Rho, Hee-Sool, Kim, Nam-Shik, Habela, Christa, Zheng, Lily, Jacob, Fadi, Zhang, Feiran, Lee, Emily M, Huang, Wei-Kai, Ringeling, Francisca Rojas, Vissers, Caroline, Li, Cui, Yuan, Ling, Kang, Koeun, Kim, Sunghan, Yeo, Junghoon, Cheng, Yichen, Liu, Sheng, Wen, Zhexing, Qin, Cheng-Feng, Wu, Qingfeng, Christian, Kimberly M, Tang, Hengli, Jin, Peng, Xu, Zhiheng, Qian, Jiang, Zhu, Heng, Song, Hongjun, Ming, Guo-Li
Show less
Abstract/Description
Zika virus (ZIKV) directly infects neural progenitors and impairs their proliferation. How ZIKV interacts with the host molecular machinery to impact neurogenesis in vivo is not well understood. Here, by systematically introducing individual proteins encoded by ZIKV into the embryonic mouse cortex, we show that expression of ZIKV-NS2A, but not Dengue virus (DENV)-NS2A, leads to reduced proliferation and premature differentiation of radial glial cells and aberrant positioning of newborn...
Show moreZika virus (ZIKV) directly infects neural progenitors and impairs their proliferation. How ZIKV interacts with the host molecular machinery to impact neurogenesis in vivo is not well understood. Here, by systematically introducing individual proteins encoded by ZIKV into the embryonic mouse cortex, we show that expression of ZIKV-NS2A, but not Dengue virus (DENV)-NS2A, leads to reduced proliferation and premature differentiation of radial glial cells and aberrant positioning of newborn neurons. Mechanistically, in vitro mapping of protein-interactomes and biochemical analysis suggest interactions between ZIKA-NS2A and multiple adherens junction complex (AJ) components. Functionally, ZIKV-NS2A, but not DENV-NS2A, destabilizes the AJ complex, resulting in impaired AJ formation and aberrant radial glial fiber scaffolding in the embryonic mouse cortex. Similarly, ZIKA-NS2A, but not DENV-NS2A, reduces radial glial cell proliferation and causes AJ deficits in human forebrain organoids. Together, our results reveal pathogenic mechanisms underlying ZIKV infection in the developing mammalian brain.
Show less
Date Issued
2017-09-07
Identifier
FSU_pmch_28826723, 10.1016/j.stem.2017.07.014, PMC5600197, 28826723, 28826723, S1934-5909(17)30293-X
Format
Citation
Zika virus directly infects peripheral neurons and induces cell death.
Title
Zika virus directly infects peripheral neurons and induces cell death.
Creator
Oh, Yohan, Zhang, Feiran, Wang, Yaqing, Lee, Emily M, Choi, In Young, Lim, Hotae, Mirakhori, Fahimeh, Li, Ronghua, Huang, Luoxiu, Xu, Tianlei, Wu, Hao, Li, Cui, Qin, Cheng-Feng,...
Show moreOh, Yohan, Zhang, Feiran, Wang, Yaqing, Lee, Emily M, Choi, In Young, Lim, Hotae, Mirakhori, Fahimeh, Li, Ronghua, Huang, Luoxiu, Xu, Tianlei, Wu, Hao, Li, Cui, Qin, Cheng-Feng, Wen, Zhexing, Wu, Qing-Feng, Tang, Hengli, Xu, Zhiheng, Jin, Peng, Song, Hongjun, Ming, Guo-Li, Lee, Gabsang
Show less
Abstract/Description
Zika virus (ZIKV) infection is associated with neurological disorders of both the CNS and peripheral nervous systems (PNS), yet few studies have directly examined PNS infection. Here we show that intraperitoneally or intraventricularly injected ZIKV in the mouse can infect and impact peripheral neurons in vivo. Moreover, ZIKV productively infects stem-cell-derived human neural crest cells and peripheral neurons in vitro, leading to increased cell death, transcriptional dysregulation and cell...
Show moreZika virus (ZIKV) infection is associated with neurological disorders of both the CNS and peripheral nervous systems (PNS), yet few studies have directly examined PNS infection. Here we show that intraperitoneally or intraventricularly injected ZIKV in the mouse can infect and impact peripheral neurons in vivo. Moreover, ZIKV productively infects stem-cell-derived human neural crest cells and peripheral neurons in vitro, leading to increased cell death, transcriptional dysregulation and cell-type-specific molecular pathology.
Show less
Date Issued
2017-09-01
Identifier
FSU_pmch_28758997, 10.1038/nn.4612, PMC5575960, 28758997, 28758997, nn.4612
Format
Citation
Structural Influences
Title
Structural Influences: Cholesterol, Drug, and Proton Binding to Full-Length Influenza A M2 Protein..
Creator
Ekanayake, E Vindana, Fu, Riqiang, Cross, Timothy A
Abstract/Description
The structure and functions of the M2 protein from Influenza A are sensitive to pH, cholesterol, and the antiinfluenza drug Amantadine. This is a tetrameric membrane protein of 97 amino-acid residues that has multiple functions, among them as a proton-selective channel and facilitator of viral budding, replacing the need for the ESCRT proteins that other viruses utilize. Here, various amino-acid-specific-labeled samples of the full-length protein were prepared and mixed, so that only...
Show moreThe structure and functions of the M2 protein from Influenza A are sensitive to pH, cholesterol, and the antiinfluenza drug Amantadine. This is a tetrameric membrane protein of 97 amino-acid residues that has multiple functions, among them as a proton-selective channel and facilitator of viral budding, replacing the need for the ESCRT proteins that other viruses utilize. Here, various amino-acid-specific-labeled samples of the full-length protein were prepared and mixed, so that only interresidue (13)C-(13)C cross peaks between two differently labeled proteins representing interhelical interactions are observed. This channel is activated at slightly acidic pH values in the endosome when the His(37) residues in the middle of the transmembrane domain take on a +2 or +3 charged state. Changes observed here in interhelical distances in the N-terminus can be accounted for by modest structural changes, and no significant changes in structure were detected in the C-terminal portion of the channel upon activation of the channel. Amantadine, which blocks proton conductance by binding in the aqueous pore near the N-terminus, however, significantly modifies the tetrameric structure on the opposite side of the membrane. The interactions between the juxtamembrane amphipathic helix of one monomer and its neighboring monomer observed in the absence of drug are disrupted in its presence. However, the addition of cholesterol prevents this structural disruption. In fact, strong interactions are observed between cholesterol and residues in the amphipathic helix, accounting for cholesterol binding adjacent to a native palmitoylation site and near to an interhelix crevice that is typical of cholesterol binding sites. The resultant stabilization of the amphipathic helix deep in the bilayer interface facilitates the bilayer curvature that is essential for viral budding.
Show less
Date Issued
2016-03-29
Identifier
FSU_pmch_27028648, 10.1016/j.bpj.2015.11.3529, PMC4816700, 27028648, 27028648, S0006-3495(16)00152-1
Format
Citation
Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids.
Title
Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids.
Creator
Kizziah, James L, Manning, Keith A, Dearborn, Altaira D, Wall, Erin A, Klenow, Laura, Hill, Rosanne L L, Spilman, Michael S, Stagg, Scott M, Christie, Gail E, Dokland, Terje
Abstract/Description
In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (SP) that serves as a chaperone for the assembly process. bacteriophage 80α is capable of high frequency mobilization of mobile genetic elements called pathogenicity islands (SaPIs), such as SaPI1....
Show moreIn the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (SP) that serves as a chaperone for the assembly process. bacteriophage 80α is capable of high frequency mobilization of mobile genetic elements called pathogenicity islands (SaPIs), such as SaPI1. SaPI1 redirects the assembly pathway of 80α to form capsids that are smaller than those normally made by the phage alone. Both CP and SP of 80α are N-terminally processed by a host-encoded protease, Prp. We have analyzed phage mutants that express pre-cleaved or uncleavable versions of CP or SP, and show that the N-terminal sequence in SP is absolutely required for assembly, but does not need to be cleaved in order to produce viable capsids. Mutants with pre-cleaved or uncleavable CP display normal viability. We have used cryo-EM to solve the structures of mature capsids from an 80α mutant expressing uncleavable CP, and from wildtype SaPI1. Comparisons with structures of 80α and SaPI1 procapsids show that capsid maturation involves major conformational changes in CP, consistent with a release of the CP N-arm by SP. The hexamers reorganize during maturation to accommodate the different environments in the 80α and SaPI1 capsids.
Show less
Date Issued
2017-12-16
Identifier
FSU_pmch_29258203, 10.3390/v9120384, PMC5744158, 29258203, 29258203, v9120384
Format
Citation
Kaposi's Sarcoma-Associated Herpesvirus Inhibitor of cGAS (KicGAS), Encoded by ORF52, Is an Abundant Tegument Protein and Is Required for Production of Infectious Progeny Viruses.
Title
Kaposi's Sarcoma-Associated Herpesvirus Inhibitor of cGAS (KicGAS), Encoded by ORF52, Is an Abundant Tegument Protein and Is Required for Production of Infectious Progeny Viruses.
Creator
Li, Wenwei, Avey, Denis, Fu, Bishi, Wu, Jian-Jun, Ma, Siming, Liu, Xia, Zhu, Fanxiu
Abstract/Description
Although Kaposi's sarcoma-associated herpesvirus (KSHV) ORF52 (also known as KSHV inhibitor of cGAS [KicGAS]) has been detected in purified virions, the roles of this protein during KSHV replication have not been characterized. Using specific monoclonal antibodies, we revealed that ORF52 displays true late gene expression kinetics and confirmed its cytoplasmic localization in both transfected and KSHV-infected cells. We demonstrated that ORF52 comigrates with other known virion proteins...
Show moreAlthough Kaposi's sarcoma-associated herpesvirus (KSHV) ORF52 (also known as KSHV inhibitor of cGAS [KicGAS]) has been detected in purified virions, the roles of this protein during KSHV replication have not been characterized. Using specific monoclonal antibodies, we revealed that ORF52 displays true late gene expression kinetics and confirmed its cytoplasmic localization in both transfected and KSHV-infected cells. We demonstrated that ORF52 comigrates with other known virion proteins following sucrose gradient centrifugation. We also determined that ORF52 resides inside the viral envelope and remains partially associated with capsid when extracellular virions are treated with various detergents and/or salts. There results indicate that ORF52 is a tegument protein abundantly present in extracellular virions. To characterize the roles of ORF52 in the KSHV life cycle, we engineered a recombinant KSHV ORF52-null mutant virus and found that loss of ORF52 results in reduced virion production and a further defect in infectivity. Upon analysis of the virion composition of ORF52-null viral particles, we observed a decrease in the incorporation of ORF45, as well as other tegument proteins, suggesting that ORF52 is important for the packaging of other virion proteins. In summary, our results indicate that, in addition to its immune evasion function, KSHV ORF52 is required for the optimal production of infectious virions, likely due to its roles in virion assembly as a tegument protein. The tegument proteins of herpesviruses, including Kaposi's sarcoma-associated herpesvirus (KSHV), play key roles in the viral life cycle. Each of the three subfamilies of herpesviruses (alpha, beta, and gamma) encode unique tegument proteins with specialized functions. We recently found that one such gammaherpesvirus-specific protein, ORF52, has an important role in immune evasion during KSHV primary infection, through inhibition of the host cytosolic DNA sensing pathway. In this report, we further characterize ORF52 as a tegument protein with vital roles during KSHV lytic replication. We found that ORF52 is important for the production of infectious viral particles, likely through its role in virus assembly, a critical process for KSHV replication and pathogenesis. More comprehensive investigation of the functions of tegument proteins and their roles in viral replication may reveal novel targets for therapeutic interventions against KSHV-associated diseases.
Show less
Date Issued
2016-05-12
Identifier
FSU_pmch_27009954, 10.1128/JVI.02675-15, PMC4934757, 27009954, 27009954, JVI.02675-15
Format
Citation

Pages