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- Title
- Assortative Mating in the Tropical Sea Urchin Lytechinus Variegatus.
- Creator
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Moscoso, Jose A., Department of Biological Science
- Abstract/Description
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Lytechinus variegatus, a widely known species of sea urchins, exist in different color morphs. Previous studies have shown that gamete recognition proteins are related to color expression in Echinoderms. In this study, crosses within and between color morphs of L. variegatus were done to assess assortative fertilization, and field measurements of aggregation and color frequency to determine behavioral and genetic components of assortative mating. Differences in fertilization success were...
Show moreLytechinus variegatus, a widely known species of sea urchins, exist in different color morphs. Previous studies have shown that gamete recognition proteins are related to color expression in Echinoderms. In this study, crosses within and between color morphs of L. variegatus were done to assess assortative fertilization, and field measurements of aggregation and color frequency to determine behavioral and genetic components of assortative mating. Differences in fertilization success were discovered between color morphs and evidence was found for color aggregation as well as phenotypic effects in the distribution of colors in a population of L. variegatus.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0490
- Format
- Thesis
- Title
- Asterisk Comics: Visual Rhetoric and Digital Media in Writing Instruction.
- Creator
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Salamanca, Valentina, Department of English
- Abstract/Description
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Asterisk* Comics seeks to provide visual and interactive tools through which young adults can develop their writing skills. The website's primary focus is to serve as a medium for instructional materials and to promote writing by marketing it in a new, fresh way. Up to this point, most writing advice offered online takes the form of a textbook pasted unto the web, and this means college and high school students have to weed through protracted explanations to have a simple question answered....
Show moreAsterisk* Comics seeks to provide visual and interactive tools through which young adults can develop their writing skills. The website's primary focus is to serve as a medium for instructional materials and to promote writing by marketing it in a new, fresh way. Up to this point, most writing advice offered online takes the form of a textbook pasted unto the web, and this means college and high school students have to weed through protracted explanations to have a simple question answered. Googling for writing instruction can become a tortuous experience for anyone, but Asterisk* Comics seeks to break this tedious trend. Asteriskcomics.org will house the same type of information offered by instructional writing websites, but they will be made available through interactive and visual means. Writing instruction and advice will be conveyed to its audience through comics and illustrated texts. It aspires to house videos and interactive media in the future.
Show less - Date Issued
- 2013
- Identifier
- FSU_migr_uhm-0239
- Format
- Thesis
- Title
- Attention Deficit/Hyperactivity Disorder (ADHD) and Kv1.3: Evaluating a Potential Disease Model.
- Creator
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Hoffman, Carlie, Department of Biological Science
- Abstract/Description
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Attention deficit/hyperactivity disorder (ADHD) is a condition characterized by excessive levels of impulsivity, inattention, and hyperactivity. Mice subjected to deletion of the Kv1.3 potassium ion channel (knockout, KO) were observed to display behavioral symptoms of ADHD, indicating they may model this disorder. Due to the comorbidity between ADHD and anxiety disorders, the anxiety levels of KO and wildtype (WT) mice were determined as a precursor for the presence of additional ADHD-type...
Show moreAttention deficit/hyperactivity disorder (ADHD) is a condition characterized by excessive levels of impulsivity, inattention, and hyperactivity. Mice subjected to deletion of the Kv1.3 potassium ion channel (knockout, KO) were observed to display behavioral symptoms of ADHD, indicating they may model this disorder. Due to the comorbidity between ADHD and anxiety disorders, the anxiety levels of KO and wildtype (WT) mice were determined as a precursor for the presence of additional ADHD-type behaviors using marble burying, elevated plus maze, and light-dark box testing. Because ADHD affects children and adults alike, inattentive and hyperactive behaviors were quantified for young and aged mice of both genotypes. Methylphenidate (MPH) or saline were also administered to the mice via oral gavage to determine the influence of MPH on behavior. Anxiety testing indicated that KO mice trended towards having decreased anxiety levels compared to their WT counterparts. Object-based attention testing indicated young and aged KO mice had attention deficits and treatment with MPH ameliorated this symptom. However, metabolic chamber testing revealed that WT and KO mice had equivalent activity levels and MPH treatment had no influence on locomotor activity. Based on these findings, there is a link between enhanced olfactory ability and decreased anxiety, and KO mice may be used as behavioral models of the inattentive subtype of ADHD.
Show less - Date Issued
- 2014
- Identifier
- FSU_migr_uhm-0316
- Format
- Thesis
- Title
- Auction IPOs: An Experimental Look.
- Creator
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Taylor, Brennan M
- Abstract/Description
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The main way IPOs are issued onto the market is through the underwriting process. This process requires that an investment bank be a middle-man and assume most of the risk while trying to sell IPOs. Most of the risk is whether or not the investment bank can raise the stated amount of capital in the contact with the firm, if not, they will eat the missing investments. This effectively allows the investment to underprice the IPO and in turn, make the firm lose potential investment while also...
Show moreThe main way IPOs are issued onto the market is through the underwriting process. This process requires that an investment bank be a middle-man and assume most of the risk while trying to sell IPOs. Most of the risk is whether or not the investment bank can raise the stated amount of capital in the contact with the firm, if not, they will eat the missing investments. This effectively allows the investment to underprice the IPO and in turn, make the firm lose potential investment while also gaining from the expenses charged to the firm. With the introduction of the Auction IPO it is intriguing as to why firms have not completely switched over to using markets instead of investment banks. The Auction IPO, in theory, gets rid of the ability to underprice IPOs. This would cut out the middle-man and limit the underpricing of IPOs, which allows the firm to extract most of its potential capital from investment. However, firms are not switching over from the underwriting process to the auction. This thesis attempts to help explain why firms are not switching while also using experimental methods to compare each system of issuing.
Show less - Date Issued
- 2016-12-09
- Identifier
- FSU_libsubv1_scholarship_submission_1481270748
- Format
- Thesis
- Title
- AUTHORITARIANISM IN AMERICAN POLITICAL BEHAVIOR & THE 2016 PRESIDENTIAL ELECTION.
- Creator
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Monell, Kevin Norberto
- Abstract/Description
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This paper analyzes what conditions led to the election of Donald Trump as the 45th president of the United States. The introduction sets the stage for the research question by examining the Trump campaign in the months leading to Election Day. Examination of the campaign’s rhetoric and Trump’s actions indicates that Trump tapped into the electorate’s authoritarian attitudes. Incorporating a measure of authoritarian dispositions that is valid among white non-Hispanic Americans, six linear...
Show moreThis paper analyzes what conditions led to the election of Donald Trump as the 45th president of the United States. The introduction sets the stage for the research question by examining the Trump campaign in the months leading to Election Day. Examination of the campaign’s rhetoric and Trump’s actions indicates that Trump tapped into the electorate’s authoritarian attitudes. Incorporating a measure of authoritarian dispositions that is valid among white non-Hispanic Americans, six linear regression models were estimated to test the hypothesis that those having a high authoritarian disposition were more likely to vote for Donald Trump. The results suggest that having a high authoritarian disposition made a white non-Hispanic voter more likely to vote for Donald Trump than Hillary Clinton.
Show less - Date Issued
- 2018-04-02
- Identifier
- FSU_libsubv1_scholarship_submission_1522782841_9efae325
- Format
- Thesis
- Title
- Auto-Tune in Africa: A Study of the Factors Leading to the Phenomenon.
- Creator
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Stanley, Andrew, College of Music
- Abstract/Description
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Auto-Tune is a fascinating phenomenon in the modern music industry. It has gained a large amount of popularity over the past ten years, and it is constantly heard in popular music hits and on the radio. However, in most discussions involving music, Auto-Tune is often regarded as a novelty, or as something that serious musicians do not bother using. This stigma has followed it into scholarship, where there has been little to no study of its impact. Auto-Tune's use is not only limited to the...
Show moreAuto-Tune is a fascinating phenomenon in the modern music industry. It has gained a large amount of popularity over the past ten years, and it is constantly heard in popular music hits and on the radio. However, in most discussions involving music, Auto-Tune is often regarded as a novelty, or as something that serious musicians do not bother using. This stigma has followed it into scholarship, where there has been little to no study of its impact. Auto-Tune's use is not only limited to the United States. It is particularly popular throughout the African continent, which is the region I chose to focus on in this thesis. This thesis seeks to answer why Auto-Tune has become so popular in modern African popular music, and to provide evidence of said popularity. I believe that this question can be answered by looking at three facets of modern society. First, Auto-Tune appears to have become a globalized commodity that links and unifies different regions and the mindsets that utilize it. Additionally, the music industry has been working towards a goal of near total control over music production since its creation, and the influence from the large record companies around the world has grown over the last ten years, leading to a dispersal of this mindset, in form of a musical "standard" of production. Furthermore, Auto-Tune presents itself with a number of qualities that appeal to the musical mentality that exists throughout the African continent. These qualities reflect the importance of community and language, a variety of technical features reflected in the music, and also fit into the established history of hybridity within the African continent.
Show less - Date Issued
- 2012
- Identifier
- FSU_migr_uhm-0127
- Format
- Thesis
- Title
- Autocorrect Awareness: Categorizing Autocorrect Changes and Measuring Authorial Perceptions.
- Creator
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Wood, Nicola, Department of English
- Abstract/Description
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This thesis studies changes made by Autocorrect software and authorial awareness and perceptions of those changes through analysis of case studies conducted on five volunteers aged 19 to 22. The study consisted of two phases: 1. three writing tasks, and 2. a post-writing survey given to the authors. For the first task, each subject completed three predetermined writing prompts: an email message, a text message, and a Facebook status—each with a specific intended audience—on their iPhones....
Show moreThis thesis studies changes made by Autocorrect software and authorial awareness and perceptions of those changes through analysis of case studies conducted on five volunteers aged 19 to 22. The study consisted of two phases: 1. three writing tasks, and 2. a post-writing survey given to the authors. For the first task, each subject completed three predetermined writing prompts: an email message, a text message, and a Facebook status—each with a specific intended audience—on their iPhones. Subjects then completed the survey to self-report their level of awareness of AutoCorrect's changes. Correction data was coded and categorized into one of four types of changes. The change type was determined by analyzing video recordings of each prompt. Type B changes—made when the subject accidentally hit the wrong key—proved to be the most common, followed by Type A changes (made when the subject seemed unable to spell the word), Type C changes (made as a result of incorrect capitalization or punctuation), and Type D changes (changes that did not fit into another category). Four out of five subjects self-reported that AutoCorrect changed their writing over 10 times. The fifth participant reported that AutoCorrect made 6-10 changes, though the program had actually altered his work 18 times. His response suggests that AutoCorrect may be becoming invisible to some users. The observations in this thesis are not generalizable; instead, they serve to provide a starting point for further exploration into authorial awareness in digital writing contexts.
Show less - Date Issued
- 2014
- Identifier
- FSU_migr_uhm-0351
- Format
- Thesis
- Title
- Automated Analysis of Protein Side Chain Spectra.
- Creator
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Hart, Andrew, Department of Chemistry and Biochemistry
- Abstract/Description
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Manual Nuclear Magnetic Resonance (NMR) spectral analysis of proteins is a time intensive effort with methods often specific to each analysis. The method described in this thesis automates the resonance assignment of protein side chains using a TOCSY (Totally Correlated Spectroscopy) NMR experiment. The system under study is Ubiquitin (8.6 kDa). 54 of the 70 available amino acid side chains were identified by a single TOCSY spectrum in less than 5 min of local computer runtime using the...
Show moreManual Nuclear Magnetic Resonance (NMR) spectral analysis of proteins is a time intensive effort with methods often specific to each analysis. The method described in this thesis automates the resonance assignment of protein side chains using a TOCSY (Totally Correlated Spectroscopy) NMR experiment. The system under study is Ubiquitin (8.6 kDa). 54 of the 70 available amino acid side chains were identified by a single TOCSY spectrum in less than 5 min of local computer runtime using the algorithms described. Automation of spectral analysis can enhance reproducibility and create standards of spectral analysis.
Show less - Date Issued
- 2013
- Identifier
- FSU_migr_uhm-0244
- Format
- Thesis
- Title
- Autonomous RC Aircraft with Collision Avoidance Capabilities.
- Creator
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Timmons, Cristopher, Department of Electrical and Computer Engineering
- Abstract/Description
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This thesis directly deals with setting up all of the electrical components to create an inexpensive automatic dependent surveillance broadcast (ADS-B) receiver that can transmit the ADS-B data received to a ground control station (GCS) using a Raspberry Pi (single chip computer), operating an autopilot system from the same GCS, and sending the telemetry data to and from the autopilot system and the GCS also using the Raspberry Pi. Furthermore, it will discuss how this setup allows the system...
Show moreThis thesis directly deals with setting up all of the electrical components to create an inexpensive automatic dependent surveillance broadcast (ADS-B) receiver that can transmit the ADS-B data received to a ground control station (GCS) using a Raspberry Pi (single chip computer), operating an autopilot system from the same GCS, and sending the telemetry data to and from the autopilot system and the GCS also using the Raspberry Pi. Furthermore, it will discuss how this setup allows the system to be capable to performing autonomous collision avoidance maneuvers. Finally, it will show how these capabilities can be demonstrated in a Hardware-in-the-Loop Simulation (HILSim). As the member in charge of performing autonomous flight for the Autonomous Aerial Vehicle senior design team last year, it was ideal to continue research in the field of autonomous flight. The original goal was to compete in the Unmanned Air Systems (UAS) Airspace Operations Challenge (AOC) NASA Centennial Challenge, however, not enough funds were acquired to enter this competition. Therefore, the focus of the thesis shifted to setting up the electrical components of an RC aircraft that would be able to meet the general requirements of the competition.
Show less - Date Issued
- 2014
- Identifier
- FSU_migr_uhm-0338
- Format
- Thesis
- Title
- Becoming American.
- Creator
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Amar, Monika, Program in International Affairs
- Abstract/Description
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This thesis examines various aspects of modern American culture and addresses potential causes and effects of American individualism and of the current mainstream American family dynamic. Discussing the effects that Americanism can have on interaction within immigrant families, as well as on society at large via globalization, this thesis evaluates historical, political and technological aspects of American society, which attribute to present-day functioning of individuals living in America...
Show moreThis thesis examines various aspects of modern American culture and addresses potential causes and effects of American individualism and of the current mainstream American family dynamic. Discussing the effects that Americanism can have on interaction within immigrant families, as well as on society at large via globalization, this thesis evaluates historical, political and technological aspects of American society, which attribute to present-day functioning of individuals living in America and beyond. Attributing changes in the way people communicate within the family realm and with others, this thesis analyzes the detrimental effects of overuse and dependence on information and communication technologies — mainly the Internet and the mobile phone — as well as the diffusion of capitalism into various realms of American society and even on a global scale as humans have become more interconnected. The intention of this thesis is to highlight the developing elements of mainstream American culture and to expose the potential for a worldwide community with the increased progressive industrialization and urbanization of more and more countries.
Show less - Date Issued
- 2014
- Identifier
- FSU_migr_uhm-0337
- Format
- Thesis
- Title
- Behavioral Assessment of Finger-Counting on SNARC.
- Creator
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Gonzalez, Nicole A., Kowalsky, Amanda L., Kaschak, Michael P., Department of Psychology
- Abstract/Description
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Individuals tend to exhibit implicit, cognitive associations between numbers and space. Small numbers become associated with the left side of their bodies and large numbers with the right side of their bodies. This "spatial-numerical association of response codes" (SNARC) provides evidence that individuals tend to sort their spatial orientations along a mental number line. Since most cultures promote the use of finger-counting as a universal means for learning to work with numbers, it is...
Show moreIndividuals tend to exhibit implicit, cognitive associations between numbers and space. Small numbers become associated with the left side of their bodies and large numbers with the right side of their bodies. This "spatial-numerical association of response codes" (SNARC) provides evidence that individuals tend to sort their spatial orientations along a mental number line. Since most cultures promote the use of finger-counting as a universal means for learning to work with numbers, it is believed that the directionality of finger-counting (from left to right or right to left) affects the way we link numbers and space in adulthood. To assess finger-directionality, past studies have utilized self-report questionnaires; however, recent findings have suggested a new measure that classifies finger-directionality by observing natural finger-counting habits and circumvents the biases associated with self-report. In the current study with a sample of ninety-four college students, when using self-report to categorize counting habits, we found a statistically significant difference between groups; right-starters displayed the SNARC effect while left-starters did not. However, when using observed behaviors to categorize counting habits, we did not find a statistically significant difference between left and right starters. These findings suggest that finger-counting hands do not predict the SNARC effect, which is consistent with the flexibility of the effect itself.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0581
- Format
- Thesis
- Title
- Behavioral Implications of Growth and Destiny Beliefs in Romantic Relationships.
- Creator
-
Montalvo, Shae, McNulty, James, Hicks, Lindsey
- Abstract/Description
-
This study tested the association between romantic partners’ growth beliefs and their use of negative direct communication strategies, rather than negative indirect communication strategies, when discussing problems in their relationship. Because of the benefits associated with the use of direct strategies when discussing relationship problems (i.e., clarification of the problem, motivation to resolve problem), we predicted that spouses with growth beliefs would be more likely to use direct...
Show moreThis study tested the association between romantic partners’ growth beliefs and their use of negative direct communication strategies, rather than negative indirect communication strategies, when discussing problems in their relationship. Because of the benefits associated with the use of direct strategies when discussing relationship problems (i.e., clarification of the problem, motivation to resolve problem), we predicted that spouses with growth beliefs would be more likely to use direct communication strategies during conflict discussions. Ultimately, we predicted that the use of direct communication strategies would result in higher relationship satisfaction for spouses with a growth mindset. These predictions were tested using a behavioral study of 120 couples, during which newlywed spouses reported their implicit theories about relationships and participated in conflict discussions. Moderated mediational analyses revealed that spouses with growth beliefs (compared to spouses with destiny beliefs) were not more likely to employ direct communication strategies during conflict discussions. Moreover, the use of direct strategies did not account for any differences in relationship satisfaction between partners with fixed and growth mindsets.
Show less - Date Issued
- 2017-04-28
- Identifier
- FSU_libsubv1_scholarship_submission_1493410662
- Format
- Thesis
- Title
- Behavioral Reactivity to Social Defeat Stress after Nicotine Exposure during Adolescence in C57/BL6 Male Mice.
- Creator
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Duperrouzel, Jacqueline, Department of Psychology
- Abstract/Description
-
Nicotine use during adolescence is associated with the development of many debilitating pathologies in adulthood such as anxiety and depression. Adolescence is a time of many social pressures and biological changes occurring within the brain and body. Therefore, studying the effects of nicotine exposure during adolescence on stress and subsequent sensitivity to nicotine itself is of great interest. To examine these questions I designed experiments to assess both short (i.e., 24 hours after...
Show moreNicotine use during adolescence is associated with the development of many debilitating pathologies in adulthood such as anxiety and depression. Adolescence is a time of many social pressures and biological changes occurring within the brain and body. Therefore, studying the effects of nicotine exposure during adolescence on stress and subsequent sensitivity to nicotine itself is of great interest. To examine these questions I designed experiments to assess both short (i.e., 24 hours after treatment) and long-term (i.e., four weeks after treatment) behavioral consequences of nicotine (0.32 mg/kg) exposure during adolescence (i.e., postnatal days 35-49) in C57/BL6 male mice by exposing them to social-defeat stress, social interaction, and subsequent preference for nicotine. Nicotine-treated mice in the short-term condition displayed avoidant behaviors and a preference for the lowest concentration of nicotine (5 mg/l). Interestingly, the nicotine-treated mice in the long-term condition displayed enhanced social avoidance as well, with a similar preference for the low concentration of nicotine. These results indicate that exposure to nicotine during adolescence influences social interaction behavior and increases preference for nicotine, findings that last into adulthood. Future studies using different stressors and a complete nicotine dose-response may reveal further insight into how nicotine affects this age group and its long-lasting consequences. The work presented here increases our understanding of the effects of nicotine exposure during adolescents and the later effects, which may develop over time.
Show less - Date Issued
- 2013
- Identifier
- FSU_migr_uhm-0194
- Format
- Thesis
- Title
- Behind the Numbers: Voices of Gorubathan: Artist Statement.
- Creator
-
Miller, Savannah Gale
- Abstract/Description
-
The film explores gender and education inequalities in the village of Gorubathan in the Darjeeling district of West Bengal, India by documenting the experience of two young women as they navigate traditional gender roles and higher education. The artist statement adds contextualization for the paradox of gender equality presented by international development organizations that use Western liberal ideology to define gender equality, and simultaneously discriminate against gender roles that...
Show moreThe film explores gender and education inequalities in the village of Gorubathan in the Darjeeling district of West Bengal, India by documenting the experience of two young women as they navigate traditional gender roles and higher education. The artist statement adds contextualization for the paradox of gender equality presented by international development organizations that use Western liberal ideology to define gender equality, and simultaneously discriminate against gender roles that were not supportive of the Western definition. Additionally, the artist statement gives a brief history of ethnographic films impact on anthropological knowledge and representation for marginalized communities. Because reflexivity is vital for capturing the emic perspective, the film discusses the construction and evolution of the film in order to bring visibility to the sociocultural complexities of gender and education in Gorubathan undermined by international organizations aimed at fostering development.
Show less - Date Issued
- 2017-12-04
- Identifier
- FSU_libsubv1_scholarship_submission_1512684949_f40743fc_P
- Format
- Set of related objects
- Title
- Belongingness and Suicidal Ideation Among Hispanic/Latino Individuals.
- Creator
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Acosta, Laura, Department of Psychology
- Abstract/Description
-
The interpersonal theory of suicide states that thwarted belongingness, a perception of social isolation and lack of social connectedness, is an important factor related to the experience of suicidal ideation. A strong familial connection, characteristic of Hispanic/Latino culture, is associated with lower levels of thwarted belongingness. In this study, thwarted belongingness and ethnicity were used to predict lifetime levels of suicidal ideation among Hispanic/Latino and non-Hispanic white...
Show moreThe interpersonal theory of suicide states that thwarted belongingness, a perception of social isolation and lack of social connectedness, is an important factor related to the experience of suicidal ideation. A strong familial connection, characteristic of Hispanic/Latino culture, is associated with lower levels of thwarted belongingness. In this study, thwarted belongingness and ethnicity were used to predict lifetime levels of suicidal ideation among Hispanic/Latino and non-Hispanic white undergraduate college students. Based on previous research and their typically high value placed on family, Hispanics were predicted to experience lower levels of suicidal ideation and thwarted belongingness. Thwarted belongingness as well as Hispanic ethnicity were hypothesized to significantly predict suicidal ideation. Additionally, Hispanic status was hypothesized to moderate the role of thwarted belongingness such that Hispanics who reported high levels of thwarted belongingness would experience the highest levels of suicidal ideation. Undergraduate college students, of whom 22% were Hispanic/Latino and 78% were non-Hispanic white (N = 170), completed self-reported measures. Thwarted belongingness, but not ethnicity, significantly predicted intensity of lifetime suicidal ideation. Hispanic/Latino status did not have an effect on suicidal ideation and was not a moderator of the effect of thwarted belongingness. These findings highlight thwarted belongingness as a key factor for assessing suicidal ideation in college students.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0489
- Format
- Thesis
- Title
- The Best Laid Plans, Laid to Rest: The Fall of the Florida Department of Community Affairs.
- Creator
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Medin, Kyle A., Department of Political Science
- Abstract/Description
-
Florida Governor Rick Scott defended his move to eliminate the growth management-oriented Department of Community Affairs (DCA) in 2011, claiming that the DCA was killing jobs in the state of Florida, and that it was an inefficient use of Government money. This paper set out to test Governor Scott's claims against the data, to see if there was evidence to support his assertions. The relationship between DCA's presence and three employment variables (monthly construction jobs, aggregate jobs,...
Show moreFlorida Governor Rick Scott defended his move to eliminate the growth management-oriented Department of Community Affairs (DCA) in 2011, claiming that the DCA was killing jobs in the state of Florida, and that it was an inefficient use of Government money. This paper set out to test Governor Scott's claims against the data, to see if there was evidence to support his assertions. The relationship between DCA's presence and three employment variables (monthly construction jobs, aggregate jobs, unemployment rate) was tested with multivariate linear regression models. DCA's effect on job growth was tested by comparing job growth data from Florida with the corresponding data from Texas (a state without any such growth management agency) and the national average (to contextualize the states' growth), using paired t-tests. The effectiveness of the DCA at regulating housing growth was similarly tested, by comparing data on housing growth rates and housing density from Florida with the corresponding data from Texas and the national average using paired t-tests. The majority of the tests refuted Gov. Scott's claims. This paper lends support to the proponents of growth management, using a novel approach.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0567
- Format
- Thesis
- Title
- Beverage Consumption in Children with Diabetes Mellitus: Weekend Versus Weekday Intake.
- Creator
-
Purcell, Sarah, Nutrition, Food, & Exercise Science
- Abstract/Description
-
Although high sugar sweetened beverage (SSB) intake in children is strongly correlated to adverse health consequences, the relationship between weekend and weekday beverage consumption lacks sufficient research. Specifically, SSB intake patterns in children with Type 1 diabetes mellitus (DM) have not been thoroughly explored. Thus, the goal of this research was to examine the type and frequency of SSBs consumed by children on weekdays versus the weekends. Data were gathered from Dr. Suzanne...
Show moreAlthough high sugar sweetened beverage (SSB) intake in children is strongly correlated to adverse health consequences, the relationship between weekend and weekday beverage consumption lacks sufficient research. Specifically, SSB intake patterns in children with Type 1 diabetes mellitus (DM) have not been thoroughly explored. Thus, the goal of this research was to examine the type and frequency of SSBs consumed by children on weekdays versus the weekends. Data were gathered from Dr. Suzanne Bennett-Johnson's Diabetes Adherence grant using children that were undergoing treatment at a diabetes clinic either in Gainesville or Tallahassee, Florida. After voiding certain entries, the final study population consisted of 67 children with Type 1 DM age 6-13 (8.7 ± 1.9); of those, 22 participants consumed beverages and their intake was examined. Diet records were output from Nutrition Data Systems for Research (NDSR) and input into Microsoft Excel where two-sample T-tests were utilized. Our results showed that when fruit beverages and regular sodas were combined into one category, children drank more on the weekday than on the weekend (P=0.021). Upon isolation, fruit drinks were overwhelmingly consumed more often (P=0.028) on the weekdays than on the weekends, while soda consumption variances were insignificant (P=0.445). Age or sex did not have a significant impact on servings of beverages, although there were correlations between race and intake. Though dietary intake is influenced by many factors, our findings suggest that attention needs to be placed on educating children, especially those with Type 1 DM, and their families about beverage choice.
Show less - Date Issued
- 2012
- Identifier
- FSU_migr_uhm-0105
- Format
- Thesis
- Title
- Bilingual Education in Florida: Effect of Bilingual Education in Florida.
- Creator
-
Perla, Meylin C.
- Abstract/Description
-
The purpose of this thesis is to determine the impact of bilingual education in the state of Florida on students’ academic performance. In order to procure an unbiased assessment of the effects of bilingual education, the reading and mathematics FCAT 2.0 scores for the years 2011 to 2014 in two bilingual, two partially bilingual, and two monolingual K-8 schools will be examined. The goal is to determine if program type has an effect on passing rates. For the purpose of this thesis it was...
Show moreThe purpose of this thesis is to determine the impact of bilingual education in the state of Florida on students’ academic performance. In order to procure an unbiased assessment of the effects of bilingual education, the reading and mathematics FCAT 2.0 scores for the years 2011 to 2014 in two bilingual, two partially bilingual, and two monolingual K-8 schools will be examined. The goal is to determine if program type has an effect on passing rates. For the purpose of this thesis it was expected that bilingual education does not negatively affect the performance of students in FCAT 2.0 reading and mathematics. The results show that two-way immersion program has a positive impact on academic performance in the reading portion of standardized assessments. The type of program does not appear to have an effect on students’ performance in the mathematics assessment.
Show less - Date Issued
- 2016-04-20
- Identifier
- FSU_libsubv1_scholarship_submission_1461133689
- Format
- Thesis
- Title
- Biocompatible Poly (Lactic Acid)/Thermoplastic Polyurethane Blends.
- Creator
-
Burkett, Mary K., Department of Industrial and Manufacturing Engineering
- Abstract/Description
-
In order to create a polymer that has the morphology and structure to be used for industrial applications, the foamability of the PLA must be improved by blending. Blends were made in specific ratios of 80/20, 50/50, and 20/80. Next, the blends were extruded and injected into molds for testing. The mechanical and thermal properties of the blends were tested using the tensile test and the DSC. The morphology was also observed using the SEM. Finally, a series of stock materials were created to...
Show moreIn order to create a polymer that has the morphology and structure to be used for industrial applications, the foamability of the PLA must be improved by blending. Blends were made in specific ratios of 80/20, 50/50, and 20/80. Next, the blends were extruded and injected into molds for testing. The mechanical and thermal properties of the blends were tested using the tensile test and the DSC. The morphology was also observed using the SEM. Finally, a series of stock materials were created to be used for melt-electrospinning or 3D printing.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0526
- Format
- Thesis
- Title
- Biophysical Characterization of a ssDNA Virus.
- Creator
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Morrison, Anneliese J., Department of Chemistry and Biochemistry
- Abstract/Description
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Viral capsids must assemble into stable structures that resist dissociation in extreme environments between hosts yet they simultaneously must be unstable enough to release their genome upon infection. The conflicting functions that the viral capsid must fulfill suggests that they exhibit an evolutionarily fine-tuned structure/function relationship that is not apparent in many other systems. Biophysical characterization of viral assembly and disassembly processes can aid in developing an...
Show moreViral capsids must assemble into stable structures that resist dissociation in extreme environments between hosts yet they simultaneously must be unstable enough to release their genome upon infection. The conflicting functions that the viral capsid must fulfill suggests that they exhibit an evolutionarily fine-tuned structure/function relationship that is not apparent in many other systems. Biophysical characterization of viral assembly and disassembly processes can aid in developing an understanding of the physical mechanisms that underlie the relationship between tightly linked phenotypes in complex protein systems. In this honor's thesis project, the dissociation process was biophysically characterized in an ssDNA bacteriophage. After the development of a PEG precipitation based purification method, intrinsic fluorescence spectroscopy, static light scattering, and plaque assays were used to develop a two-step model that describes the molecular events that occur during Microvirid bacteriophage capsid dissociation. At 57˚C using a scan rate of 1˚C/min, loss of 99% of viral activity is observed corresponding to loss of the major spike protein. At 69˚C transitions are seen in fluorescence and light scattering spectra that indicate a structural change is occurring. Plaque assays confirm that immediately after the structural transition occurs all viral activity is lost, indicating that this second step represents global capsid dissociation.
Show less - Date Issued
- 2014
- Identifier
- FSU_migr_uhm-0428
- Format
- Thesis
- Title
- Biopsychosocial Prenatal Predictors of Postpartum Depression.
- Creator
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Uhl, Aubree A., Department of Psychology
- Abstract/Description
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Postpartum depression affects up to 15% of women, is highly disabling, but under-addressed in obstetrics settings. The objectives of this study were to examine the characteristics of women seeking obstetrical services in a health system serving primarily Medicaid patients, and to examine biopsychosocial predictors of postpartum depression (PPD) that may be routinely assessed during obstetrical visits. Specific variables examined included a previous history of depression, maternal behavioral...
Show morePostpartum depression affects up to 15% of women, is highly disabling, but under-addressed in obstetrics settings. The objectives of this study were to examine the characteristics of women seeking obstetrical services in a health system serving primarily Medicaid patients, and to examine biopsychosocial predictors of postpartum depression (PPD) that may be routinely assessed during obstetrical visits. Specific variables examined included a previous history of depression, maternal behavioral and health factors, and pregnancy complications. Multivariate analyses showed that prepartum depression, white race, and street drug use were significantly associated with postpartum depression. These findings can be used to help obstetricians target women with these risk factors and get them help before the onset of postpartum depression.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0448
- Format
- Thesis
- Title
- The Bird Show.
- Creator
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Valdespino, Amanda, Department of English
- Abstract/Description
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This thesis seeks to examine identity within a variety of topics. The author uses themes such as family, pop-culture, relationships, and fairy tales to form a personal identity in a narrative dominated poetry style.
- Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0482
- Format
- Thesis
- Title
- Bisphenol-A as an estrogen mimic altering chromatin structure: Link between environmental toxins and chromatin biochemical state.
- Creator
-
Budine, Taylor D
- Abstract/Description
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Steroid hormones are the largest class of regulatory factors in the mammalian biology. Steroid hormones bind hormone receptors, allowing them to dimerize and function as transcription factors. Frequently this regulation of gene transcription is associated with nucleosome redistribution. Bisphenol-A (BPA) is a common toxin found in many of the plasticids and epoxy resins we use every day. BPA is a thought to be an estradiol mimic, binding to hormone receptors allowing them to dimerize and...
Show moreSteroid hormones are the largest class of regulatory factors in the mammalian biology. Steroid hormones bind hormone receptors, allowing them to dimerize and function as transcription factors. Frequently this regulation of gene transcription is associated with nucleosome redistribution. Bisphenol-A (BPA) is a common toxin found in many of the plasticids and epoxy resins we use every day. BPA is a thought to be an estradiol mimic, binding to hormone receptors allowing them to dimerize and function as transcription factors, which stimulate nucleosome redistribution at inappropriate times. However, nucleosome distribution from BPA exposure has not yet been observed. I expect that as BPA mimics estradiol and dysregulates the potential for gene expression, and that monitoring alterations in chromatin structure will give valuable insights into the first steps of this dysregulation. I mapped nucleosome redistribution and nucleosome sensitivity in both untreated cells and cells exposed to BPA to observe any change in nucleosome repositioning and biochemical composition after this stimulus. If BPA functionally acts as an estradiol mimic and is able to change chromatin structure, untimely or unwanted gene expression may result. BPA will be expected to induce remolding in the cell’s chromatin structure and affect the endocrine responsive genes. My results would show how other stimuli could mimic naturally occurring substances and be unexpected regulatory factors in altering our chromatin structure. BPA is one of many persistent toxins readily in our environment that we are exposed to. We found that there are transient nucleosome positional changes with exposure to Estradiol and Bisphenol-A at the -2, -1, TSS, +1 and +2 positions while comparing the two stimuli at the 10-minute post exposure time period. We are especially interested in the nucleosome loss or gain at the +1 position because of its implications for possible gene expression. These results show that there is indeed similar chromatin remodeling behavior between Estradiol and Bisphenol-A to possibly warrant connections to the potential of the cell. This study will be one of the first to propose chromatin remodeling as the underpinnings to Bisphenol-A ability to mimic the natural sex hormone Estradiol. The scientific world is now becoming more aware of how environmental factors and pollutants all around us can have an acute or trans-generational effect on our health and epigenomic regulation.
Show less - Date Issued
- 2018-04-27
- Identifier
- FSU_libsubv1_scholarship_submission_1524847528_f54529a4
- Format
- Thesis
- Title
- BladeFoil - Evidence for a Common Ancestor Between the Beta-Trefoil and Beta-Propeller Protein Architectures.
- Creator
-
Parker, Joseph Brandon
- Abstract/Description
-
Proteins belong to different architectures (or “folds”) by the way they fold into their tertiary structure. Two common protein architectures are the beta-trefoil fold and the beta-propeller fold. The designed protein “Symfoil-4P” is an example of the beta-trefoil fold and exhibits symmetry in it’s three domains down to the primary level. When a single domain was isolated from Symfoil-4P and studied computationally, it was predicted to fold as a “blade” motif belonging to the beta-propeller...
Show moreProteins belong to different architectures (or “folds”) by the way they fold into their tertiary structure. Two common protein architectures are the beta-trefoil fold and the beta-propeller fold. The designed protein “Symfoil-4P” is an example of the beta-trefoil fold and exhibits symmetry in it’s three domains down to the primary level. When a single domain was isolated from Symfoil-4P and studied computationally, it was predicted to fold as a “blade” motif belonging to the beta-propeller protein methylamine dehydrogenase heavy chain (1MDA). This finding gave rise to the idea that there could be a common ancestor between these two protein architectures. The purpose of this study was to see if evidence could be provided for this common ancestor. To test this a blade sequence substitution was made in Symfoil-4P in two locations. Along with two blade insertions, 3 leucine point mutations in two of the mutants were made, creating four mutants in total. The goal of this study was to see if any of these four mutants fold. All four mutants were resolved using size exclusion chromatography (SEC) in both the presence and absence of guanidine hydrochloride (GuHCl), a chaotropic agent. Two of the mutants were characterized using differential scanning calorimetry (DSC) to test for thermostability and folding. SEC GuHCl experiments all showed significant unfolding events. DSC experiments showed evidence of unfolding events and high thermostability. The outcome from these experiments offers evidence for a possible common ancestor between these two protein architectures.
Show less - Date Issued
- 2018-04-22
- Identifier
- FSU_libsubv1_scholarship_submission_1524438081_15d64dbc
- Format
- Thesis
- Title
- Blake's and Shelley's Reader Responses to Milton's Satan in Paradise Lost.
- Creator
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Noud, Jennifer, Department of English
- Abstract/Description
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This study surveys William Blake's and Percy Bysshe Shelley's reader responses of Satan in John Milton's Paradise Lost. Blake and Shelley were both Romanticists and were highly captivated with the character of Satan. Their critiques of Milton's Satan are evident through their works. Blake's works that are examined are "The Marriage of Heaven and Hell," an eleven-page poem, Milton, an epic poem, and the illuminated printings of Milton's Paradise Lost. Shelley's works that are studied are...
Show moreThis study surveys William Blake's and Percy Bysshe Shelley's reader responses of Satan in John Milton's Paradise Lost. Blake and Shelley were both Romanticists and were highly captivated with the character of Satan. Their critiques of Milton's Satan are evident through their works. Blake's works that are examined are "The Marriage of Heaven and Hell," an eleven-page poem, Milton, an epic poem, and the illuminated printings of Milton's Paradise Lost. Shelley's works that are studied are Prometheus Unbound, a closet lyrical drama, and "A Defense of Poetry" which is an essay. Blake and Shelley believed that Satan was the proper hero of Milton's Paradise Lost. They both critiqued Milton's Satan by finding several imperfections in Paradise Lost. Both tried to surpass Milton by creating their own perfect version of Milton's Satan. Shelley goes a step beyond Blake when designing his Satan by producing a new tragic hero that does not have a hamartia.
Show less - Date Issued
- 2013
- Identifier
- FSU_migr_uhm-0234
- Format
- Thesis
- Title
- Bmh1/Bmh2 Redundancy in the Control of the Spindle Assembly Checkpoint in Yeast Cells.
- Creator
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Jones, Nat
- Abstract/Description
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The spindle assembly checkpoint (SAC) monitors errors in chromosome attachment, but the regulation of the SAC is not fully understood. Previous research shows that the budding yeast 14-3-3 protein Bmh1 serves a sequestering function for Fin1, which prevents the kinetochore recruitment of Fin1-PP1 and prolongs the SAC. After chromosome segregation, Bmh1 detaches from Fin1, allowing the Fin1-PP1complex to bind to the kinetochore and remove SAC proteins from the kinetochore. bmh1 mutant cells...
Show moreThe spindle assembly checkpoint (SAC) monitors errors in chromosome attachment, but the regulation of the SAC is not fully understood. Previous research shows that the budding yeast 14-3-3 protein Bmh1 serves a sequestering function for Fin1, which prevents the kinetochore recruitment of Fin1-PP1 and prolongs the SAC. After chromosome segregation, Bmh1 detaches from Fin1, allowing the Fin1-PP1complex to bind to the kinetochore and remove SAC proteins from the kinetochore. bmh1 mutant cells show chromosome missegregation and early dephosphorylation of SAC proteins in the presence of tension defects, which indicates premature anaphase entry. We found that Bmh2, a homologue of Bmh1 that is less prevalent within cells, has some redundant function with Bmh1. By creating a bmh1 GAL-BMH2 cell line, we were able to overexpress the Bmh2 protein in the absence of Bmh1. This new strain grew poorly on glucose medium when Bmh2 expression is suppressed, which confirmed the synthetic growth defect of the bmh1 bmh2 double mutant. When this strain was grown in galactose medium to induce Bmh2 overexpression, we observed suppressed sensitivity to microtubule poison and decreased Fin1 kinetochore localization. Therefore, we concluded that Bmh1 and Bmh2 play redundant roles in cell cycle regulation.
Show less - Date Issued
- 2018-04-27
- Identifier
- FSU_libsubv1_scholarship_submission_1524851794_9af3c0e6
- Format
- Thesis
- Title
- The Bolshevik Party on Women's Rights and Equality 1917-1920.
- Creator
-
Beck, Elizabeth, History
- Abstract/Description
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This thesis deals with the opinions and views regarding women's rights and equality by the Bolshevik Party in Russia during the period of 1917 to 1920. This study examines the Bolshevik party's rhetoric involving gender issues, primarily that of women, and the proposed methods of creating a better environment for women. This work relies heavily on Vladimir Lenin and Alexandra Kollontai's speeches and writings which express their views on women's equality and the rights of women. It also...
Show moreThis thesis deals with the opinions and views regarding women's rights and equality by the Bolshevik Party in Russia during the period of 1917 to 1920. This study examines the Bolshevik party's rhetoric involving gender issues, primarily that of women, and the proposed methods of creating a better environment for women. This work relies heavily on Vladimir Lenin and Alexandra Kollontai's speeches and writings which express their views on women's equality and the rights of women. It also relies on the writing of Louise Bryant, a woman from the United States who was present during the Bolshevik revolution for the initial six months, as she expresses her own opinions on the work of the Bolshevik party regarding women's rights. This thesis addresses the initial steps made by the Bolshevik party towards liberating women and creating gender equality during 1917-1920, but primarily deals with the ideas expressed regarding the issue.
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0004
- Format
- Thesis
- Title
- Breivik's Sanity: Historical and Contemporary Right-Wing Political Violence in Norway.
- Creator
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Jacobsen, Colin, College of Criminology and Criminal Justice
- Abstract/Description
-
On July 22, 2011 Anders Behring Breivik bombed a government building in Oslo, resulting in the deaths of eight people. A few hours later he attacked a youth camp associated with the dominant liberal Labor Party of Norway killing 69 people, mostly teenagers. His act of mass murder captured world attention, as did his electronic distribution of an infamous document entitled 2083 – A European Declaration of Independence, which proclaims a right-wing world view with unyielding hostility towards...
Show moreOn July 22, 2011 Anders Behring Breivik bombed a government building in Oslo, resulting in the deaths of eight people. A few hours later he attacked a youth camp associated with the dominant liberal Labor Party of Norway killing 69 people, mostly teenagers. His act of mass murder captured world attention, as did his electronic distribution of an infamous document entitled 2083 – A European Declaration of Independence, which proclaims a right-wing world view with unyielding hostility towards multiculturalism and the alleged "Islamization of Europe. While right-wing extremist groups in Norway has been weak and insignificant over the past decades, the large populist right wing party, the Progress Party (Fremskrittspartiet), has sustained a central role in the liberal democracy with a critical position on immigrant issues. My research focuses on the underlying ideology of historical and contemporary right wing extremism in Norway. This includes, among others, the Norwegian Nobel Laureate and Nazi sympathizer Knut Hamsun, the Norwegian fascist party (Nasjonal Samling) and the contemporary presence of xenophobic, anti-immigration and anti-Islamic right wing in Norway, including the larger context of the ideology and behavior of the mass-murderer Breivik. My research, drawing on archival research on the extreme right in Scandinavia and Europe and interviews with several prominent psychiatrists, politicians, authors on Knut Hamsun and experts on radical-right in Norway, suggest that close parallels can be noted between the rhetoric of Nazi anti-Semitism and modern Islamophobia, with incidental differences of group identities and the basis for perceiving a threat. Within the various forms of right wing extremism there are strikingly similar ideological structures used to justify political violence, and Breivik is a textbook example of how the growing presence of the right-wing extremist activity online can only be ignored at our peril.
Show less - Date Issued
- 2013
- Identifier
- FSU_migr_uhm-0226
- Format
- Thesis
- Title
- Building Worlds: Creating an Affordable and Efficient Virtual Reality.
- Creator
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Kyle, Mari M., Department of Art
- Abstract/Description
-
The objective of Building Worlds; Creating an Affordable and Efficient Virtual Reality is to explore the phenomena of Virtual Reality (VR) through the marketing of its developing technologies, its future opportunities, and its potential of mass production. I spent the fall of 2014, gathering information on current VR and immersive environment trends. In the spring of 2015 I began analyzing the current market of VR and evaluating its advertising trends to explore the opportunities of VR in...
Show moreThe objective of Building Worlds; Creating an Affordable and Efficient Virtual Reality is to explore the phenomena of Virtual Reality (VR) through the marketing of its developing technologies, its future opportunities, and its potential of mass production. I spent the fall of 2014, gathering information on current VR and immersive environment trends. In the spring of 2015 I began analyzing the current market of VR and evaluating its advertising trends to explore the opportunities of VR in various fields such as education, art, psychology, and entertainment, etc. This thesis will offer a quick introduction to the history of VR and then proceed to analyze the market of VR during the '90s "Fall of VR" and compare it to the market of the present. I interviewed the nations VR pioneers and gathered from their discussions a set of strengths and weaknesses of current technologies. This thesis will combine this inside-industry perspective with an audience perspective (backed by market research) in order to study the present day market. Ultimately, these studies have revealed a change in target demographic and societal interest for VR. Through my industry studies, I can conclude also that VR applications in entertainment are the most influential and renowned in popular media, suggesting a change in current advertising strategy. This is supported by the conducted research that showed that the target demographic is unaware of the industrial uses of VR that have been extant for decades but very aware of the entertainment-based uses of VR.
Show less - Date Issued
- 2015
- Identifier
- FSU_migr_uhm-0500
- Format
- Thesis
- Title
- Business Continuity Risk Management in Light of Climate Change.
- Creator
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Storts, Monica
- Abstract/Description
-
This paper evaluates the importance of business preparation for climate change, how well organizations appear to be prepared for it, and how business entities may better protect their operations from the risks of climate change. As suggested by PREP (Partnership for Resilience and Environmental Preparedness program), companies need to assess the opportunities to bring climate change resilience into business value chains. Furthermore, businesses must determine the extent to which the risks...
Show moreThis paper evaluates the importance of business preparation for climate change, how well organizations appear to be prepared for it, and how business entities may better protect their operations from the risks of climate change. As suggested by PREP (Partnership for Resilience and Environmental Preparedness program), companies need to assess the opportunities to bring climate change resilience into business value chains. Furthermore, businesses must determine the extent to which the risks faced by its external stakeholders impact the business environment. This paper asserts it is a clear shift in business planning that is called for – from a focus on recovery from interruption to a focus on anti-interruption (actual continuity). Prior studies have suggested that the development and capability of staff to ensure that business continuity plans can be enacted in a timely and effective manner is an essential start (Paton, 2009). In order to develop effective climate change business continuity plans, businesses need to implement a culture of resilience and adaptation. Planning and preparedness are essential to a solid foundation for these types of cultures and continuity plans. Creative problem-solving approaches, which emphasize climate change as a social dilemma were pursued, and recommendations offered for businesses.
Show less - Date Issued
- 2017-04-24
- Identifier
- FSU_libsubv1_scholarship_submission_1493071013
- Format
- Thesis
- Title
- Can Conversation Partners Effectively Influence Others’ Perception of Them?.
- Creator
-
Simonin, Natalie
- Abstract/Description
-
The purpose of this study is to examine if people are successful in creating an impression when interacting with someone they just met. Participants were given ten minutes to either engage in conversation with a partner, or they were given a goal to make their partner view them as likeable or unlikeable. Both partners then answered a series of questions about each other. The results showed that participants were successful in meeting their goals. Big Five personality traits of both the...
Show moreThe purpose of this study is to examine if people are successful in creating an impression when interacting with someone they just met. Participants were given ten minutes to either engage in conversation with a partner, or they were given a goal to make their partner view them as likeable or unlikeable. Both partners then answered a series of questions about each other. The results showed that participants were successful in meeting their goals. Big Five personality traits of both the participant and their partner did not show a major impact on how effective the participants were in accomplishing their goals.
Show less - Date Issued
- 2018-04-23
- Identifier
- FSU_libsubv1_scholarship_submission_1524858077_941e2c68
- Format
- Thesis
- Title
- Can't Choose Your Family, and other stories.
- Creator
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Avagliano, Kathryn, Department of English
- Abstract/Description
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This work is a collection of short stories entitled Can't Choose Your Family. The collections consists of six stories produced during writing workshops at Florida State University. Humans have a great instinct for family, yet it's the people we live with day after day that can be the greatest mysteries. I was raised in a large family—mother and father, two sisters, one brother, a revolving door of dogs underfoot. My best friends are my siblings, and the greatest people I know are my parents,...
Show moreThis work is a collection of short stories entitled Can't Choose Your Family. The collections consists of six stories produced during writing workshops at Florida State University. Humans have a great instinct for family, yet it's the people we live with day after day that can be the greatest mysteries. I was raised in a large family—mother and father, two sisters, one brother, a revolving door of dogs underfoot. My best friends are my siblings, and the greatest people I know are my parents, and yet, even though stories are told every night at long dinners there were some mysteries that were never solved. My father was expelled from high school. My mother quit veterinary school to become a doctor. I see other taboo subjects in the families of my friends. A seemingly safe subject is mentioned and someone will laugh too fast and say, "Oh, we never talk about that." These unspeakable stories are the most interesting ones, and I was not surprised when my writing started to explore the nature of secrets within a family. The stories I have chosen to revise and include in Can't Choose Your Family all revolve around the various alliances and secrets that spring up within a family group, both blood relations and close family-like friends. The title piece takes place in a small town and tells the story of a teenage boy whose father is accused of touching another boy on his wrestling squad. In the story, I concentrated on the many things that remain unsaid between fathers and sons. Real Money explores a love triangle as a trio of friends run diamonds into New York City. In 21st Century Witchcraft, a newly engaged young woman is reminded of a life she's been successfully avoided when her nephew commits suicide. Growing Pains takes place in 1944 and revolves around a young boy who is left with his grandfather to coalesce after a long illness and spends the time wishing he was in the war. It is important for writers to "write what you know," which I believe I've done in writing about strong family bonds. Yet it is equally important to write about what we don't know, to probe the situations too tragic or strange to arise in our own lives, to explore different perspectives and situations and come out on the other side.
Show less - Date Issued
- 2014
- Identifier
- FSU_migr_uhm-0439
- Format
- Thesis
- Title
- Capillary Electrophoresis Separation of Formaldehyde Cross-Linked Aptamer-Protein Complexes.
- Creator
-
Duren, Joseph, Biochemistry
- Abstract/Description
-
An Immunoglobulin-E (IgE) DNA aptamer was covalently bound to IgE using a formaldehyde cross-linking method. The resulting complex was then separated from free aptamer by capillary electrophoresis (CE). The bound-to-free peak area ratios were preserved as a result of the cross-linking, which acted as a "freeze-frame" of the reaction in equilibrium. Elevated temperature occurring during CE separations can cause dissociation of non-covalently bound molecules and their targets, decreasing the...
Show moreAn Immunoglobulin-E (IgE) DNA aptamer was covalently bound to IgE using a formaldehyde cross-linking method. The resulting complex was then separated from free aptamer by capillary electrophoresis (CE). The bound-to-free peak area ratios were preserved as a result of the cross-linking, which acted as a "freeze-frame" of the reaction in equilibrium. Elevated temperature occurring during CE separations can cause dissociation of non-covalently bound molecules and their targets, decreasing the usefulness of the information obtained. Due to the formaldehyde cross-linking performed, the bound-to-free ratio was preserved over that of the uncross-linked control, which showed a decreased bound-to-free ratio upon heating. Additional trials were run on bovine serum albumin (BSA), anti-BSA, glucagon, and anti-glucagon using the formaldehyde cross-linking method. The results for this section were not conclusive due to difficulties in the separation and mechanistic issues with formaldehyde cross-linking. With the use of formaldehyde cross-linking, complexes that easily dissociate, under stringent separation conditions, can be covalently linked to prevent dissociation and allow for more sensitive analyses to be performed. Keywords:
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0012
- Format
- Thesis
- Title
- Carbon Nanotype Composite Sample Preparation by Using Microtome for TEM Analysis.
- Creator
-
Trayner, Sarah, Engineering - Industrial
- Abstract/Description
-
Transmission Electron Microscope, or TEM, is one of the most valuable tools used in materials science today. It is valuable because it allows the observer to study materials at an atomic level. However, TEM needs to use extremely thin samples (< 50 nm) for electron beam transparency. There are various methods such as crushing, ion beam thinning, ultramicrotomy, cryo-ultramicrotomy, etc. used to obtain an adequate sample for TEM analysis. But to produce quality TEM samples is very challenging...
Show moreTransmission Electron Microscope, or TEM, is one of the most valuable tools used in materials science today. It is valuable because it allows the observer to study materials at an atomic level. However, TEM needs to use extremely thin samples (< 50 nm) for electron beam transparency. There are various methods such as crushing, ion beam thinning, ultramicrotomy, cryo-ultramicrotomy, etc. used to obtain an adequate sample for TEM analysis. But to produce quality TEM samples is very challenging due to the huge characteristics differences of various materials. This research is an effort to study the effects of cutting parameters via ultra-microtome on TEM sample quality for carbon nanotube (CNT) composite samples. The CNT composite samples of focus are the nanotube yarn composites. Because nanotubes exhibit extreme strength and stiffness, appropriate cutting parameters should be chosen according to the material properties. During sample preparation, the nanotube yarn composite was embedded longitudinally in Armorstar resin and then cut using the microtome. The results showed that the parameters chosen did not lead to obtaining good TEM samples when comparing to the baseline TEM images. This is most likely due to the fact that the experimental sample is a nanotube yarn composite. Each experimental sample cutting block had three very small nanotube yarn composites embedded longitudinally in the Armorstar resin. When cutting the block using the ultra-microtome, only a small part of the nanotube yarn was cut compared to the overall sample surface area. As a result, obtaining a uniformly thin sample becomes challenging due to the cross section of the cutting block may have large property variations. Further study on the cutting block preparation and cutting parameters are needed.
Show less - Date Issued
- 2012
- Identifier
- FSU_migr_uhm-0051
- Format
- Thesis