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Title: Activity-Dependent Regulation of Calcium and Ribosomes in the Chick Cochlear Nucleus.
Creator: Call, Cody, Department of Psychology
Abstract/Description: Cochlea removal results in the death of 20-30% of neurons in nucleus magnocellularis (NM), a cochlear nucleus of the chick auditory system involved in the precise time-coding of acoustic signals. Within 1 hr of deafferentation, intracellular calcium concentration ([Ca2+]i) rises by up to 400% while the integrity of ribosomes begins to decline—two potentially cytotoxic events. Glutamatergic axons of the auditory nerve have been shown to maintain NM neuron health by activating group I and II...
Show moreCochlea removal results in the death of 20-30% of neurons in nucleus magnocellularis (NM), a cochlear nucleus of the chick auditory system involved in the precise time-coding of acoustic signals. Within 1 hr of deafferentation, intracellular calcium concentration ([Ca2+]i) rises by up to 400% while the integrity of ribosomes begins to decline—two potentially cytotoxic events. Glutamatergic axons of the auditory nerve have been shown to maintain NM neuron health by activating group I and II metabotropic glutamate receptors (mGluRs), maintaining normal [Ca2+]i and ribosomal integrity. This study aimed to determine how [Ca2+]i and ribosomal integrity are maintained by auditory nerve stimulation by selectively blocking group I mGluRs with AIDA and group II mGluRs with LY 341495 during unilateral auditory nerve stimulation. The abundance of Ca2+ in NM neurons was quantified using in vitro fura-2 ratiometric calcium imaging, while ribosomal integrity was assayed in a subset of the same tissue slices using Y10B immunolabeling (Y10B-ir). It was expected that AIDA and LY 341495 would increase [Ca2+]i and these increases would occur in parallel with an elimination in stimulation-induced differences in Y10B-ir between stimulated and unstimulated neurons of a slice. AIDA caused large increases in [Ca2+]i and eliminated differences in Y10B-ir between sides. Surprisingly, LY 341495 failed to cause reliable increases in [Ca2+]i compared to stimulated controls, but still eliminated differences in Y10B-ir between sides. These results suggest dissociation in how calcium and ribosomes are regulated in NM neurons.
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Date Issued: 2015
Identifier: FSU_migr_uhm-0509
Format: Thesis

Title: The Afferent Circuitry of the Ventromedial Hypothalamus and Its Activation in Paternal Behavior of the Socially Monogamous Prairie Vole.
Creator: Rogers, Richard S., Department of Chemistry and Biochemistry
Abstract/Description: Paternal behavior is an interesting and important research topic due to its integral contribution to the fitness and well-being of multiple species, including humans. Although paternal behavior is well described in literature, attempts at neurobiological characterization have yielded conflicting results that fail to address brain region interconnectivity. This study was designed to evaluate the relationship between afferent VMH circuitry and the onset of paternal behavior, using the prairie...
Show morePaternal behavior is an interesting and important research topic due to its integral contribution to the fitness and well-being of multiple species, including humans. Although paternal behavior is well described in literature, attempts at neurobiological characterization have yielded conflicting results that fail to address brain region interconnectivity. This study was designed to evaluate the relationship between afferent VMH circuitry and the onset of paternal behavior, using the prairie vole (Microtus ochrogaster) model. Sexually naïve male prairie voles received injections of the retrograde neurotracer Fluoro-Gold (FG), into the VMH. Two weeks later, subjects were exposed to either conspecific pups, contained within a tea-ball, or an empty tea-ball (control) for 1 hr. Immunohistochemical labeling was conducted for both FG and the neuronal activity marker Egr-1, in order to evaluate neuronal and afferent pathway activation between the ventromedial hypothalamus (VMH) and the amygdala (AMYG), bed nucleus of the stria terminalis (BNST), lateral septum (LS) and ventral tegmental area (VTA). Similar to the pathway implicated in the onset of maternal behavior, the results of this study showed pup exposure-induced neuronal activation in the AMYG and BNST, particularly in the efferent pathways from these two brain areas to the VMH. This effect was not found in the LS and VTA projection neurons to the VMH. Together, the data suggests a brain region-specific neuronal activation by pup exposure in particular brain circuitry, implicating its possible involvement in paternal behavior.
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Date Issued: 2015
Identifier: FSU_migr_uhm-0545
Format: Thesis

Title: Attention Deficit/Hyperactivity Disorder (ADHD) and Kv1.3: Evaluating a Potential Disease Model.
Creator: Hoffman, Carlie, Department of Biological Science
Abstract/Description: Attention deficit/hyperactivity disorder (ADHD) is a condition characterized by excessive levels of impulsivity, inattention, and hyperactivity. Mice subjected to deletion of the Kv1.3 potassium ion channel (knockout, KO) were observed to display behavioral symptoms of ADHD, indicating they may model this disorder. Due to the comorbidity between ADHD and anxiety disorders, the anxiety levels of KO and wildtype (WT) mice were determined as a precursor for the presence of additional ADHD-type...
Show moreAttention deficit/hyperactivity disorder (ADHD) is a condition characterized by excessive levels of impulsivity, inattention, and hyperactivity. Mice subjected to deletion of the Kv1.3 potassium ion channel (knockout, KO) were observed to display behavioral symptoms of ADHD, indicating they may model this disorder. Due to the comorbidity between ADHD and anxiety disorders, the anxiety levels of KO and wildtype (WT) mice were determined as a precursor for the presence of additional ADHD-type behaviors using marble burying, elevated plus maze, and light-dark box testing. Because ADHD affects children and adults alike, inattentive and hyperactive behaviors were quantified for young and aged mice of both genotypes. Methylphenidate (MPH) or saline were also administered to the mice via oral gavage to determine the influence of MPH on behavior. Anxiety testing indicated that KO mice trended towards having decreased anxiety levels compared to their WT counterparts. Object-based attention testing indicated young and aged KO mice had attention deficits and treatment with MPH ameliorated this symptom. However, metabolic chamber testing revealed that WT and KO mice had equivalent activity levels and MPH treatment had no influence on locomotor activity. Based on these findings, there is a link between enhanced olfactory ability and decreased anxiety, and KO mice may be used as behavioral models of the inattentive subtype of ADHD.
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Date Issued: 2014
Identifier: FSU_migr_uhm-0316
Format: Thesis

Title: Characterization of Autophagy within the Circadian Framework.
Creator: Rich, Natasha, Department of Biomedical Sciences
Abstract/Description: Previous studies suggest that the PER protein and its rhythmicity is the most critical component for clock functioning. This project expands on this premise to look at degradation methods used by mammals to degrade PER in the clock pathway. Our unpublished data suggest that the circadian protein PER is degraded by both the ubiquitin-proteasome system (in the nucleus) and autophagy (in the cytoplasm). Initial work included monitoring effects of starvation on induction of autophagy and PER...
Show morePrevious studies suggest that the PER protein and its rhythmicity is the most critical component for clock functioning. This project expands on this premise to look at degradation methods used by mammals to degrade PER in the clock pathway. Our unpublished data suggest that the circadian protein PER is degraded by both the ubiquitin-proteasome system (in the nucleus) and autophagy (in the cytoplasm). Initial work included monitoring effects of starvation on induction of autophagy and PER levels. Recently, by manipulating genes that directly control formation of proteins integral to autophagy function, we have come across a circadian behavioral phenotype. We plan to continue to investigate this phenotype through both in vivo and in vitro assays.
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Date Issued: 2015
Identifier: FSU_migr_uhm-0457
Format: Thesis

Title: The Drug Development and Business of Traumatic Brain Injury.
Creator: Kearney, Ryan, Department of Management
Abstract/Description: This thesis directly deals with the developmental path and business aspects behind bringing a drug to market. The drug and science that is focused on pertains to concussion and treatment of this condition. If you are a scientist developing a pharmaceutical drug or if you are a member of the development team, settle in because this is read for you. This story and insight will take you along the path for the development and formation of a company around a concussion-treating drug. This story...
Show moreThis thesis directly deals with the developmental path and business aspects behind bringing a drug to market. The drug and science that is focused on pertains to concussion and treatment of this condition. If you are a scientist developing a pharmaceutical drug or if you are a member of the development team, settle in because this is read for you. This story and insight will take you along the path for the development and formation of a company around a concussion-treating drug. This story covers what the drug is, how it was developed, the pitfalls and successes that were navigated, and where the Prevacus team resides today. It has excerpts from other success stories and is essentially a bible for your own drug development and how to get a drug passed by the FDA. While this is a true story of the success and improvement of treatment for a leading health condition, there are many areas that could have been navigated differently if you are planning to take yourself down a similar path.
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Date Issued: 2014
Identifier: FSU_migr_uhm-0323
Format: Thesis

Title: The Effect of Physical and Emotional Stress on Nicotine Consumption in Male Mice.
Creator: Sattler, Carley, Department of Psychology
Abstract/Description: This experiment was designed to examine the impact of chronic stress exposure on nicotine consumption. Adolescent (postnatal days, PD 38-75) male mice underwent 10 days of social defeat, were treated with one month (long-term) of forced nicotine consumption (160 mg/l), and their reactivity to various behavioral paradigms (social interaction test (SIT), elevated plus maze (EPM), and forced swim test (FST)) was assessed. Findings indicate that chronic nicotine exposure following social defeat...
Show moreThis experiment was designed to examine the impact of chronic stress exposure on nicotine consumption. Adolescent (postnatal days, PD 38-75) male mice underwent 10 days of social defeat, were treated with one month (long-term) of forced nicotine consumption (160 mg/l), and their reactivity to various behavioral paradigms (social interaction test (SIT), elevated plus maze (EPM), and forced swim test (FST)) was assessed. Findings indicate that chronic nicotine exposure following social defeat may effectively buffer against some anxiety- and depression-like symptoms later in life.
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Date Issued: 2014
Identifier: FSU_migr_uhm-0345
Format: Thesis

Title: The Effects of a Hyperlipidermic Diet on Olfactory Sensory Neuron Expression of the Immediate Early Gene c-fos in Mice.
Creator: Cooper, Melissa, Department of Biological Science
Date Issued: 2014
Identifier: FSU_migr_uhm-0298
Format: Thesis

Title: The Effects of Mating Experience on Motivation and Anxiety-like Behaviors in Adult Male Rats.
Creator: Vitale, Erika, Program in Neuroscience
Abstract/Description: Social and physical aspects of sexual intercourse provide protective benefits on mental and physical health in humans. Similarly, in rodents, mating promotes resistance to anxiety- and- depressive-like behaviors. Furthermore, our lab has shown that sexually experienced males are also more resistant to the effects of stress, as evidenced by decreased neuronal stress-reactivity in the paraventricular nucleus of the hypothalamus. However, little is known about the possible effects of recent...
Show moreSocial and physical aspects of sexual intercourse provide protective benefits on mental and physical health in humans. Similarly, in rodents, mating promotes resistance to anxiety- and- depressive-like behaviors. Furthermore, our lab has shown that sexually experienced males are also more resistant to the effects of stress, as evidenced by decreased neuronal stress-reactivity in the paraventricular nucleus of the hypothalamus. However, little is known about the possible effects of recent mating or mating abstinence on motivation for other natural rewards. Here, we tested whether mating history, at different time periods, would affect measures of motivation and/or anxiety-like behaviors. We hypothesized that recent sexual activity would protect against anxiety-like behavior and anhedonia, whereas loss of sexual activity would have the opposite effect, compared to control males. The study found that recent mating increased motivation for a food reward in an anxiety-provoking novel setting, while abstinence from sexual experience decreased sucrose consumption at lower sucrose concentrations.
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Date Issued: 2013
Identifier: FSU_migr_uhm-0157
Format: Thesis

Title: The Effects of Paternal Deprivation on the Social and Anxiety-like Behavior in Male and Female Prairie Voles.
Creator: Phelan, Shana, Department of Psychology
Abstract/Description: Varies studies done on humans and animal models alike show the importance of healthy social relationships. Parental care (especially bi-parental care) is shown to be an important factor for the proper development and overall outcome of an individual. In this study, the prairie vole will be used to examine the effects of paternal deprivation on the social and anxiety-like behaviors of the F1 generation. Four behavioral tests were conducted to determine the effects of father deprivation on...
Show moreVaries studies done on humans and animal models alike show the importance of healthy social relationships. Parental care (especially bi-parental care) is shown to be an important factor for the proper development and overall outcome of an individual. In this study, the prairie vole will be used to examine the effects of paternal deprivation on the social and anxiety-like behaviors of the F1 generation. Four behavioral tests were conducted to determine the effects of father deprivation on parental behaviors, anxiety-like behaviors, social affiliation, and social recognition.
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Date Issued: 2015
Identifier: FSU_migr_uhm-0486
Format: Thesis

Title: The Effects of Testosterone on zif268 Expression and Its Potential Role in Learning and Memory.
Creator: Bunn, Corinne, Department of Biological Science
Date Issued: 2013
Identifier: FSU_migr_uhm-0185
Format: Thesis

Title: Interactions Between Vasoactive Intestinal Polypeptide and Dopaminergic Neurons in the Mammalian Olfactory Bulb.
Creator: Herman, Allan T., Trombley, Paul Q., Korshunov, Kirill, Department of Biological Science
Abstract/Description: In the olfactory bulb (OB), vasoactive intestinal polypeptide (VIP) neurons have a role in modulation of the interneuronal network. It has been known to be expressed in the granule cell layer (GCL), external plexiform layer (EPL), and the glomerular layer (GL). One of the functions of VIP in the OB is maintaining olfactory circadian rhythms (Miller et al., 2014). In the OB, dopaminergic (DA) neurons help process olfactory information through the release of dopamine (DA) onto the presynaptic...
Show moreIn the olfactory bulb (OB), vasoactive intestinal polypeptide (VIP) neurons have a role in modulation of the interneuronal network. It has been known to be expressed in the granule cell layer (GCL), external plexiform layer (EPL), and the glomerular layer (GL). One of the functions of VIP in the OB is maintaining olfactory circadian rhythms (Miller et al., 2014). In the OB, dopaminergic (DA) neurons help process olfactory information through the release of dopamine (DA) onto the presynaptic terminals of olfactory sensory neurons and the dendrites of mitral and tufted (M/T) cells, leading to inhibition of transmitter release (Berkowicz and Trombley, 2000; Davila et al., 2003; Ennis et al., 2001). Tyrosine hydroxylase (TH), the rate-limiting enzyme in the metabolic production of DA, is present in all DA neurons and is used as a histological marker. TH is involved in producing L-DOPA from L-Tyrosine which then enables the production of DA. DA neurons appear to be restricted to a sub-population of neurons within the GL (see Nagayama et al., 2014 for review). The distribution of VIP is less clear and appears to vary among mammalian species. The aim of this project is to determine whether VIP and/or VIP receptors (VPAC2) co-localize with DA in the GL using a combination of immunohistochemistry and fluorescence microscopy. Although the data for VIP remain inconclusive, some cells may show co-labeling of both VPAC2 and TH in the GL suggesting that glomerular DA neurons may be a target of VIP.
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Date Issued: 2015
Identifier: FSU_migr_uhm-0488
Format: Thesis

Title: A Multipurpose Protocol to Characterize Concussion Symptoms in College Athletes.
Creator: Galletti, Christopher, Department of Biological Science
Abstract/Description: With 1.6 to 3.8 million sports-related concussions (SRCs) per year and an uncertain definition of concussion or mild traumatic brain injury (mTBI), there is a great need for a model to clarify the diagnosis and assessment of concussions and mTBIs. In addition, the effects of post-concussion syndrome (PCS) can be debilitating if not properly treated. College athletes are at an increased risk for repetitive head injury while still suffering from PCS, which can lead to the rare and deadly second...
Show moreWith 1.6 to 3.8 million sports-related concussions (SRCs) per year and an uncertain definition of concussion or mild traumatic brain injury (mTBI), there is a great need for a model to clarify the diagnosis and assessment of concussions and mTBIs. In addition, the effects of post-concussion syndrome (PCS) can be debilitating if not properly treated. College athletes are at an increased risk for repetitive head injury while still suffering from PCS, which can lead to the rare and deadly second impact syndrome (SIS). To improve the management and assessment of concussions, a comprehensive approach will provide objective and quantitative measures using novel methods including postural stability and aerobic exercise testing to complement existing measures of standard assessment of concussion (SAC), concussion grading scale (CGS), and ImPACT. This pilot study collected baseline data from subjects using this multipurpose protocol, which will serve as a reference when comparing concussed subjects. This study provides the framework to diagnose and manage the treatment of concussed college athletes in order to provide an accelerated, yet safe return-to-play (RTP).
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Date Issued: 2014
Identifier: FSU_migr_uhm-0309
Format: Thesis

Title: Neuronal Health Following Diet-Induced Obesity at Middle Age in Mice as Determined by Immediate Early Gene Markers.
Creator: Celen, Arda B., Department of Biological Science
Abstract/Description: Diet-induced obesity (DIO) is a growing health concern in the United States, with more than a third of the population suffering from this disease. Deficits in many sensory processes such as olfaction, audition and vision have been linked to DIO. Electro-olfactogram tests show that DIO results in reduction of electrical response to odors, indicating a functional decrease in olfaction. My research investigates how DIO affects the activation of an odor-specific population of periglomerular (PG)...
Show moreDiet-induced obesity (DIO) is a growing health concern in the United States, with more than a third of the population suffering from this disease. Deficits in many sensory processes such as olfaction, audition and vision have been linked to DIO. Electro-olfactogram tests show that DIO results in reduction of electrical response to odors, indicating a functional decrease in olfaction. My research investigates how DIO affects the activation of an odor-specific population of periglomerular (PG) neurons surrounding the M72 glomeruli, using the immediate early gene c-fos as an activity marker. Using mice that had been kept on dietary regimes of control fat (CF), moderately-high fat (MHF), high fat (HF), and CF switched to MHF at middle age (CF-MHF), I started to assess the c-fos signaling in their M72 PG cells after placing the animals in an odor stimulation apparatus and administering the odorant isopropyl tiglate. The weight measurements showed that experimental mice weighed significantly higher than their CF counterparts. An intraperitoneal glucose tolerance test (IPGTT) was performed to confirm that experimental mice were pre-diabetic, as evidenced by their inefficiency in clearing injected glucose from their blood. At this stage, I am investigating how high fat diet alters neuronal activity by performing cell counts for c-fos positive PG cells and assessing whether there are any modifications to neural coding as indicated by spatial activation patterns. By documenting the effects of poor diet on brain function, my lab is aiming to raise public awareness and lay the groundwork for future medical research.
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Date Issued: 2015
Identifier: FSU_migr_uhm-0494
Format: Thesis

Title: Neurotransmitter Interactions that Control High-Fat Food Intake.
Creator: Gooldy, Timothy, Department of Nutrition, Food, and Exercise Science
Abstract/Description: Overconsumption of highly palatable "junk food" contributes to obesity and related metabolic disorders. This project investigated the brain circuitry that allows such eating in excess of the body's energy requirements. We hypothesized that neurotransmitters mediating energy homeostasis and food reward interact within the nucleus accumbens (NAc) to control the intake of palatable foods. Specifically, we examined two neuronal projections from the hindbrain to the NAc: glucagon-like peptide 1 ...
Show moreOverconsumption of highly palatable "junk food" contributes to obesity and related metabolic disorders. This project investigated the brain circuitry that allows such eating in excess of the body's energy requirements. We hypothesized that neurotransmitters mediating energy homeostasis and food reward interact within the nucleus accumbens (NAc) to control the intake of palatable foods. Specifically, we examined two neuronal projections from the hindbrain to the NAc: glucagon-like peptide 1 (GLP-1) and the A2 population of noradrenergic (NA) neurons. Both GLP-1 and A2 cell bodies are located in the nucleus of the solitary tract (NTS) and receive input from the gastrointestinal tract about incoming nutrients during meals. Our lab has previously shown that GLP-1 can act within the NAc to reduce feeding, but that maintenance on high-fat diet (HFD) impairs this response. We hypothesized that this impairment is caused by endogenous opioid activation of mu-opioid receptors (MOR) in NAc. Our studies further examined the HFD-induced impairment in GLP-1 sensitivity and attempted to lay the groundwork for investigating a possible interaction between GLP-1 and MOR in NAc. The next series of studies focused on the role of the A2 projection to NAc. We had previously found that selective lesion of this projection caused overeating and weight gain. Here we asked whether this lesion affects energy expenditure or locomotor activity. We did not observe any significant effects, but also failed to replicate the body weight effect previously obtained, so the data are not conclusive. Further analysis is ongoing.
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Date Issued: 2012
Identifier: FSU_migr_uhm-0144
Format: Thesis

Title: The Role of Orosensory and Post-Ingestive Feedback in Salivary Protein Production.
Creator: Villalobos, Maria, Department of Psychology
Abstract/Description: Variation in bitter taste perception plays a crucial role in dietary choice and much research has been done to better understand the factors that cause variation in bitter taste perception. One factor that may cause variation in bitter taste perception is saliva. Salivary protein expression can be altered by diet [11]. For example, while we know that tannin diets cause the upregulation of proline-rich proteins (PRPs) [11], it is still unclear if it is oral exposure, gastric exposure or tannin...
Show moreVariation in bitter taste perception plays a crucial role in dietary choice and much research has been done to better understand the factors that cause variation in bitter taste perception. One factor that may cause variation in bitter taste perception is saliva. Salivary protein expression can be altered by diet [11]. For example, while we know that tannin diets cause the upregulation of proline-rich proteins (PRPs) [11], it is still unclear if it is oral exposure, gastric exposure or tannin exposure at both sites that is responsible for the upregulation of PRPs. In this study we were able to better understand how salivary proteins are induced by analyzing the saliva of rats treated with oral exposure alone (via oral infusion), gastric exposure alone (via gastric infusion), or exposure at both sites with a tannic acid solution. Our preliminary analyses demonstrate a subset of proteins that are upregulated by dietary exposure are upregulated by oral exposure alone (35kDa, 25kDa and 19kDa) demonstrating that for these proteins oral exposure is sufficient. Furthermore, as these proteins are not upregulated by gastric exposure, we believe oral exposure is necessary for upregulation to occur. In contrast, for a protein band at 18kDa, oral exposure did not effect protein expression while gastric exposure alone was sufficient and necessary in order for upregulation to occur.
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Date Issued: 2014
Identifier: FSU_migr_uhm-0355
Format: Thesis

Title: Serotonin Manipulation Affects Behaviors in Male Prairie Voles.
Creator: Diaz, Devon, Department of Biological Science
Abstract/Description: The purpose of the current study was to investigate whether chronic manipulation of 5-HT by fluoxetine administration alters the social and anxiety-like behaviors in male prairie voles. Adult prairie voles were treated with a high dose 6.0 mg/kg of fluoxetine, a low dose 0.6mg/kg of fluoxetine and saline as a control for 14 days and then subjected to many behavior tests including: the Open Field Test (OF), Elevated Plus Maze (EPM), Social Affiliation test, 6 Hour Cohabitation, Partner...
Show moreThe purpose of the current study was to investigate whether chronic manipulation of 5-HT by fluoxetine administration alters the social and anxiety-like behaviors in male prairie voles. Adult prairie voles were treated with a high dose 6.0 mg/kg of fluoxetine, a low dose 0.6mg/kg of fluoxetine and saline as a control for 14 days and then subjected to many behavior tests including: the Open Field Test (OF), Elevated Plus Maze (EPM), Social Affiliation test, 6 Hour Cohabitation, Partner Preference Test and Resident Intruder test. The results indicated that fluoxetine does have an effect on social behavior in three main areas: reduced anxiety leading to decrease in open arm latency of the EPM, increased social affiliation during the partner preference test following the 6 hour cohabitation and reduced anxiety leading to a more inquisitive nature show in the decrease of aggressive behavior in the Resident intruder test.
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Date Issued: 2013
Identifier: FSU_migr_uhm-0241
Format: Thesis

Title: Sex Differences in Reward and Behavioral Sensitization to Ketamine in Rats.
Creator: Schoepfer, Kristin, Department of Psychology
Abstract/Description: Major depressive disorder is a serious public mental health concern with prevalent sex differences, affecting twice as many women as men. The non-competitive NMDA receptor antagonist ketamine is being used clinically at low doses as an antidepressant for treatment-resistant patients, yielding rapid and robust therapeutic effects lasting up to one week. However, it is likely that long-term maintenance of ketamine's antidepressant effects will require repeated administration in many patients....
Show moreMajor depressive disorder is a serious public mental health concern with prevalent sex differences, affecting twice as many women as men. The non-competitive NMDA receptor antagonist ketamine is being used clinically at low doses as an antidepressant for treatment-resistant patients, yielding rapid and robust therapeutic effects lasting up to one week. However, it is likely that long-term maintenance of ketamine's antidepressant effects will require repeated administration in many patients. We have recently shown that female rats have a heightened sensitivity to ketamine's antidepressant effects that is mediated by gonadal hormones. Importantly, ketamine is also recreationally abused and is a Schedule III drug in the U.S. Determining the consequences and sex differences therein of repeated sub-anesthetic ketamine dosing is vital to the viability of the drug as a clinical antidepressant with regard to its pharmacologically rewarding and addictive properties. In this study, using the conditioned place preference, we assessed rewarding properties of several doses of ketamine (0, 2.5, 5.0, or 10 mg/kg i.p.) in adult male and female Sprague-Dawley rats. Additionally, animals' locomotor activity was monitored upon the first and sixth ketamine injections to assess behavioral sensitization to the locomotor activating effects of the drug. Our results show that male rats developed a place preference for only the 10 mg/kg ketamine dose. In contrast, females did not develop a place preference to any dose of ketamine. Both males and females displayed a significant increase in locomotor activity between the first and sixth administration of 5.0 and 10 mg/kg ketamine, which suggests that they developed a behavioral sensitization to these doses. These findings suggest that repeated treatment with ketamine induces dose- and sex-dependent effects on reward state and behavioral sensitization in male and female rats.
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Date Issued: 2014
Identifier: FSU_migr_uhm-0378
Format: Thesis

Neurobiology (12)	+ -
Brain chemistry (5)	+ -
Neurochemistry (5)	+ -
Biology (4)	+ -
Psychobiology (4)	+ -

Human behavior (3)	+ -
Behavior (2)	+ -
Drug addiction (2)	+ -
Neuropsychology (2)	+ -
Substance abuse (2)	+ -
Behavioral assessment (1)	+ -
Biochemistry (1)	+ -
Dairying (1)	+ -
Design (1)	+ -
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Entrepreneurship (1)	+ -
Finance (1)	+ -
Industrial management (1)	+ -
Management (1)	+ -
Medical economics (1)	+ -
Pharmaceutical chemistry (1)	+ -
Pharmaceutical policy (1)	+ -
Pharmacology (1)	+ -
Pharmacy management (1)	+ -
Psychology (1)	+ -
Small business (1)	+ -
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Department of Psychology (5)	+ -
Bunn, Corinne (1)	+ -
Call, Cody (1)	+ -
Celen, Arda B. (1)	+ -

Cooper, Melissa (1)	+ -
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Department of Management (1)	+ -
Department of Nutrition, Food, and Exercise Science (1)	+ -
Diaz, Devon (1)	+ -
Galletti, Christopher (1)	+ -
Gooldy, Timothy (1)	+ -
Herman, Allan T. (1)	+ -
Hoffman, Carlie (1)	+ -
Kearney, Ryan (1)	+ -
Korshunov, Kirill (1)	+ -
Phelan, Shana (1)	+ -
Program in Neuroscience (1)	+ -
Rich, Natasha (1)	+ -
Rogers, Richard S. (1)	+ -
Sattler, Carley (1)	+ -
Schoepfer, Kristin (1)	+ -
Trombley, Paul Q. (1)	+ -
Villalobos, Maria (1)	+ -
Vitale, Erika (1)	+ -

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