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X-ray Structure and Biophysical Properties of Rabbit Fibroblast Growth Factor 1

Title: X-ray Structure and Biophysical Properties of Rabbit Fibroblast Growth Factor 1.
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Name(s): Lee, Jihun, 1979-, author
Blaber, Sachiko, author
Irsigler, Andre, author
Aspinwall, Eric, author
Blaber, Michael, author
Type of Resource: text
Genre: text
Issuance: serial
Date Issued: 2009
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The rabbit is an important and de facto animal model in the study of ischemic disease and angiogenic therapy. Additionally, fibroblast growth factor 1 (FGF-1) is emerging as one of the most important growth factors for novel proangiogenic and pro-arteriogenic therapy. However, despite its significance, the fundamental biophysical properties of rabbit FGF-1, including its X-ray structure, have never been reported. Here, the cloning, crystallization, X-ray structure and determination of the biophysical properties of rabbit FGF-1 are described. The X-ray structure shows that the amino-acid differences between human and rabbit FGF-1 are solvent-exposed and therefore potentially immunogenic, while the biophysical studies identify differences in thermostability and receptor-binding affinity that distinguish rabbit FGF-1 from human FGF-1.
Identifier: FSU_migr_biomed_faculty_publications-0016 (IID)
Keywords: fibroblast growth factor, angiogenesis, FGF-1, rabbit, ischemia
Uncontrolled subjects: Amino Acid Sequence, Animals, Crystallography, X-Ray, Female, Fibroblast Growth Factor 1, Humans, Models, Molecular, Molecular Sequence Data, Protein Structure, Secondary, Protein Structure, Tertiary, Rabbits, Sequence Alignment, Sequence Homology, Amino Acid, Thermodynamics
Note: Originally published in Acta Crystallographica. Section F, Structural Biology and Crystallization Communications.
Citation: Lee, J., Blaber, S.I., Irsigler, A., Aspinwall, E. and Blaber, M. (2009). X-ray structure and biophysical properties of rabbit fibroblast growth factor-1. Acta Cryst. F. 65, 1097-1104.
Subject(s): Biochemistry
Biophysics
Molecular biology
Cytology
Developmental biology
Clinical biochemistry
Medical sciences
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_biomed_faculty_publications-0016
Owner Institution: FSU
Is Part of Series: Department of Biomedical Sciences Faculty Publications.
Is Part Of: Acta Crystallographica. Section F, Structural Biology and Crystallization Communications.
Issue: Pt 11, 65