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Experimental Support for the Evolution of Symmetric Protein Architecture from a Simple Peptide Motif

Title: Experimental Support for the Evolution of Symmetric Protein Architecture from a Simple Peptide Motif.
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Name(s): Lee, Jihun, 1979-, author
Blaber, Michael, author
Type of Resource: text
Genre: text
Issuance: serial
Date Issued: 2011
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The majority of protein architectures exhibit elements of structural symmetry, and "gene duplication and fusion" is the evolutionary mechanism generally hypothesized to be responsible for their emergence from simple peptide motifs. Despite the central importance of the gene duplication and fusion hypothesis, experimental support for a plausible evolutionary pathway for a specific protein architecture has yet to be effectively demonstrated. To address this question, a unique "top-down symmetric deconstruction" strategy was utilized to successfully identify a simple peptide motif capable of recapitulating, via gene duplication and fusion processes, a symmetric protein architecture (the threefold symmetric β-trefoil fold). The folding properties of intermediary forms in this deconstruction agree precisely with a previously proposed "conserved architecture" model for symmetric protein evolution. Furthermore, a route through foldable sequence-space between the simple peptide motif and extant protein fold is demonstrated. These results provide compelling experimental support for a plausible evolutionary pathway of symmetric protein architecture via gene duplication and fusion processes.
Identifier: FSU_migr_biomed_faculty_publications-0020 (IID)
Keywords: protein design, protein folding, protein symmetry
Uncontrolled subjects: Amino Acid Motifs, Calorimetry, Differential Scanning, Chromatography, Gel, Crystallography, X-Ray, Evolution, Molecular, Gene Duplication, Models, Genetic, Models, Molecular, Molecular Sequence Data, Mutagenesis, Peptides, Protein Conformation, Protein Folding, Ultracentrifugation
Note: Originally published in Proceedings of the National Academy of Sciences of the United States of America.
Citation: Lee, J. and Blaber, M. (2011). Experimental support for the evolution of symmetric protein architecture from a simple peptide motif. Proc. Natl. Acad. Sci. 108, 126-130.
Subject(s): Biochemistry
Biophysics
Molecular biology
Cytology
Developmental biology
Clinical biochemistry
Medical sciences
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_migr_biomed_faculty_publications-0020
Owner Institution: FSU
Is Part of Series: Department of Biomedical Sciences Faculty Publications.
Is Part Of: Proceedings of the National Academy of Sciences of the United States of America.
Issue: 1, 108

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Lee, J., & Blaber, M. (2011). Experimental Support for the Evolution of Symmetric Protein Architecture from a Simple Peptide Motif. Proceedings Of The National Academy Of Sciences Of The United States Of America. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_biomed_faculty_publications-0020