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Accelerated healing in NONcNZO10/LtJ type 2 diabetic mice by FGF 1

Title: Accelerated healing in NONcNZO10/LtJ type 2 diabetic mice by FGF 1 .
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Name(s): Blaber, Sachiko, author
Diaz, Jose, author
Blaber, Michael, author
Type of Resource: text
Genre: text
Date Issued: 2015-06-19
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The development of novel therapies to treat chronic diabetic ulcers depends upon appropriate animal models for early stage investigation. The NONcNZO10/LtJ mouse is a new polygenic strain developed to more realistically model human metabolic syndrome and obesity-induced Type 2 diabetes; however, detailed wound healing properties have not been reported. In this report we describe a quantitative wound healing study in the NONcNZO10/LtJ mouse using a splinted excisional wound. The rate of wound healing is compared to various controls, and is also quantified in response to topical administration of normal and mutant fibroblast growth factor-1 (FGF-1). Quantitation of re-epithelialization shows that the diabetic condition in the NONcNZO10/LtJ mouse is concomitant with a decreased rate of dermal healing. Furthermore, topical administration of a FGF-1/heparin formulation effectively accelerates re-epithelialization. A similar acceleration can also be achieved by a stabilized mutant form of FGF-1 formulated in the absence of heparin. Such accelerated rates of healing are not associated with any abnormal histology in the healed wounds. The results identify the NONcNZO10/LtJ mouse as a useful model of impaired wound healing in type II diabetes, and further, identify engineered forms of FGF-1 as a potential “second-generation” therapeutic to promote diabetic dermal wound healing.
Identifier: FSU_libsubv1_scholarship_submission_1456505007 (IID), 10.1111/wrr.12305 (DOI)
Keywords: Protein engineering, growth factor, diabetic ulcer, protein stability, fibroblast growth factor-1
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1456505007
Owner Institution: FSU
Is Part Of: Wound Repair and Regeneration.
Issue: iss. 4, vol. 23

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Blaber, S., Diaz, J., & Blaber, M. (2015). Accelerated healing in NONcNZO10/LtJ type 2 diabetic mice by FGF 1 . Wound Repair And Regeneration. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1456505007