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Evolution and design of protein structure by folding nucleus symmetric expansion

Title: Evolution and design of protein structure by folding nucleus symmetric expansion.
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Name(s): Longo, Liam, author
Kumru, Ozan, author
Middaugh, Russell, author
Blaber, Michael, author
Type of Resource: text
Genre: Text
Date Issued: 2014-10-07
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Models of symmetric protein evolution typically invoke gene duplication and fusion events, in which repetition of a structural motif generates foldable, stable symmetric protein architecture. Success of such evolutionary processes suggests that the duplicated structural motif must be capable of nucleating protein folding. If correct, symmetric expansion of a folding nucleus sequence derived from an extant symmetric fold may be an elegant and computationally tractable solution to de novo protein design. We report the efficient de novo design of a β-trefoil protein by symmetric expansion of a β-trefoil folding nucleus, previously identified by ɸ-value analysis. The resulting protein, having exact sequence symmetry, exhibits superior folding properties compared to its naturally evolved progenitor--with the potential for redundant folding nuclei. In principle, folding nucleus symmetric expansion can be applied to any given symmetric protein fold (that is, nearly 1/3 of the known proteome) provided information of the folding nucleus is available.
Identifier: FSU_libsubv1_scholarship_submission_1456503265 (IID), 10.1016/j.str.2014.08.008 (DOI)
Keywords: protein folding, protein design, protein evolution, folding nucleus, protein symmetry
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1456503265
Owner Institution: FSU
Is Part Of: Structure.
Issue: iss. 10, vol. 22

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Longo, L., Kumru, O., Middaugh, R., & Blaber, M. (2014). Evolution and design of protein structure by folding nucleus symmetric expansion. Structure. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1456503265