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Kallikrein-related Peptidase 6

Title: Kallikrein-related Peptidase 6: A Biomarker for Traumatic Brain Injury.
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Name(s): Phipps, Helen, author
Longo, Liam, author
Blaber, Sachiko, author
Blaber, Michael, author
VanLandingham, Jacob, author
Type of Resource: text
Genre: text
Date Issued: 2013-07-06
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Establishment of a traumatic brain injury (TBI)-sensitive biomarker or identification of a key therapeutic agent would significantly improve clinicians’ efforts to diagnose and treat TBI, thereby promoting improved outcomes for patients. Numerous studies support the role of kallikrein-6 (Klk6) as a critical component of neuroinflammation and demyelination. This study assesses whether Klk6 is implicated in the secondary mechanisms of TBI and subsequently if serum levels of Klk6 are useable as a biomarker. Methods: The abundance of Klk6 following controlled cortical impact (CCI) of the medial prefrontal cortex to a depth of either 3.0 mm (severe) or 1.5 mm (moderate) was quantified. Uninjured and rats subjected to craniotomy-only were used as controls. Protein levels were quantified with Western-blotting, enzyme-linked immunosorbent assay and immunohistochemistry. Results: Severe and moderate CCI resulted in significant elevation of Klk6 in the contusion-core (12-fold-increase, p 5 0.0001) and serum (5-fold-increase, p 5 0.01) compared to controls. In all cases, Klk6 elevation was resolved within 72 hours. Conclusion: Serum levels of Klk6 are a statistically significant indicator of TBI 24 hours after CCI and thus may be of great utility to clinicians as a biomarker. These data strongly implicate Klk6 as a player in the neuroinflammation processes following CCI, although the specific mechanisms remain to be characterized.
Identifier: FSU_libsubv1_scholarship_submission_1464402742 (IID), 10.3109/02699052.2013.823563 (DOI)
Keywords: Brain Injury
Publication Note: Oublisher's Version available at http://www.tandfonline.com/doi/abs/10.3109/02699052.2013.823563
Preferred Citation: Phipps, H. Longo, L. Blaber, S. Blaber, M. VanLandingham, J. (2006) Kallikrein-related peptidase 6: A biomarker for traumatic brain injury in the rat. Brain Injury. 27(13-14)
Grant Number: Tallahassee Memorial Hospital Bryan Robinson Endowment
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1464402742
Owner Institution: FSU
Is Part Of: Brain Injury.
Issue: iss. 13-14, vol. 27