You are here

Crystal Structure and Biochemical Characterization of Human Kallikrein 6 Reveals a Trypsin-like Kallikrein is Expressed in the Central Nervous System

Title: Crystal Structure and Biochemical Characterization of Human Kallikrein 6 Reveals a Trypsin-like Kallikrein is Expressed in the Central Nervous System.
29 views
13 downloads
Name(s): Bernett, Matthew, author
Blaber, Sachiko, author
Scarisbrick, Isobel, author
Dhanarajan, Pushparani, author
Thompson, Steven, author
Blaber, Michael, author
Type of Resource: text
Genre: text
Date Issued: 2002-04-30
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The human kallikreins are a large multigene family of closely related serine-type proteases. In this regard, they are similar to the multigene kallikrein families characterized in mice and rats. There is a much more extensive body of knowledge regarding the function of mouse and rat kallikreins in comparison with the human kallikreins. Human kallikrein 6 has been proposed as the homologue to rat myelencephalon-specific protease, an arginine-specific degradative-type protease abundantly expressed in the central nervous system and implicated in demyelinating disease. We present the x-ray crystal structure of mature, active recombinant human kallikrein 6 at 1.75-Å resolution. This high resolution model provides the first three-dimensional view of one of the human kallikreins and one of only a few structures of serine proteases predominantly expressed in the central nervous system. Enzymatic data are presented that support the identification of human kallikrein 6 as the functional homologue of rat myelencephalon-specific protease and are corroborated by a molecular phylogenetic analysis. Furthermore, the x-ray data provide support for the characterization of human kallikrein 6 as a degradative protease with structural features more similar to trypsin than the regulatory kallikreins.
Identifier: FSU_libsubv1_scholarship_submission_1464374012 (IID), 10.1074/jbc.M202392200 (DOI)
Publication Note: Publishers Version also available at http://www.jbc.org/content/277/27/24562
Preferred Citation: Bernett, M. Blaber, S. Blaber, M. Scarisbrick, I. Dhanarajan, P. Thompson, S. (2002). Crystal Structure and Biochemical Characterization of Human Kallikrein 6 Reveals That a Trypsin-like Kallikrein Is Expressed in the Central Nervous System. The Journal of Biological Chemistry. 277 (27). 24562-24570
Grant Number: National Multiple Sclerosis Society PP0757
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1464374012
Owner Institution: FSU
Is Part Of: Journal of Biological Chemistry.
Issue: iss. 27, vol. 277