You are here

X-ray structure and biophysical properties of rabbit fibroblast growth factor 1.

Title: X-ray structure and biophysical properties of rabbit fibroblast growth factor 1.
1 views
0 downloads
Name(s): Lee, Jihun, author
Blaber, Sachiko I, author
Irsigler, Andre, author
Aspinwall, Eric, author
Blaber, Michael, author
Type of Resource: text
Genre: journal article
text
Date Issued: 2009-11-01
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The rabbit is an important and de facto animal model in the study of ischemic disease and angiogenic therapy. Additionally, fibroblast growth factor 1 (FGF-1) is emerging as one of the most important growth factors for novel proangiogenic and pro-arteriogenic therapy. However, despite its significance, the fundamental biophysical properties of rabbit FGF-1, including its X-ray structure, have never been reported. Here, the cloning, crystallization, X-ray structure and determination of the biophysical properties of rabbit FGF-1 are described. The X-ray structure shows that the amino-acid differences between human and rabbit FGF-1 are solvent-exposed and therefore potentially immunogenic, while the biophysical studies identify differences in thermostability and receptor-binding affinity that distinguish rabbit FGF-1 from human FGF-1.
Identifier: FSU_pmch_19923726 (IID), 10.1107/S1744309109040287 (DOI), PMC2777034 (PMCID), 19923726 (RID), 19923726 (EID), S1744309109040287 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777034.
Subject(s): Amino Acid Sequence
Animals
Crystallography, X-Ray
Female
Fibroblast Growth Factor 1/chemistry
Humans
Models, Molecular
Molecular Sequence Data
Protein Structure, Secondary
Protein Structure, Tertiary
Rabbits
Sequence Alignment
Sequence Homology, Amino Acid
Thermodynamics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_19923726
Owner Institution: FSU
Is Part Of: Acta crystallographica. Section F, Structural biology and crystallization communications.
1744-3091
Issue: iss. Pt 11, vol. 65

Choose the citation style.
Lee, J., Blaber, S. I., Irsigler, A., Aspinwall, E., & Blaber, M. (2009). X-ray structure and biophysical properties of rabbit fibroblast growth factor 1. Acta Crystallographica. Section F, Structural Biology And Crystallization Communications. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_19923726