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Experimental support for the evolution of symmetric protein architecture from a simple peptide motif.

Title: Experimental support for the evolution of symmetric protein architecture from a simple peptide motif.
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Name(s): Lee, Jihun, author
Blaber, Michael, author
Type of Resource: text
Genre: journal article
text
Date Issued: 2011-01-04
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The majority of protein architectures exhibit elements of structural symmetry, and "gene duplication and fusion" is the evolutionary mechanism generally hypothesized to be responsible for their emergence from simple peptide motifs. Despite the central importance of the gene duplication and fusion hypothesis, experimental support for a plausible evolutionary pathway for a specific protein architecture has yet to be effectively demonstrated. To address this question, a unique "top-down symmetric deconstruction" strategy was utilized to successfully identify a simple peptide motif capable of recapitulating, via gene duplication and fusion processes, a symmetric protein architecture (the threefold symmetric β-trefoil fold). The folding properties of intermediary forms in this deconstruction agree precisely with a previously proposed "conserved architecture" model for symmetric protein evolution. Furthermore, a route through foldable sequence-space between the simple peptide motif and extant protein fold is demonstrated. These results provide compelling experimental support for a plausible evolutionary pathway of symmetric protein architecture via gene duplication and fusion processes.
Identifier: FSU_pmch_21173271 (IID), 10.1073/pnas.1015032108 (DOI), PMC3017207 (PMCID), 21173271 (RID), 21173271 (EID), 1015032108 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017207.
Subject(s): Amino Acid Motifs/genetics
Calorimetry, Differential Scanning
Chromatography, Gel
Crystallography, X-Ray
Evolution, Molecular
Gene Duplication/genetics
Models, Genetic
Models, Molecular
Molecular Sequence Data
Mutagenesis
Peptides/genetics
Protein Conformation
Protein Folding
Ultracentrifugation
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_21173271
Owner Institution: FSU
Is Part Of: Proceedings of the National Academy of Sciences of the United States of America.
1091-6490
Issue: iss. 1, vol. 108

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Lee, J., & Blaber, M. (2011). Experimental support for the evolution of symmetric protein architecture from a simple peptide motif. Proceedings Of The National Academy Of Sciences Of The United States Of America. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_21173271